P0DM59 · UNAG_ANGJA
- ProteinBilirubin-inducible fluorescent protein UnaG
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids139 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Beta-barrel protein that binds unconjugated bilirubin with high affinity. Excitation of the bilirubin-bound protein gives rise to green fluorescence, both under normoxia and hypoxia. The apoprotein is not fluorescent. Does not emit fluorescence in the presence of ditauro-bilirubin, urobilin or biliverdin.
Biotechnology
Becomes fluorescent upon binding free, unconjugated bilirubin, a heme metabolite. Can be used as diagnostic tool to detect abnormally high levels of free bilirubin, which are indicative of impaired liver function. Could also be used in research as fluorescent marker in eukaryotic cells and animals.
Absorption
Excitation of the bilirubin-bound protein gives rise to green fluorescence, with maximal fluorecence emission at 527 nm.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 57 | (4Z,15Z)-bilirubin IXalpha (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 61 | (4Z,15Z)-bilirubin IXalpha (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 80 | (4Z,15Z)-bilirubin IXalpha (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 112 | (4Z,15Z)-bilirubin IXalpha (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 132-134 | (4Z,15Z)-bilirubin IXalpha (UniProtKB | ChEBI) | ||||
Sequence: RSY |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | nucleus | |
Molecular Function | fatty acid binding | |
Biological Process | bioluminescence | |
Biological Process | fatty acid transport |
Keywords
- Molecular function
- Biological process
Names & Taxonomy
Protein names
- Recommended nameBilirubin-inducible fluorescent protein UnaG
- Alternative names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Actinopterygii > Neopterygii > Teleostei > Anguilliformes > Anguillidae > Anguilla
Accessions
- Primary accessionP0DM59
Subcellular Location
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 57 | Abolishes fluorescence emission. | ||||
Sequence: N → A | ||||||
Mutagenesis | 57 | Reduces fluorescence emission. | ||||
Sequence: N → Q |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000423436 | 1-139 | Bilirubin-inducible fluorescent protein UnaG | |||
Sequence: MVEKFVGTWKIADSHNFGEYLKAIGAPKELSDGGDATTPTLYISQKDGDKMTVKIENGPPTFLDTQVKFKLGEEFDEFPSDRRKGVKSVVNLVGEKLVYVQKWDGKETTYVREIKDGKLVVTLTMGDVVAVRSYRRATE |
Expression
Tissue specificity
Detected in small-diameter muscle fibers from the white muscle layer from juvenile animals (glass eels) (at protein level). Detected in small-diameter muscle fibers from juvenile animals (glass eels).
Interaction
Subunit
Monomer.
Structure
Family & Domains
Domain
Forms a beta-barrel structure that accommodates bilirubin in its interior.
Sequence similarities
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length139
- Mass (Da)15,581
- Last updated2013-09-18 v1
- Checksum1F6C6410DFE25009
Keywords
- Technical term