P0DMC7 · RCSB_ECOLI
- ProteinTranscriptional regulatory protein RcsB
- GenercsB
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids216 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Component of the Rcs signaling system, which controls transcription of numerous genes. RcsB is the response regulator that binds to regulatory DNA regions. Can function both in an RcsA-dependent or RcsA-independent manner. The system regulates expression of numerous genes, including genes involved in colanic acid capsule synthesis, biofilm formation, cell division and outer membrane proteins synthesis. Also involved, with GadE, in control of glutamate-dependent acid resistance, and, with BglJ, in derepression of the cryptic bgl operon. The RcsB-BglJ activity is probably independent of RcsB phosphorylation.
Miscellaneous
There is a close linkage between the Rcs and PhoQ/P signaling systems, and both signaling systems respond to certain external divalent cations (zinc and magnesium).
Activity regulation
Activity is modulated by phosphorylation and interaction with other transcriptional regulators. Probably up-regulated by YmgA/AriR, and possibly down-regulated by YcgZ, all 3 are connector proteins providing additional signal input into the signaling system.
Features
Showing features for dna binding.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
DNA binding | 168-187 | H-T-H motif | ||||
Sequence: VTEIAKKLNRSIKTISSQKK |
GO annotations
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameTranscriptional regulatory protein RcsB
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionP0DMC7
- Secondary accessions
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 56 | Increases heterodimer formation with RcsA. Does not affect heterodimer formation with BglJ. | ||||
Sequence: D → E | ||||||
Mutagenesis | 56 | Decreases heterodimer formation with RcsA. Does not affect heterodimer formation with BglJ. | ||||
Sequence: D → N |
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000081209 | 1-216 | Transcriptional regulatory protein RcsB | |||
Sequence: MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINNLPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLDIEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLRLFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD | ||||||
Modified residue | 56 | 4-aspartylphosphate | ||||
Sequence: D |
Post-translational modification
Phosphorylated and activated by RcsD.
Keywords
- PTM
Proteomic databases
Expression
Induction
Expression is dependent on the alternate sigma factor, RpoN (PubMed:2404948).
Repressed by hydroxyurea
Repressed by hydroxyurea
Interaction
Subunit
Interacts with RcsD and RcsA. Homodimer. Can form an heterodimer with the coactivator RcsA, which binds to the RcsAB boxes to promote transcription of RcsA-dependent genes. Can also form a heterodimer with GadE to bind to the GAD box, and with BglJ to bind to the bgl promoter.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P0DMC7 | bglJ P39404 | 4 | EBI-369670, EBI-551429 | |
BINARY | P0DMC7 | dctR P37195 | 3 | EBI-369670, EBI-562540 | |
BINARY | P0DMC7 | rcsB P0DMC7 | 3 | EBI-369670, EBI-369670 | |
BINARY | P0DMC7 | rcsD P39838 | 5 | EBI-369670, EBI-556803 |
Complex viewer
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 5-124 | Response regulatory | ||||
Sequence: NVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINNLPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLDIEGIVLKQGAPTDLPKALAALQ | ||||||
Domain | 144-209 | HTH luxR-type | ||||
Sequence: GGYGDKRLSPKESEVLRLFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVT |
Sequence similarities
Belongs to the RcsB family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length216
- Mass (Da)23,671
- Last updated2014-02-19 v1
- ChecksumA78D1BD3004E0680
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
M28242 EMBL· GenBank· DDBJ | AAA24504.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U00096 EMBL· GenBank· DDBJ | AAC75277.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP009048 EMBL· GenBank· DDBJ | BAA16000.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
S37760 EMBL· GenBank· DDBJ | AAB22290.1 EMBL· GenBank· DDBJ | Genomic DNA |