P0DXF3 · FAPA_PSEFL

  • Protein
    Fibril amyloid chaperone protein FapA
  • Gene
    fapA
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    3/5

Function

function

An intrinsically disordered chaperone for fibril amyloid FapC that guards against fibrillation within the periplasm (PubMed:36368411).
FapA inhibits FapC fibrillation and elongation of FapB fibrils in vitro (PubMed:36368411).
Upon overexpression of the endogenous six-gene locus (fapA-fapF) in situ, cells form large clumps during liquid growth, make large amounts of biofilm and produce amyloid fibrils (PubMed:23504942, PubMed:26500638).
Expression of the 6 gene operon in E.coli strain BL21(DE3) induces flocculation and biofilm formation with copious extracellular fibrils (PubMed:20572935).

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Fibril amyloid chaperone protein FapA

Gene names

    • Name
      fapA
    • ORF names
      HZ99_04080
      , PSUK4_00010

Organism names

  • Taxonomic identifier
  • Strain
    • UK4
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Pseudomonadales > Pseudomonadaceae > Pseudomonas

Accessions

  • Primary accession
    P0DXF3

Subcellular Location

Periplasm

Keywords

Phenotypes & Variants

Disruption phenotype

Deletion of just fapA in an overexpressing strain yields less dense fibrils around the cells (PubMed:26500638).
Deletion of the entire fapA-fapF six-gene locus shows no visible growth phenotype (PubMed:23504942, PubMed:26500638).

PTM/Processing

Features

Showing features for signal, chain.

Type
IDPosition(s)Description
Signal1-28
ChainPRO_000046151329-152Fibril amyloid chaperone protein FapA

Interaction

Subunit

Monomer in solution. Interacts with FapC but not FapB in vitro (PubMed:36368411).

Family & Domains

Sequence similarities

Belongs to the FapA family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    152
  • Mass (Da)
    14,891
  • Last updated
    2024-11-27 v1
  • Checksum
    75CDF55D9EB7CD23
MATLNQGSLSLLLISCVLGASVSVPASAEDGIIVTGRNVQGFMVGRPSFGKDPYPSTANANPSQQILRATGGELSDNDFAGVSSGSTITRAILPNGDLPGLSNTLGNNSAGLGAGAAAGHASGNSLGGQISGSIERGLAPLNNIGAMMGVQP

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
ACOQ01000001
EMBL· GenBank· DDBJ
EEP64549.1
EMBL· GenBank· DDBJ
Genomic DNA
CP008896
EMBL· GenBank· DDBJ
AIG01389.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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