P0DW54 · OSPC1_BORBG
- ProteinOuter surface protein C
- GeneospC
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids212 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Major immunodominant protein in mammalian hosts (PubMed:7665660).
Required for initial stages of mammalian infection (PubMed:14722614).
Exploits its interaction with Ixodes scapularis salivary protein 15 (Salp15) or its paralog I.ricinus Iric-1 to increase mammalian host infection. Binding of bacteria to Salp15 or Iric-1 protects them from antibody-mediated destruction in vitro, due to interaction with this protein (PubMed:16049492, PubMed:18752445).
Preincubation with Salp15 or Iric-1 allows bacteria to infect mice that had already cleared a B.burgdorferi infection, showing it is important in bacterial colonization and dissemination (PubMed:16049492, PubMed:18752445).
Decreasing Salp15 levels in ticks by RNAi leads to decreased bacterial presence in infected mice (PubMed:16049492).
Inhibits macrophage-mediated phagocytosis of the bacteria. Binds human (host) plasminogen; this probably confers an extracellular protease activity on the bacteria that allows it to traverse host tissue (By similarity).
Required for initial stages of mammalian infection (PubMed:14722614).
Exploits its interaction with Ixodes scapularis salivary protein 15 (Salp15) or its paralog I.ricinus Iric-1 to increase mammalian host infection. Binding of bacteria to Salp15 or Iric-1 protects them from antibody-mediated destruction in vitro, due to interaction with this protein (PubMed:16049492, PubMed:18752445).
Preincubation with Salp15 or Iric-1 allows bacteria to infect mice that had already cleared a B.burgdorferi infection, showing it is important in bacterial colonization and dissemination (PubMed:16049492, PubMed:18752445).
Decreasing Salp15 levels in ticks by RNAi leads to decreased bacterial presence in infected mice (PubMed:16049492).
Inhibits macrophage-mediated phagocytosis of the bacteria. Binds human (host) plasminogen; this probably confers an extracellular protease activity on the bacteria that allows it to traverse host tissue (By similarity).
Miscellaneous
The causative agent of Lyme disease, the bacteria has an enzootic lifestyle. Larval ticks are infected with bacteria during feeding on infected hosts (mostly mammals) which retain the bacteria during subsequent developmental stages. It is transmitted to the next host when it is bitten by ticks. During tick feeding (which can last for several days), bacterial migrate from the tick midgut to the salivary gland where they are transmitted to the host.
This gene is encoded on a 26 kb circular plasmid (cp26) in strains B31, N40 and B.afzelii strain PKo; it is probably encoded on cp26 in the other strains given here.
Strain N40 was originally isolated in 1988 from an I.scapularis tick in eastern New York state by D. Fish; the original tick may have been infected by multiple B.burgdorferi strains, giving rise to multiple, different 'N40' clones.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cell outer membrane | |
Cellular Component | cell surface |
Keywords
- Biological process
Names & Taxonomy
Protein names
- Recommended nameOuter surface protein C
Gene names
Encoded on
- Plasmid cp26
Organism names
- Strains
- Taxonomic lineageBacteria > Spirochaetota > Spirochaetia > Spirochaetales > Borreliaceae > Borreliella
Accessions
- Primary accessionP0DW54
- Secondary accessions
Subcellular Location
Phenotypes & Variants
Disruption phenotype
PTM/Processing
Features
Showing features for signal, lipidation, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-18 | |||||
Sequence: MKKNTLSAILMTLFLFIS | ||||||
Lipidation | 19 | N-palmitoyl cysteine | ||||
Sequence: C | ||||||
Lipidation | 19 | S-diacylglycerol cysteine | ||||
Sequence: C | ||||||
Chain | PRO_0000018084 | 19-212 | Outer surface protein C | |||
Sequence: CNNSGKGGDSASTNPADESAKGPNLTEISKKITDSNAFVLAVKEVETLVLSIDELAKKAIGQKIDNNNGLAALNNQNGSLLAGAYAISTLITEKLSKLKNLEELKTEIAKAKKCSEEFTNKLKSGHADLGKQDATDDHAKAAILKTHATTDKGAKEFKDLFESVEGLLKAAQVALTNSVKELTSPVVAESPKKP |
Keywords
- PTM
Expression
Induction
Poorly expressed in vitro in strain N40, expressed for a short time following mouse infection, then it disappears (at protein level).
Interaction
Subunit
Homodimer (PubMed:11139584, PubMed:11230121).
Binds to tick salivary glands; antibodies against this protein cause a decrease in Borrelia movement to the tick salivary glands during host feeding (PubMed:14722614).
Interacts with host Ixodes scapularis salivary protein 15 (Salp15) (PubMed:16049492, PubMed:18752445).
Interacts with host I.ricinus salivary protein Iric-1 (PubMed:18752445).
Binds human (host) plasminogen (By similarity).
Binds to tick salivary glands; antibodies against this protein cause a decrease in Borrelia movement to the tick salivary glands during host feeding (PubMed:14722614).
Interacts with host Ixodes scapularis salivary protein 15 (Salp15) (PubMed:16049492, PubMed:18752445).
Interacts with host I.ricinus salivary protein Iric-1 (PubMed:18752445).
Binds human (host) plasminogen (By similarity).
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length212
- Mass (Da)22,500
- Last updated2022-10-12 v1
- ChecksumC206C231FBF2E7D4
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 56 | in Ref. 1; X69590 | ||||
Sequence: F → T |
Keywords
- Technical term