P0DXN6 · NCAP_MEASF

Function

function

Forms the helical nucleocapsid (NC) in a ratio of 1 N per 6 ribonucleotides, protecting the genome from nucleases (By similarity).
The nucleocapsid (NC) has a helical structure with either 12.35 or 11.64 N per turn, approximately 20 nm in diameter, with a hollow central cavity approximately 5 nm in diameter (By similarity).
The encapsidated genomic RNA serves as template for transcription and replication; encapsidation by N is coupled to RNA synthesis (By similarity).
Forms the encapsidation complex with the phosphoprotein protein P (PubMed:26719278).
Before encapsidation, the newly synthesized free N protein, so-called N0, is chaperoned by P (By similarity).
Participates, together with P, in the formation of viral factories (viroplasms), which are large inclusions in the host cytoplasm where replication takes place (By similarity).
N is released in the blood following lysis of measles infected cells, it interacts then with human FCGR2B on immune cells, inducing apoptosis and blocking inflammatory immune response (PubMed:15914856).

Caution

The strains used in this publication turn out to be Edmonston strain viruses, contrasting with their claimed origin from reconsituted measles virus genomic cDNAs containing a genetic tag.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site180RNA (UniProtKB | ChEBI)
Binding site195RNA (UniProtKB | ChEBI)
Binding site202RNA (UniProtKB | ChEBI)
Binding site260RNA (UniProtKB | ChEBI)
Binding site351RNA (UniProtKB | ChEBI)

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Nucleoprotein
  • Alternative names
    • Nucleocapsid protein (NP; Protein N)

Gene names

    • Name
      N
    • Synonyms
      NP

Organism names

Accessions

  • Primary accession
    P0DXN6
  • Secondary accessions
    • O92927
    • Q77M21
    • Q77M26
    • Q77M32
    • Q783Q8

Proteomes

Subcellular Location

Virion
Host cytoplasm
Host nucleus

Keywords

Phenotypes & Variants

Features

Showing features for natural variant.

TypeIDPosition(s)Description
Natural variant58
Natural variant129
Natural variant137
Natural variant148
Natural variant262
Natural variant405
Natural variant434
Natural variant448
Natural variant465
Natural variant474
Natural variant478
Natural variant479
Natural variant483
Natural variant487
Natural variant493-494
Natural variant516
Natural variant521
Natural variant522

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 18 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain, modified residue.

Type
IDPosition(s)Description
ChainPRO_00001426551-525Nucleoprotein
Modified residue279Phosphothreonine; by host

Post-translational modification

Phosphorylation at Thr-279 is required for the formation of the nucleocapsid.

Keywords

Interaction

Subunit

Homomultimer; forms the nucleocapsid (By similarity).
Binds to viral genomic RNA (By similarity).
N0 interacts (via Ncore) with the phosphoprotein (via N-terminus); this interaction allows P to chaperon N0 to avoid N polymerization and non-specific RNA binding before encapsidation (PubMed:26719278).
Interacts (via the Ntail) as N-RNA template with the phosphoprotein (via C-terminus XD); this interaction maintains the P/L complex anchored to the nucleocapsid template during the sequential transcription (By similarity).
Interacts with the phosphoprotein; this interaction leads to the formation of membraneless organelles that function as viral replication factories (By similarity).
Interacts (via Ncore) with human FCGR2B protein (PubMed:15914856).
Interacts (via Ntail) with a protein on human thymic epithelial cells, termed Nucleoprotein Receptor (NR); this interaction induces growth arrest (PubMed:15914856, PubMed:14557619).
Interacts with human PPIA/CYPA and PPIB/CYPB (By similarity).

Family & Domains

Features

Showing features for region, motif.

Type
IDPosition(s)Description
Region1-36Homomultimerization
Region1-375RNA packaging and organization of the helical nucleocapsid
Region1-403Ncore
Motif70-77Nuclear localization signal
Region373-391Homomultimerization
Region404-525Ntail
Motif425-440Nuclear export signal
Region477-505Interaction with the phosphoprotein

Domain

Ncore is globular and carries regions required for N self-assembly and RNA-binding. Ntail is an intrinsically disordered monomeric domain in the C-terminus.

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    525
  • Mass (Da)
    58,131
  • Last updated
    2024-11-27 v1
  • Checksum
    3C35DF4458769BA0
MATLLRSLALFKRNKDKPPITSGSGGAIRGIKHIIIVPIPGDSSITTRSRLLDRLVRLIGNPDVSGPKLTGALIGILSLFVESPGQLIQRITDDPDVSIRLLEVVQSDQSQSGLTFASRGTNMEDEADQYFSHDDPISSDQSRFGWFENKEISDIEVQDPEGFNMILGTILAQIWVLLAKAVTAPDTAADSELRRWIKYTQQRRVVGEFRLERKWLDVVRNRIAEDLSLRRFMVALILDIKRTPGNKPRIAEMICDIDTYIVEAGLASFILTIKFGIETMYPALGLHEFAGELSTLESLMNLYQQMGETAPYMVILENSIQNKFSAGSYPLLWSYAMGVGVELENSMGGLNFGRSYFDPAYFRLGQEMVRRSAGKVSSTLASELGITAEDARLVSEIAMHTTEDKISRAVGPRQAQVSFLHGDQSENELPRLGGKEDRRVKQSRGEARESYRETGPSRASDARAAHLPTGTPLDIDTASESSQDPQDSRRSADALLRLQAMAGISEEQGSDTDTPIVYNDRNLLD

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
Z66517
EMBL· GenBank· DDBJ
CAA91363.1
EMBL· GenBank· DDBJ
Genomic RNA
U03650
EMBL· GenBank· DDBJ
AAA56640.1
EMBL· GenBank· DDBJ
Genomic RNA
U03653
EMBL· GenBank· DDBJ
AAA56643.1
EMBL· GenBank· DDBJ
Genomic RNA
U03656
EMBL· GenBank· DDBJ
AAA56646.1
EMBL· GenBank· DDBJ
Genomic RNA
U03658
EMBL· GenBank· DDBJ
AAA56648.1
EMBL· GenBank· DDBJ
Genomic RNA
U03661
EMBL· GenBank· DDBJ
AAA56651.1
EMBL· GenBank· DDBJ
Genomic RNA
U01987
EMBL· GenBank· DDBJ
AAA18986.1
EMBL· GenBank· DDBJ
mRNA
U01991
EMBL· GenBank· DDBJ
AAA18990.1
EMBL· GenBank· DDBJ
mRNA
U01992
EMBL· GenBank· DDBJ
AAA18991.1
EMBL· GenBank· DDBJ
mRNA
U01993
EMBL· GenBank· DDBJ
AAA18992.1
EMBL· GenBank· DDBJ
mRNA
U01994
EMBL· GenBank· DDBJ
AAA18993.1
EMBL· GenBank· DDBJ
mRNA
U01996
EMBL· GenBank· DDBJ
AAA18995.1
EMBL· GenBank· DDBJ
mRNA
AB052821
EMBL· GenBank· DDBJ
BAB60956.1
EMBL· GenBank· DDBJ
Genomic RNA
AF045218
EMBL· GenBank· DDBJ
AAC03050.1
EMBL· GenBank· DDBJ
Genomic RNA

Similar Proteins

Disclaimer

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