P0DJ44 · VM3_VIPAA
- ProteinZinc metalloproteinase-disintegrin-like VaH1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Snake venom zinc metalloprotease that exhibits strong hemorrhagic activity. It also degrades alpha-chain of fibrinogen (FGA), but not the beta- and the gamma-chains. Possesses potent azocaseinolytic activity and cleaves insulin B-chain, hydrolyzing it at positions Ala14-Leu15, followed by Tyr16-Leu17 and His10-Leu11. In vivo, subcutaneous injection into mice induces strong hemorrhage.
Cofactor
Note: Binds 1 zinc ion per subunit.
Activity regulation
Inhibited by EDTA, but not inhibited by iodoacetamide, PMSF and pepstatin A.
pH Dependence
Optimum pH is 7.5.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | extracellular region | |
Cellular Component | host extracellular space | |
Molecular Function | metal ion binding | |
Molecular Function | metallopeptidase activity | |
Molecular Function | toxin activity | |
Biological Process | envenomation resulting in fibrinogenolysis in another organism | |
Biological Process | envenomation resulting in hemorrhagic damage in another organism | |
Biological Process | proteolysis |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameZinc metalloproteinase-disintegrin-like VaH1
- EC number
- Alternative names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Lepidosauria > Squamata > Bifurcata > Unidentata > Episquamata > Toxicofera > Serpentes > Colubroidea > Viperidae > Viperinae > Vipera
Accessions
- Primary accessionP0DJ44
Subcellular Location
PTM/Processing
Features
Showing features for chain, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000418197 | 1-36 | Zinc metalloproteinase-disintegrin-like VaH1 | |||
Sequence: MVTKYSSIFMSPILSNPPILYFSDCSREXYQKXLTN | ||||||
Disulfide bond | 25↔? | |||||
Sequence: MVTKYSSIFMSPILSNPPILYFSD |
Post-translational modification
The N-terminus is blocked.
Glycosylated.
Keywords
- PTM
Expression
Tissue specificity
Expressed by the venom gland.
Interaction
Subunit
Monomer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 1-36 | Peptidase M12B | ||||
Sequence: MVTKYSSIFMSPILSNPPILYFSDCSREXYQKXLTN |
Sequence similarities
Family and domain databases
Sequence
- Sequence statusFragments
- Length36
- Mass (Da)4,197
- Last updated2012-07-11 v1
- ChecksumB96368629ACCD153
Features
Showing features for non-terminal residue, non-adjacent residues.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Non-terminal residue | 1 | |||||
Sequence: M | ||||||
Non-adjacent residues | 9-10 | |||||
Sequence: FM | ||||||
Non-terminal residue | 36 | |||||
Sequence: N |
Keywords
- Technical term