P0DO61 · NO40A_MEDTR

Function

function

Modulates the action of auxin, and may function as plant growth regulator that alters phytohormone responses (Probable). During symbiosis with rhizobacteria such as Sinorhizobium meliloti SM2011, in response to Nod factors and under nitrogen limitation, involved in nodule initiation by inducing dedifferentiation and division of root cortical cells (primordium formation), and essential for bacteroid development during nodulation; this processus is repressed by ethylene (PubMed:10521525, PubMed:11038563, PubMed:11113209, PubMed:17452749).
During symbiosis with arbuscular mycorrhizal fungi such as Glomus mosseae and G.intraradices, promotes mycorrhizal colonization in the root cortex and enhances the formation of arbuscules (PubMed:11752473).
Involved in root-knot nematode Meloidogyne incognita giant cells formation (PubMed:12437298).

Miscellaneous

Enod40 mRNA triggers RBP1 translocation to cytoplasmic granules from the nucleus during nodule development.

GO annotations

all annotationsall molecular functionnucleotide bindingmolecular_functionnucleic acid bindingdna bindingchromatin bindingdna-binding transcription factor activityrna bindingcytoskeletal motor activitycatalytic activitynuclease activitysignaling receptor bindingstructural molecule activitytransporter activitybindingprotein bindingtranslation factor activity, rna bindinglipid bindingkinase activitytransferase activityhydrolase activityoxygen bindingenzyme regulator activitycarbohydrate bindingsignaling receptor activitytranslation regulator activitytranscription regulator activityother molecular functionall biological processcarbohydrate metabolic processgeneration of precursor metabolites and energynucleobase-containing compound metabolic processdna metabolic processtranslationlipid metabolic processtransportresponse to stresscell cyclecell communicationsignal transductioncell-cell signalingmulticellular organism developmentcircadian rhythmbiological_processmetabolic processcatabolic processbiosynthetic processresponse to light stimulusresponse to external stimulustropismresponse to biotic stimulusresponse to abiotic stimulusresponse to endogenous stimulusembryo developmentpost-embryonic developmentfruit ripeningabscissionpollinationflower developmentcellular processprogrammed cell deathphotosynthesiscellular component organizationcell growthprotein metabolic processcellular homeostasissecondary metabolic processreproductive processcell differentiationprotein modification processgrowthepigenetic regulation of gene expressionresponse to chemicalanatomical structure developmentregulation of molecular functionother biological processall cellular componentcellular_componentextracellular regioncell wallintracellular anatomical structurenucleusnuclear envelopenucleoplasmnucleoluscytoplasmmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuscytosolribosomecytoskeletonplasma membranechloroplastplastidthylakoidmembraneexternal encapsulating structureother cellular component
Cell color indicative of number of GO terms
AspectTerm
Biological Processarbuscular mycorrhizal association
Biological Processnodulation
Biological Processorgan induction
Biological Processresponse to nematode
Biological Processresponse to symbiotic bacterium
Biological Processresponse to symbiotic fungus

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Early nodulin-40-1
  • Short names
    Mtenod4O

Gene names

    • Name
      ENOD40-1

Organism names

  • Taxonomic identifier
  • Strains
    • cv. Ghor
    • cv. Jemalong A17
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > fabids > Fabales > Fabaceae > Papilionoideae > 50 kb inversion clade > NPAAA clade > Hologalegina > IRL clade > Trifolieae > Medicago

Accessions

  • Primary accession
    P0DO61

Proteomes

Phenotypes & Variants

Disruption phenotype

Reduced nodule number after inoculation with Sinorhizobium meliloti SM2011; remaining nodules are abnormally spherical and contain a few small rod-shaped bacteroids, their development is impaired (PubMed:10521525, PubMed:17452749).
Bacteroids development is arrested at stage II-III and they undergo premature senescence in cells of the fixation zone leading to the formation of vacuole-like structures as a result of symbiosomes fusion (PubMed:17452749).
Reduced mycorrhizal colonization by arbuscular mycorrhizal fungi (e.g. Glomus mosseae and G.intraradices) (PubMed:11752473).

PTM/Processing

Features

Showing features for peptide.

TypeIDPosition(s)Description
PeptidePRO_00004578591-13Early nodulin-40-1

Expression

Tissue specificity

Expressed at low levels in roots, stems and flowers (PubMed:7957074).
Accumulates in nodules at a high level (PubMed:15037734).

Induction

Accumulates in response to Sinorhizobium meliloti inoculation leading to nodulation (PubMed:15516512, PubMed:7957074).
Induced by the root-knot nematode Meloidogyne incognita at the nematode feeding sites (NFS), and accumulates in subsequent root galls (PubMed:12437298).

Developmental stage

During root development, weakly expressed in the vascular cylinder (PubMed:12437298).
Expressed in all differentiating cells of nodule primordia (in Nod factor-treated roots) and spontaneous nodules but fades out in differentiated nodule cells (PubMed:7957074).
Also observed around nodule vascular tissues (PubMed:7957074).
After infection with the root-knot nematode Meloidogyne incognita, expressed in cell layers surrounding giant cells (PubMed:12437298).

Family & Domains

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete

This entry describes 2 isoforms produced by Alternative initiation. Both ORFs I and II are required to induce cortical cell division upon Sinorhizobium meliloti inoculation.

P0DO61-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Name
    1
  • Synonyms
    ORF-I
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Length
    13
  • Mass (Da)
    1,532
  • Last updated
    2023-05-03 v1
  • Checksum
    3C6953C4BD8A3B03
MKLLCWEKSIHGS

P0DO61-2

  • Name
    2
  • Synonyms
    ORF-II
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical
    • 2-13: KLLCWEKSIHGS → ANRQVTKRQWIPFWSLNGYVSITLSM

Features

Showing features for alternative sequence.

TypeIDPosition(s)Description
Alternative sequenceVSP_0618482-13in isoform 2

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X80264
EMBL· GenBank· DDBJ
-mRNA No translation available.
CM001221
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.

Similar Proteins

Disclaimer

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