P0DXC2 · RL40_RABIT

  • Protein
    Ubiquitin-ribosomal protein eL40 fusion protein
  • Gene
    UBA52
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Ubiquitin

Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in proteotoxic stress response and cell cycle; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.

Large ribosomal subunit protein eL40

Component of the 60S subunit of the ribosome. Ribosomal protein L40 is essential for translation of a subset of cellular transcripts, and especially for cap-dependent translation of vesicular stomatitis virus mRNAs.

Miscellaneous

Ubiquitin is encoded by 4 different genes. UBA52 and RPS27A genes code for a single copy of ubiquitin fused to the ribosomal proteins eL40 and eS31, respectively. UBB and UBC genes code for a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains.
For a better understanding, features related to ubiquitin are only indicated for the first chain.

Features

Showing features for site.

TypeIDPosition(s)Description
Site54Interacts with activating enzyme
Site68Essential for function
Site72Interacts with activating enzyme

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentnucleus
Cellular Componentribonucleoprotein complex
Cellular Componentribosome
Molecular Functionprotein tag activity
Molecular FunctionRNA binding
Molecular Functionstructural constituent of ribosome
Molecular Functionubiquitin protein ligase binding
Biological Processmodification-dependent protein catabolic process
Biological Processprotein ubiquitination
Biological Processtranslation

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Ubiquitin-ribosomal protein eL40 fusion protein
  • Alternative names
    • Ubiquitin A-52 residue ribosomal protein fusion product 1
  • Cleaved into 2 chains

Gene names

    • Name
      UBA52

Organism names

  • Taxonomic identifier
  • Strain
    • Thorbecke
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Lagomorpha > Leporidae > Oryctolagus

Accessions

  • Primary accession
    P0DXC2

Proteomes

Subcellular Location

Ubiquitin

Cytoplasm
Nucleus

Large ribosomal subunit protein eL40

Cytoplasm

Keywords

PTM/Processing

Features

Showing features for chain, cross-link, modified residue.

TypeIDPosition(s)Description
ChainPRO_00004601331-76Ubiquitin
ChainPRO_00004601321-128Ubiquitin-ribosomal protein eL40 fusion protein
Cross-link6Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
Cross-link11Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
Cross-link27Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
Cross-link29Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
Cross-link33Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
Cross-link48Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
Cross-link63Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
Modified residue65Phosphoserine
Modified residue76ADP-ribosylglycine
Cross-link76Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)
ChainPRO_000046013477-128Large ribosomal subunit protein eL40
Modified residue98N6,N6,N6-trimethyllysine

Post-translational modification

Ubiquitin

Phosphorylated at Ser-65 by PINK1 during mitophagy. Phosphorylated ubiquitin specifically binds and activates parkin (PRKN), triggering mitophagy. Phosphorylation does not affect E1-mediated E2 charging of ubiquitin but affects discharging of E2 enzymes to form polyubiquitin chains. It also affects deubiquitination by deubiquitinase enzymes such as USP30.

Ubiquitin

Mono-ADP-ribosylated at the C-terminus by PARP9, a component of the PPAR9-DTX3L complex. ADP-ribosylation requires processing by E1 and E2 enzymes and prevents ubiquitin conjugation to substrates such as histones.

Large ribosomal subunit protein eL40

Trimethylation of Lys-98 ('Lys-22' of the mature chain) by SMYD5 promotes translation elongation and protein synthesis.

Keywords

Interaction

Subunit

Ribosomal protein L40 is part of the 60S ribosomal subunit (PubMed:35709277, PubMed:36653451).
Interacts with UBQLN1 (via UBA domain) (By similarity).

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain1-76Ubiquitin-like

Sequence similarities

In the N-terminal section; belongs to the ubiquitin family.
In the C-terminal section; belongs to the eukaryotic ribosomal protein eL40 family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    128
  • Mass (Da)
    14,728
  • Last updated
    2024-03-27 v1
  • Checksum
    7BCB602ABEFAD02A
MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGIIEPSLRQLAQKYNCDKMICRKCYARLHPRAVNCRKKKCGHTNNLRPKKKVK

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
VIYN02000755
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.

Similar Proteins

Disclaimer

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