P0DTS9 · TIRS_STAAS

Function

function

Virulence factor that suppresses host Toll-like receptor 2 (TLR2)-mediated NF-kappa-B signaling upon infection (PubMed:24481289, PubMed:28060920).
NAD+ hydrolase (NADase) that catalyzes cleavage of NAD+ into ADP-D-ribose (ADPR) and nicotinamide (PubMed:29395922).
Also able to hydrolyze NADP+, but not other NAD+-related molecules (PubMed:29395922).
Able to reduce NAD+ levels in host cells (PubMed:29395922).

Catalytic activity

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
490 μMNAD+

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site150-151NAD+ (UniProtKB | ChEBI)
Binding site180NAD+ (UniProtKB | ChEBI)
Active site216

GO annotations

AspectTerm
Cellular Componentextracellular region
Molecular FunctionNAD+ nucleosidase activity
Molecular FunctionNAD+ nucleotidase, cyclic ADP-ribose generating
Molecular FunctionNADP+ nucleosidase activity
Biological ProcessNAD catabolic process
Biological Processsignal transduction

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    NAD(+) hydrolase TirS
  • EC number
  • Alternative names
    • NADP(+) hydrolase TirS
      (EC:3.2.2.-
      ) . EC:3.2.2.- (UniProtKB | ENZYME | Rhea)
    • TIR domain-containing protein in S.aureus

Gene names

    • Name
      tirS
    • Ordered locus names
      SAS0038

Organism names

Accessions

  • Primary accession
    P0DTS9

Subcellular Location

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis216Loss of NAD+ hydrolase activity.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004491461-280NAD+ hydrolase TirS

Structure

Family & Domains

Features

Showing features for coiled coil, domain.

TypeIDPosition(s)Description
Coiled coil22-94
Domain141-275TIR

Domain

The TIR domain mediates NAD+ hydrolase (NADase) activity. Self-association of TIR domains is required for NADase activity.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    280
  • Mass (Da)
    32,811
  • Last updated
    2020-02-26 v1
  • Checksum
    AF8B2752C7CC67E1
MSVLETKLKSQMSKSAKIARNMNKLPDEIDRLRKRIERINKKRKPTSSNIRDLEKSNKQLVTKQQKLADLQVEYTKIEKKINETKINLQKEQSRNQKKLSSMLDKNTKGNEEIMEKLLTNSDQINEISNQIKKAVNQKEIIEYDVFLSHSSLDKEDYVSKISEKLIEKGLKVFEDVKVFEIGKSQTETMNMGILNSRFVVVFLSPNFIESGWSRYEFLSFLNREINEEHVIILPIWHKVSVEDVRAYNPYLVDKYALNTSDFSIEEIVEKIYQVIVNSKN

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
BX571857
EMBL· GenBank· DDBJ
CAG41810.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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