P0DQQ1 · 3L23_NAJME
- ProteinLong neurotoxin Tx-NM2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Binds with high affinity to muscular (alpha-1-beta-1-gamma-delta/CHRNA1-CHRNB1-CHRNG-CHRND) and neuronal (alpha-7/CHRNA7) nicotinic acetylcholine receptor (nAChR) and inhibits acetylcholine from binding to the receptor, thereby impairing neuromuscular and neuronal transmission (PubMed:33672715).
Ranges of nAChR inhibition are in nanomolar (competitive binding with alpha-bungarotoxin gives Ki=8.66 nM on muscle nAChR and Ki=9.47 nM on alpha-7) (PubMed:33672715).
Also shows moderate inhibition on GABA(A) alpha-1-beta-3-gamma-2 receptor (GABRA1-GABRB3-GABRG2) (IC50=1.25 uM), and a lower inhibition on alpha-1-beta-2-gamma-2 (GABRA1-GABRB2-GABRG2) and alpha-3-beta-2-gamma-2 (GABRA3-GABRB2-GABRG2) (PubMed:33672715).
Ranges of nAChR inhibition are in nanomolar (competitive binding with alpha-bungarotoxin gives Ki=8.66 nM on muscle nAChR and Ki=9.47 nM on alpha-7) (PubMed:33672715).
Also shows moderate inhibition on GABA(A) alpha-1-beta-3-gamma-2 receptor (GABRA1-GABRB3-GABRG2) (IC50=1.25 uM), and a lower inhibition on alpha-1-beta-2-gamma-2 (GABRA1-GABRB2-GABRG2) and alpha-3-beta-2-gamma-2 (GABRA3-GABRB2-GABRG2) (PubMed:33672715).
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | extracellular region | |
Molecular Function | acetylcholine receptor inhibitor activity | |
Molecular Function | ion channel regulator activity | |
Molecular Function | toxin activity |
Keywords
- Molecular function
Names & Taxonomy
Protein names
- Recommended nameLong neurotoxin Tx-NM2
Organism names
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Lepidosauria > Squamata > Bifurcata > Unidentata > Episquamata > Toxicofera > Serpentes > Colubroidea > Elapidae > Elapinae > Naja
Accessions
- Primary accessionP0DQQ1
Subcellular Location
PTM/Processing
Features
Showing features for chain, disulfide bond.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000453025 | 1-72 | Long neurotoxin Tx-NM2 | ||
Disulfide bond | 3↔21 | ||||
Disulfide bond | 14↔42 | ||||
Disulfide bond | 27↔31 | ||||
Disulfide bond | 46↔57 | ||||
Disulfide bond | 58↔63 | ||||
Keywords
- PTM
Expression
Tissue specificity
Expressed by the venom gland.
Structure
Family & Domains
Sequence similarities
Belongs to the snake three-finger toxin family. Long-chain subfamily. Type II alpha-neurotoxin sub-subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length72
- Mass (Da)8,127
- Last updated2021-06-02 v1
- Checksum47F6AD9542434208
Mass Spectrometry
Keywords
- Technical term