P0CY08 · MTAL2_YEAST
- ProteinMating-type protein ALPHA2
- GeneMATALPHA2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids210 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Mating type proteins are sequence specific DNA-binding proteins that act as master switches in yeast differentiation by controlling gene expression in a cell type-specific fashion. Transcriptional corepressor that binds cooperatively with MCM1 to a 31-basepair DNA sequence termed the a-specific gene (asg) operator, to repress the transcription of a-cell-specific genes. Additionally, in a/alpha diploid cells, binds cooperatively with the A1 protein to a 21-basepair DNA sequence termed the haploid-specific gene (hsg) operator, to repress transcription of haploid-specific genes and of MATALPHA1.
Miscellaneous
There are three genetic loci for mating type genes in S.cerevisiae. MAT is the expression locus that determines the mating type of the cell, whereas HML (containing HMLALPHA1 and HMLALPHA2) and HMR (containing HMRA1 and HMRA2) represent silenced repositories of mating type information. The mating type is determined by the MAT locus, which contains either a copy of HML or of HMR. Diploid cells are usually heterozygous for the MAT locus.
Features
Showing features for dna binding.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
DNA binding | 129-191 | Homeobox; TALE-type | ||||
Sequence: KPYRGHRFTKENVRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTIT |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | nucleus | |
Cellular Component | RNA polymerase II transcription repressor complex | |
Molecular Function | DNA binding, bending | |
Molecular Function | DNA-binding transcription factor activity, RNA polymerase II-specific | |
Molecular Function | DNA-binding transcription repressor activity, RNA polymerase II-specific | |
Molecular Function | RNA polymerase II cis-regulatory region sequence-specific DNA binding | |
Molecular Function | sequence-specific DNA binding | |
Biological Process | negative regulation of transcription by RNA polymerase II | |
Biological Process | regulation of mating-type specific transcription, DNA-templated | |
Biological Process | regulation of transcription by RNA polymerase II |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameMating-type protein ALPHA2
- Short namesMATalpha2 protein
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP0CY08
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 4 | Reduces the ability of ALPHA2 to repress transcription, but binds normally to DNA and MCM1. | ||||
Sequence: I → T | ||||||
Mutagenesis | 9 | Reduces the ability of ALPHA2 to repress transcription, but binds normally to DNA and MCM1. | ||||
Sequence: L → F | ||||||
Mutagenesis | 10 | Reduces the ability of ALPHA2 to repress transcription, but binds normally to DNA and MCM1. Disrupts interaction with TUP1. | ||||
Sequence: L → S | ||||||
Mutagenesis | 71 | Reduces the ability of alpha2 to repress transcription, but binds normally to DNA and MCM1. | ||||
Sequence: E → K | ||||||
Mutagenesis | 114 | Reduces the ability of ALPHA2/MCM1 to repress a-specific genes. | ||||
Sequence: L → A | ||||||
Mutagenesis | 115 | Reduces the ability of ALPHA2/MCM1 to repress a-specific genes. | ||||
Sequence: V → A | ||||||
Mutagenesis | 116 | Reduces the ability of ALPHA2/MCM1 to repress a-specific genes. | ||||
Sequence: F → A | ||||||
Mutagenesis | 117 | Reduces the ability of ALPHA2/MCM1 to repress a-specific genes. | ||||
Sequence: N → A | ||||||
Mutagenesis | 118 | Reduces the ability of ALPHA2/MCM1 to repress a-specific genes. | ||||
Sequence: V → A | ||||||
Mutagenesis | 119 | Reduces the ability of ALPHA2/MCM1 to repress a-specific genes. | ||||
Sequence: V → A | ||||||
Mutagenesis | 120 | Reduces the ability of ALPHA2/MCM1 to repress a-specific genes. | ||||
Sequence: T → A | ||||||
Mutagenesis | 173 | Reduces the ability of ALPHA2/MCM1 to repress a-specific genes. Disrupts interaction with SSN6. | ||||
Sequence: R → A | ||||||
Mutagenesis | 181 | In ALPHA2-3A; defective in binding DNA alone or in complex with MCM1, but binds DNA normally in complex with A1; when associated with A-182 and A-185. | ||||
Sequence: S → A | ||||||
Mutagenesis | 182 | In ALPHA2-3A; defective in binding DNA alone or in complex with MCM1, but binds DNA normally in complex with A1; when associated with A-181 and A-185. | ||||
Sequence: N → A | ||||||
Mutagenesis | 185 | In ALPHA2-3A; defective in binding DNA alone or in complex with MCM1, but binds DNA normally in complex with A1; when associated with A-181 and A-182. | ||||
Sequence: R → A | ||||||
Mutagenesis | 192 | Disrupts the ability of A1/ALPHA2 to repress haploid-specific genes. | ||||
Sequence: I → A | ||||||
Mutagenesis | 196 | Disrupts the ability of A1/ALPHA2 to repress haploid-specific genes. | ||||
Sequence: L → A or S | ||||||
Mutagenesis | 199 | Disrupts the ability of A1/ALPHA2 to repress haploid-specific genes. | ||||
Sequence: L → A | ||||||
Mutagenesis | 200 | Disrupts the ability of A1/ALPHA2 to repress haploid-specific genes. | ||||
Sequence: L → A |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 1 variant from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for modified residue, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Modified residue | 1 | N-acetylmethionine | ||||
Sequence: M | ||||||
Chain | PRO_0000049186 | 1-210 | Mating-type protein ALPHA2 | |||
Sequence: MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDEEKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKENVRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Developmental stage
Only present in alpha-cells and in a/alpha diploid cells.
Gene expression databases
Interaction
Subunit
Binds DNA with a high specificity as a heterotetramer consisting of an ALPHA2 dimer and an MCM1 dimer. Also binds DNA with a high specificity as a heterodimer of A1 and ALPHA2 in a/alpha diploid cells. Interacts with the general transcription repressor complex SSN6/TUP1.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P0CY08 | MCM1 P11746 | 2 | EBI-10443, EBI-10528 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-102 | N-terminal domain | ||||
Sequence: MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDEEKKLLQTTSQLTTTITVLLKEMRSIENDRSNY | ||||||
Region | 103-128 | Flexible linker | ||||
Sequence: QLTQKNKSADGLVFNVVTQDMINKST | ||||||
Region | 190-210 | C-terminal tail | ||||
Sequence: ITIAPELADLLSGEPLAKKKE |
Domain
The N-terminal domain is required for the interaction with the WD repeats of TUP1 and for dimerization.
The homeobox domain binds to DNA and interacts with the TPR repeats of SSN6.
The unstructured, flexible linker domain contains eight residues (Leu-114 to Gln-121), which, in the presence of MCM1, adopt a beta-fold and mediate the cooperative interaction to MCM1.
The C-terminal tail domain is disordered in the monomer, even when bound to DNA. In the ternary complex with A1 and DNA, 16 residues (Ile-190 to Leu-205) of the C-terminal tail undergo a conformational change, becoming ordered and contacting the A1 homeodomain.
Sequence similarities
Belongs to the TALE/M-ATYP homeobox family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length210
- Mass (Da)24,282
- Last updated2011-06-28 v1
- Checksum30CD6A486D7D76CE
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 56 | in Ref. 1; AAA34762 | ||||
Sequence: G → L | ||||||
Sequence conflict | 150 | in Ref. 1; AAA34762 | ||||
Sequence: K → KK | ||||||
Sequence conflict | 154 | in Ref. 5; AAT92829 | ||||
Sequence: N → S |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
L00060 EMBL· GenBank· DDBJ | AAA34762.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
X63853 EMBL· GenBank· DDBJ | CAA45335.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
X59720 EMBL· GenBank· DDBJ | CAA42306.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY692810 EMBL· GenBank· DDBJ | AAT92829.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006937 EMBL· GenBank· DDBJ | DAA07518.1 EMBL· GenBank· DDBJ | Genomic DNA |