P0DKH3 · RI2BL_ARATH
- ProteinRibonucleoside-diphosphate reductase small chain B
- GeneRNR2B
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids333 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.
Catalytic activity
- a 2'-deoxyribonucleoside 5'-diphosphate + [thioredoxin]-disulfide + H2O = a ribonucleoside 5'-diphosphate + [thioredoxin]-dithiol
CHEBI:73316 + RHEA-COMP:10700 CHEBI:50058 Position: n/n+3+ CHEBI:15377 = CHEBI:57930 + RHEA-COMP:10698 CHEBI:29950 Position: nCHEBI:29950 Position: n+3
Cofactor
Note: Binds 2 iron ions per subunit.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 76 | Fe cation 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 107 | Fe cation 1 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 107 | Fe cation 2 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 110 | Fe cation 1 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Active site | 114 | |||||
Sequence: Y | ||||||
Binding site | 169 | Fe cation 2 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 203 | Fe cation 2 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 206 | Fe cation 2 (UniProtKB | ChEBI) | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | metal ion binding | |
Molecular Function | ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor | |
Biological Process | deoxyribonucleotide biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameRibonucleoside-diphosphate reductase small chain B
- EC number
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionP0DKH3
- Secondary accessions
Subcellular Location
Phenotypes & Variants
Disruption phenotype
No visible phenotype, due to the redundancy with RNR2A and TSO2. Rnr2a and rnr2b double mutants have no visible phenotype.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000430464 | 1-333 | Ribonucleoside-diphosphate reductase small chain B | |||
Sequence: MPSMPEEPILTPTPDRFCMFPIQYPQIWEMYKKAEASFWTAEEVDLSQDNRDWENSLTNDERHFIKHVLAFFAASDGIVLENLSTRFMSDVQISEARAFYGFQIAIENIHSEMYSLLLDTYIKDNKERDHLFRAIETIPCVTKKAEWAMKWINGSQSFAERIVAFACVEGIFFSGSFCSIFWLKKRGLMPGLTFSNELISRDEGLHCDFACLIYSLLRTKLDEDRLKAIVCDAVEIEREFVCDALPCALVGMNRELMSQYIEFVADRLLAALGCAKVYGVSNPFDWMELISLQGKTNFFEKRVGEYQKASIMSSVHGNAAFNDDHVFKLDEDF |
Expression
Tissue specificity
Expressed in roots, rosette leaves, stems and flowers.
Gene expression databases
Interaction
Subunit
Heterodimer of a large and a small chain.
Structure
Sequence
- Sequence statusComplete
- Length333
- Mass (Da)38,331
- Last updated2014-10-01 v1
- ChecksumCA20B044F92DB922