P0DMC7 · RCSB_ECOLI

Function

function

Component of the Rcs signaling system, which controls transcription of numerous genes. RcsB is the response regulator that binds to regulatory DNA regions. Can function both in an RcsA-dependent or RcsA-independent manner. The system regulates expression of numerous genes, including genes involved in colanic acid capsule synthesis, biofilm formation, cell division and outer membrane proteins synthesis. Also involved, with GadE, in control of glutamate-dependent acid resistance, and, with BglJ, in derepression of the cryptic bgl operon. The RcsB-BglJ activity is probably independent of RcsB phosphorylation.

Miscellaneous

There is a close linkage between the Rcs and PhoQ/P signaling systems, and both signaling systems respond to certain external divalent cations (zinc and magnesium).

Activity regulation

Activity is modulated by phosphorylation and interaction with other transcriptional regulators. Probably up-regulated by YmgA/AriR, and possibly down-regulated by YcgZ, all 3 are connector proteins providing additional signal input into the signaling system.

Features

Showing features for dna binding.

121620406080100120140160180200
TypeIDPosition(s)Description
DNA binding168-187H-T-H motif

GO annotations

AspectTerm
Cellular Componentcytosol
Cellular Componenttranscription regulator complex
Molecular FunctionDNA binding
Molecular FunctionDNA-binding transcription activator activity
Molecular FunctionDNA-binding transcription repressor activity
Molecular Functionidentical protein binding
Biological Processcellular stress response to acidic pH
Biological ProcessDNA-templated transcription
Biological Processnegative regulation of DNA-templated transcription
Biological Processphosphorelay signal transduction system
Biological Processpositive regulation of cell projection organization
Biological Processpositive regulation of DNA-templated transcription
Biological Processregulation of bacterial-type flagellum-dependent cell motility
Biological Processregulation of capsule organization
Biological Processregulation of carbohydrate catabolic process
Biological Processregulation of DNA-templated transcription
Biological Processresponse to antibiotic
Biological Processsingle-species biofilm formation on inanimate substrate

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Transcriptional regulatory protein RcsB
  • Alternative names
    • Capsular synthesis regulator component B

Gene names

    • Name
      rcsB
    • Ordered locus names
      b2217, JW2205

Organism names

  • Taxonomic identifier
  • Strains
    • K12
    • K12 / W3110 / ATCC 27325 / DSM 5911
    • K12 / MG1655 / ATCC 47076
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia

Accessions

  • Primary accession
    P0DMC7
  • Secondary accessions
    • P14374
    • P69407

Proteomes

Subcellular Location

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis56Increases heterodimer formation with RcsA. Does not affect heterodimer formation with BglJ.
Mutagenesis56Decreases heterodimer formation with RcsA. Does not affect heterodimer formation with BglJ.

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00000812091-216Transcriptional regulatory protein RcsB
Modified residue564-aspartylphosphate

Post-translational modification

Phosphorylated and activated by RcsD.

Keywords

Proteomic databases

Expression

Induction

Expression is dependent on the alternate sigma factor, RpoN (PubMed:2404948).
Repressed by hydroxyurea

Interaction

Subunit

Interacts with RcsD and RcsA. Homodimer. Can form an heterodimer with the coactivator RcsA, which binds to the RcsAB boxes to promote transcription of RcsA-dependent genes. Can also form a heterodimer with GadE to bind to the GAD box, and with BglJ to bind to the bgl promoter.

Binary interactions

Complex viewer

View interactors in UniProtKB
View CPX-5762 in Complex Portal

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain5-124Response regulatory
Domain144-209HTH luxR-type

Sequence similarities

Belongs to the RcsB family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    216
  • Mass (Da)
    23,671
  • Last updated
    2014-02-19 v1
  • Checksum
    A78D1BD3004E0680
MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINNLPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLDIEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLRLFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
M28242
EMBL· GenBank· DDBJ
AAA24504.1
EMBL· GenBank· DDBJ
Genomic DNA
U00096
EMBL· GenBank· DDBJ
AAC75277.1
EMBL· GenBank· DDBJ
Genomic DNA
AP009048
EMBL· GenBank· DDBJ
BAA16000.1
EMBL· GenBank· DDBJ
Genomic DNA
S37760
EMBL· GenBank· DDBJ
AAB22290.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp