P0DJG7 · VSP2_DEIAC

Function

function

Thrombin-like snake venom serine protease that has high fibrinogen-clotting activity (2025 NIH units/mg) on human fibrinogen. Shows esterase and amidase activities.

Activity regulation

Inhibited by PMSF (100%), AEBSF (100%), aprotinin (50%), benzamidine (50%), guanidinium chloride (48%) and urea (66%).

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
403 μMBAEE7.025
68.5 nMDL-BAPA7.537
Vmax pH TEMPERATURE[C] NOTES EVIDENCE
1560 nmol/sec/mg7.025
490 nmol/min/mg7.537with DL-BAPA as substrate

pH Dependence

Optimum pH is 9.0 (at 25 degrees Celsius).

Temperature Dependence

Optimum temperature is 25-50 degrees Celsius (at pH 7.0).

GO annotations

AspectTerm
Cellular Componentextracellular region
Molecular Functionserine-type peptidase activity
Molecular Functiontoxin activity
Biological Processproteolysis

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Thrombin-like enzyme acutobin-2
  • EC number
  • Short names
    SVTLE
  • Alternative names
    • Acutobin II
    • Fibrinogen-clotting enzyme
    • Snake venom serine protease (SVSP)

Organism names

Accessions

  • Primary accession
    P0DJG7

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain, disulfide bond.

TypeIDPosition(s)Description
ChainPRO_00004170081-15Thrombin-like enzyme acutobin-2
Disulfide bond7↔?

Post-translational modification

Seems to be only O-glycosylated (is not affected by PNGase F which only removes N-linked glycans, but is affected by TFMS that removes both O- and N-linked glycans).

Keywords

Expression

Tissue specificity

Expressed by the venom gland.

Interaction

Subunit

Monomer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain1-15Peptidase S1

Sequence similarities

Belongs to the peptidase S1 family. Snake venom subfamily.

Family and domain databases

Sequence

  • Sequence status
    Fragment
  • Length
    15
  • Mass (Da)
    1,701
  • Last updated
    2012-05-16 v1
  • Checksum
    81F387D827D52FD4
VIGGVECDINEHRFL

Features

Showing features for non-terminal residue.

TypeIDPosition(s)Description
Non-terminal residue15

Mass Spectrometry

Molecular mass is 34,069.5 Da. Determined by MALDI.

Keywords

Similar Proteins

Disclaimer

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