P0DW54 · OSPC1_BORBG

Function

function

Major immunodominant protein in mammalian hosts (PubMed:7665660).
Required for initial stages of mammalian infection (PubMed:14722614).
Exploits its interaction with Ixodes scapularis salivary protein 15 (Salp15) or its paralog I.ricinus Iric-1 to increase mammalian host infection. Binding of bacteria to Salp15 or Iric-1 protects them from antibody-mediated destruction in vitro, due to interaction with this protein (PubMed:16049492, PubMed:18752445).
Preincubation with Salp15 or Iric-1 allows bacteria to infect mice that had already cleared a B.burgdorferi infection, showing it is important in bacterial colonization and dissemination (PubMed:16049492, PubMed:18752445).
Decreasing Salp15 levels in ticks by RNAi leads to decreased bacterial presence in infected mice (PubMed:16049492).
Inhibits macrophage-mediated phagocytosis of the bacteria. Binds human (host) plasminogen; this probably confers an extracellular protease activity on the bacteria that allows it to traverse host tissue (By similarity).

Miscellaneous

The causative agent of Lyme disease, the bacteria has an enzootic lifestyle. Larval ticks are infected with bacteria during feeding on infected hosts (mostly mammals) which retain the bacteria during subsequent developmental stages. It is transmitted to the next host when it is bitten by ticks. During tick feeding (which can last for several days), bacterial migrate from the tick midgut to the salivary gland where they are transmitted to the host.
This gene is encoded on a 26 kb circular plasmid (cp26) in strains B31, N40 and B.afzelii strain PKo; it is probably encoded on cp26 in the other strains given here.
Strain N40 was originally isolated in 1988 from an I.scapularis tick in eastern New York state by D. Fish; the original tick may have been infected by multiple B.burgdorferi strains, giving rise to multiple, different 'N40' clones.

GO annotations

AspectTerm
Cellular Componentcell outer membrane
Cellular Componentcell surface

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Outer surface protein C

Gene names

    • Name
      ospC

Encoded on

  • Plasmid cp26

Organism names

Accessions

  • Primary accession
    P0DW54
  • Secondary accessions
    • Q08137
    • Q9S3P4

Subcellular Location

Cell outer membrane
; Lipid-anchor
Cell surface

Keywords

Phenotypes & Variants

Disruption phenotype

No change in ability to colonize the tick gut, greatly decreased migration to the tick salivary gland during feeding, about 800-fold reduction in transmission to mice (PubMed:14722614).
No longer binds host I.scapularis Salp15 protein (strain ATCC 53899 / 297) (PubMed:16049492, PubMed:18752445).

PTM/Processing

Features

Showing features for signal, lipidation, chain.

TypeIDPosition(s)Description
Signal1-18
Lipidation19N-palmitoyl cysteine
Lipidation19S-diacylglycerol cysteine
ChainPRO_000001808419-212Outer surface protein C

Keywords

Expression

Induction

Poorly expressed in vitro in strain N40, expressed for a short time following mouse infection, then it disappears (at protein level).

Interaction

Subunit

Homodimer (PubMed:11139584, PubMed:11230121).
Binds to tick salivary glands; antibodies against this protein cause a decrease in Borrelia movement to the tick salivary glands during host feeding (PubMed:14722614).
Interacts with host Ixodes scapularis salivary protein 15 (Salp15) (PubMed:16049492, PubMed:18752445).
Interacts with host I.ricinus salivary protein Iric-1 (PubMed:18752445).
Binds human (host) plasminogen (By similarity).

Structure

Family & Domains

Sequence similarities

Belongs to the OspC lipoprotein family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    212
  • Mass (Da)
    22,500
  • Last updated
    2022-10-12 v1
  • Checksum
    C206C231FBF2E7D4
MKKNTLSAILMTLFLFISCNNSGKGGDSASTNPADESAKGPNLTEISKKITDSNAFVLAVKEVETLVLSIDELAKKAIGQKIDNNNGLAALNNQNGSLLAGAYAISTLITEKLSKLKNLEELKTEIAKAKKCSEEFTNKLKSGHADLGKQDATDDHAKAAILKTHATTDKGAKEFKDLFESVEGLLKAAQVALTNSVKELTSPVVAESPKKP

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict56in Ref. 1; X69590

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X69590
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
X73624
EMBL· GenBank· DDBJ
CAA52003.1
EMBL· GenBank· DDBJ
Genomic DNA
L42883
EMBL· GenBank· DDBJ
AAB36991.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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