P0CY08 · MTAL2_YEAST

Function

function

Mating type proteins are sequence specific DNA-binding proteins that act as master switches in yeast differentiation by controlling gene expression in a cell type-specific fashion. Transcriptional corepressor that binds cooperatively with MCM1 to a 31-basepair DNA sequence termed the a-specific gene (asg) operator, to repress the transcription of a-cell-specific genes. Additionally, in a/alpha diploid cells, binds cooperatively with the A1 protein to a 21-basepair DNA sequence termed the haploid-specific gene (hsg) operator, to repress transcription of haploid-specific genes and of MATALPHA1.

Miscellaneous

There are three genetic loci for mating type genes in S.cerevisiae. MAT is the expression locus that determines the mating type of the cell, whereas HML (containing HMLALPHA1 and HMLALPHA2) and HMR (containing HMRA1 and HMRA2) represent silenced repositories of mating type information. The mating type is determined by the MAT locus, which contains either a copy of HML or of HMR. Diploid cells are usually heterozygous for the MAT locus.

Features

Showing features for dna binding.

121020406080100120140160180200
TypeIDPosition(s)Description
DNA binding129-191Homeobox; TALE-type

GO annotations

AspectTerm
Cellular Componentnucleus
Cellular ComponentRNA polymerase II transcription repressor complex
Molecular FunctionDNA binding, bending
Molecular FunctionDNA-binding transcription factor activity, RNA polymerase II-specific
Molecular FunctionDNA-binding transcription repressor activity, RNA polymerase II-specific
Molecular FunctionRNA polymerase II cis-regulatory region sequence-specific DNA binding
Molecular Functionsequence-specific DNA binding
Biological Processnegative regulation of transcription by RNA polymerase II
Biological Processregulation of mating-type specific transcription, DNA-templated
Biological Processregulation of transcription by RNA polymerase II

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Mating-type protein ALPHA2
  • Short names
    MATalpha2 protein
  • Alternative names
    • Alpha-2 repressor

Gene names

    • Name
      MATALPHA2
    • Synonyms
      ALPHA-2, MAT2A, MATAL2
    • ORF names
      YCR39C
    • Ordered locus names
      YCR039C

Organism names

Accessions

  • Primary accession
    P0CY08
  • Secondary accessions
    • D6VQV2
    • P01367
    • P01368
    • Q6B2C0

Proteomes

Organism-specific databases

Subcellular Location

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis4Reduces the ability of ALPHA2 to repress transcription, but binds normally to DNA and MCM1.
Mutagenesis9Reduces the ability of ALPHA2 to repress transcription, but binds normally to DNA and MCM1.
Mutagenesis10Reduces the ability of ALPHA2 to repress transcription, but binds normally to DNA and MCM1. Disrupts interaction with TUP1.
Mutagenesis71Reduces the ability of alpha2 to repress transcription, but binds normally to DNA and MCM1.
Mutagenesis114Reduces the ability of ALPHA2/MCM1 to repress a-specific genes.
Mutagenesis115Reduces the ability of ALPHA2/MCM1 to repress a-specific genes.
Mutagenesis116Reduces the ability of ALPHA2/MCM1 to repress a-specific genes.
Mutagenesis117Reduces the ability of ALPHA2/MCM1 to repress a-specific genes.
Mutagenesis118Reduces the ability of ALPHA2/MCM1 to repress a-specific genes.
Mutagenesis119Reduces the ability of ALPHA2/MCM1 to repress a-specific genes.
Mutagenesis120Reduces the ability of ALPHA2/MCM1 to repress a-specific genes.
Mutagenesis173Reduces the ability of ALPHA2/MCM1 to repress a-specific genes. Disrupts interaction with SSN6.
Mutagenesis181In ALPHA2-3A; defective in binding DNA alone or in complex with MCM1, but binds DNA normally in complex with A1; when associated with A-182 and A-185.
Mutagenesis182In ALPHA2-3A; defective in binding DNA alone or in complex with MCM1, but binds DNA normally in complex with A1; when associated with A-181 and A-185.
Mutagenesis185In ALPHA2-3A; defective in binding DNA alone or in complex with MCM1, but binds DNA normally in complex with A1; when associated with A-181 and A-182.
Mutagenesis192Disrupts the ability of A1/ALPHA2 to repress haploid-specific genes.
Mutagenesis196Disrupts the ability of A1/ALPHA2 to repress haploid-specific genes.
Mutagenesis199Disrupts the ability of A1/ALPHA2 to repress haploid-specific genes.
Mutagenesis200Disrupts the ability of A1/ALPHA2 to repress haploid-specific genes.

Variants

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The viewer provides 1 variant from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for modified residue, chain.

TypeIDPosition(s)Description
Modified residue1N-acetylmethionine
ChainPRO_00000491861-210Mating-type protein ALPHA2

Keywords

Proteomic databases

PTM databases

Expression

Developmental stage

Only present in alpha-cells and in a/alpha diploid cells.

Gene expression databases

Interaction

Subunit

Binds DNA with a high specificity as a heterotetramer consisting of an ALPHA2 dimer and an MCM1 dimer. Also binds DNA with a high specificity as a heterodimer of A1 and ALPHA2 in a/alpha diploid cells. Interacts with the general transcription repressor complex SSN6/TUP1.

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY P0CY08MCM1 P117462EBI-10443, EBI-10528
View interactors in UniProtKB
View CPX-684 in Complex Portal

Protein-protein interaction databases

Miscellaneous

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region1-102N-terminal domain
Region103-128Flexible linker
Region190-210C-terminal tail

Domain

The N-terminal domain is required for the interaction with the WD repeats of TUP1 and for dimerization.
The homeobox domain binds to DNA and interacts with the TPR repeats of SSN6.
The unstructured, flexible linker domain contains eight residues (Leu-114 to Gln-121), which, in the presence of MCM1, adopt a beta-fold and mediate the cooperative interaction to MCM1.
The C-terminal tail domain is disordered in the monomer, even when bound to DNA. In the ternary complex with A1 and DNA, 16 residues (Ile-190 to Leu-205) of the C-terminal tail undergo a conformational change, becoming ordered and contacting the A1 homeodomain.

Sequence similarities

Belongs to the TALE/M-ATYP homeobox family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    210
  • Mass (Da)
    24,282
  • Last updated
    2011-06-28 v1
  • Checksum
    30CD6A486D7D76CE
MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDEEKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKENVRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict56in Ref. 1; AAA34762
Sequence conflict150in Ref. 1; AAA34762
Sequence conflict154in Ref. 5; AAT92829

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
L00060
EMBL· GenBank· DDBJ
AAA34762.1
EMBL· GenBank· DDBJ
Genomic DNA
X63853
EMBL· GenBank· DDBJ
CAA45335.1
EMBL· GenBank· DDBJ
Genomic DNA
X59720
EMBL· GenBank· DDBJ
CAA42306.1
EMBL· GenBank· DDBJ
Genomic DNA
AY692810
EMBL· GenBank· DDBJ
AAT92829.1
EMBL· GenBank· DDBJ
Genomic DNA
BK006937
EMBL· GenBank· DDBJ
DAA07518.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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