P0DJG7 · VSP2_DEIAC
- ProteinThrombin-like enzyme acutobin-2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Thrombin-like snake venom serine protease that has high fibrinogen-clotting activity (2025 NIH units/mg) on human fibrinogen. Shows esterase and amidase activities.
Activity regulation
Inhibited by PMSF (100%), AEBSF (100%), aprotinin (50%), benzamidine (50%), guanidinium chloride (48%) and urea (66%).
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
403 μM | BAEE | 7.0 | 25 | |||
68.5 nM | DL-BAPA | 7.5 | 37 |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
1560 nmol/sec/mg | 7.0 | 25 | |||
490 nmol/min/mg | 7.5 | 37 | with DL-BAPA as substrate |
pH Dependence
Optimum pH is 9.0 (at 25 degrees Celsius).
Temperature Dependence
Optimum temperature is 25-50 degrees Celsius (at pH 7.0).
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | extracellular region | |
Molecular Function | serine-type peptidase activity | |
Molecular Function | toxin activity | |
Biological Process | proteolysis |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameThrombin-like enzyme acutobin-2
- EC number
- Short namesSVTLE
- Alternative names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Lepidosauria > Squamata > Bifurcata > Unidentata > Episquamata > Toxicofera > Serpentes > Colubroidea > Viperidae > Crotalinae > Deinagkistrodon
Accessions
- Primary accessionP0DJG7
Subcellular Location
PTM/Processing
Features
Showing features for chain, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000417008 | 1-15 | Thrombin-like enzyme acutobin-2 | |||
Sequence: VIGGVECDINEHRFL | ||||||
Disulfide bond | 7↔? | |||||
Sequence: VIGGVE |
Post-translational modification
Seems to be only O-glycosylated (is not affected by PNGase F which only removes N-linked glycans, but is affected by TFMS that removes both O- and N-linked glycans).
Keywords
- PTM
Expression
Tissue specificity
Expressed by the venom gland.
Interaction
Subunit
Monomer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 1-15 | Peptidase S1 | ||||
Sequence: VIGGVECDINEHRFL |
Sequence similarities
Belongs to the peptidase S1 family. Snake venom subfamily.
Family and domain databases
Sequence
- Sequence statusFragment
- Length15
- Mass (Da)1,701
- Last updated2012-05-16 v1
- Checksum81F387D827D52FD4
Features
Showing features for non-terminal residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Non-terminal residue | 15 | |||||
Sequence: L |
Mass Spectrometry
Keywords
- Technical term