P0DO61 · NO40A_MEDTR
- ProteinEarly nodulin-40-1
- GeneENOD40-1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
Modulates the action of auxin, and may function as plant growth regulator that alters phytohormone responses (Probable). During symbiosis with rhizobacteria such as Sinorhizobium meliloti SM2011, in response to Nod factors and under nitrogen limitation, involved in nodule initiation by inducing dedifferentiation and division of root cortical cells (primordium formation), and essential for bacteroid development during nodulation; this processus is repressed by ethylene (PubMed:10521525, PubMed:11038563, PubMed:11113209, PubMed:17452749).
During symbiosis with arbuscular mycorrhizal fungi such as Glomus mosseae and G.intraradices, promotes mycorrhizal colonization in the root cortex and enhances the formation of arbuscules (PubMed:11752473).
Involved in root-knot nematode Meloidogyne incognita giant cells formation (PubMed:12437298).
During symbiosis with arbuscular mycorrhizal fungi such as Glomus mosseae and G.intraradices, promotes mycorrhizal colonization in the root cortex and enhances the formation of arbuscules (PubMed:11752473).
Involved in root-knot nematode Meloidogyne incognita giant cells formation (PubMed:12437298).
Miscellaneous
Enod40 mRNA triggers RBP1 translocation to cytoplasmic granules from the nucleus during nodule development.
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Biological Process | arbuscular mycorrhizal association | |
Biological Process | nodulation | |
Biological Process | organ induction | |
Biological Process | response to nematode | |
Biological Process | response to symbiotic bacterium | |
Biological Process | response to symbiotic fungus |
Keywords
- Biological process
Names & Taxonomy
Protein names
- Recommended nameEarly nodulin-40-1
- Short namesMtenod4O
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > fabids > Fabales > Fabaceae > Papilionoideae > 50 kb inversion clade > NPAAA clade > Hologalegina > IRL clade > Trifolieae > Medicago
Accessions
- Primary accessionP0DO61
Proteomes
Phenotypes & Variants
Disruption phenotype
Reduced nodule number after inoculation with Sinorhizobium meliloti SM2011; remaining nodules are abnormally spherical and contain a few small rod-shaped bacteroids, their development is impaired (PubMed:10521525, PubMed:17452749).
Bacteroids development is arrested at stage II-III and they undergo premature senescence in cells of the fixation zone leading to the formation of vacuole-like structures as a result of symbiosomes fusion (PubMed:17452749).
Reduced mycorrhizal colonization by arbuscular mycorrhizal fungi (e.g. Glomus mosseae and G.intraradices) (PubMed:11752473).
Bacteroids development is arrested at stage II-III and they undergo premature senescence in cells of the fixation zone leading to the formation of vacuole-like structures as a result of symbiosomes fusion (PubMed:17452749).
Reduced mycorrhizal colonization by arbuscular mycorrhizal fungi (e.g. Glomus mosseae and G.intraradices) (PubMed:11752473).
PTM/Processing
Features
Showing features for peptide.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Peptide | PRO_0000457859 | 1-13 | Early nodulin-40-1 | |||
Sequence: MKLLCWEKSIHGS |
Expression
Tissue specificity
Expressed at low levels in roots, stems and flowers (PubMed:7957074).
Accumulates in nodules at a high level (PubMed:15037734).
Accumulates in nodules at a high level (PubMed:15037734).
Induction
Accumulates in response to Sinorhizobium meliloti inoculation leading to nodulation (PubMed:15516512, PubMed:7957074).
Induced by the root-knot nematode Meloidogyne incognita at the nematode feeding sites (NFS), and accumulates in subsequent root galls (PubMed:12437298).
Induced by the root-knot nematode Meloidogyne incognita at the nematode feeding sites (NFS), and accumulates in subsequent root galls (PubMed:12437298).
Developmental stage
During root development, weakly expressed in the vascular cylinder (PubMed:12437298).
Expressed in all differentiating cells of nodule primordia (in Nod factor-treated roots) and spontaneous nodules but fades out in differentiated nodule cells (PubMed:7957074).
Also observed around nodule vascular tissues (PubMed:7957074).
After infection with the root-knot nematode Meloidogyne incognita, expressed in cell layers surrounding giant cells (PubMed:12437298).
Expressed in all differentiating cells of nodule primordia (in Nod factor-treated roots) and spontaneous nodules but fades out in differentiated nodule cells (PubMed:7957074).
Also observed around nodule vascular tissues (PubMed:7957074).
After infection with the root-knot nematode Meloidogyne incognita, expressed in cell layers surrounding giant cells (PubMed:12437298).
Structure
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative initiation. Both ORFs I and II are required to induce cortical cell division upon Sinorhizobium meliloti inoculation.
P0DO61-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- SynonymsORF-I
- Length13
- Mass (Da)1,532
- Last updated2023-05-03 v1
- Checksum3C6953C4BD8A3B03
P0DO61-2
- Name2
- SynonymsORF-II
- Differences from canonical
- 2-13: KLLCWEKSIHGS → ANRQVTKRQWIPFWSLNGYVSITLSM
Features
Showing features for alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_061848 | 2-13 | in isoform 2 | |||
Sequence: KLLCWEKSIHGS → ANRQVTKRQWIPFWSLNGYVSITLSM |
Keywords
- Coding sequence diversity
- Technical term