P0DUF6 · BRXA_ECOHS

Function

function

BREX systems (bacteriophage exclusion) provide immunity against bacteriophage. A probably non-essential part of a type 1 BREX system which protects against dsDNA phage. This system allows phage adsorption but prevents phage DNA replication, without degradation of the phage DNA. Methylation of bacterial DNA by PglX guides self/non-self discrimination. When the brxA-brxB-brxC-pglX-pglZ-brxL genes are transformed into a susceptible E.coli strain (BW25113) they confer very high resistance to infection by bacteriophage VR7 and VpaE1, about 100-fold protection against lambda, T5 and T7 and no protection against RNA phage Qbeta, ssDNA phage M13 or dSDNA phage T4 and VR5. Glycosylated phage DNA is not susceptible to BREX. The BREX system does not confer resistance to lysogenic lambda phage, i.e. prophage that are integrated into the chromosomal DNA and then induced to form phage.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Biological Processdefense response to virus

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    BREX protein BrxA

Gene names

    • Name
      brxA
    • Ordered locus names
      EcHS_A0336

Organism names

Accessions

  • Primary accession
    P0DUF6
  • Secondary accessions
    • A0A7M3S2P1

Proteomes

Phenotypes & Variants

Disruption phenotype

No visible phenotype, host DNA is methylated on fifth position of 5'-GGTAAG-3' in chromosomal DNA, BREX still confers phage resistance.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004521531-213BREX protein BrxA

Expression

Induction

Transcribed at slowly increasing levels as cells progress from lag to exponential to stationary phase.

Structure

Family & Domains

Sequence similarities

Belongs to the BrxA family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    213
  • Mass (Da)
    23,999
  • Last updated
    2021-06-02 v1
  • Checksum
    E0206DF190E696FA
MRAAFQSSEGFSMIKNDKAWIGDLLGGPLMSRESRVIAELLLTDPDEQTWQEQIVGHNILQASSPNTAKRYAATIRLRLNTLDKSAWTLIAEGSERERQQLLFVALMLHSPVVKDFLAEVVNDLRRQFKEKLPGNSWNEFVNSQVRLHPVLASYSDSSIAKMGNNLVKALAEAGYVDTPRRRNLQAVYLLPETQAVLQRLGQQDLISILEGKR

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP000802
EMBL· GenBank· DDBJ
ABV04724.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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