P0CS27 · VPS27_CRYNB
- ProteinVacuolar protein sorting-associated protein 27
- GeneVPS27
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids750 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Component of the ESCRT-0 complex which is the sorting receptor for ubiquitinated cargo proteins at the multivesicular body (MVB) and recruits ESCRT-I to the MVB outer membrane.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 194 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 197 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 224 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 227 | Zn2+ (UniProtKB | ChEBI) | |||
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | early endosome | |
Cellular Component | endosome membrane | |
Molecular Function | metal ion binding | |
Molecular Function | phosphatidylinositol binding | |
Molecular Function | ubiquitin binding | |
Biological Process | endocytic recycling | |
Biological Process | intracellular protein transport | |
Biological Process | receptor internalization | |
Biological Process | vacuolar transport |
Keywords
- Ligand
Names & Taxonomy
Protein names
- Recommended nameVacuolar protein sorting-associated protein 27
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Basidiomycota > Agaricomycotina > Tremellomycetes > Tremellales > Cryptococcaceae > Cryptococcus > Cryptococcus neoformans species complex
Accessions
- Primary accessionP0CS27
- Secondary accessions
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Endosome membrane ; Peripheral membrane protein
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000410326 | 1-750 | Vacuolar protein sorting-associated protein 27 | ||
Interaction
Subunit
Component of the ESCRT-0 complex composed of HSE1 and VPS27.
Structure
Family & Domains
Features
Showing features for domain, zinc finger, region, compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 24-153 | VHS | |||
Zinc finger | 172-232 | FYVE-type; degenerate | |||
Domain | 253-272 | UIM 1 | |||
Region | 268-292 | Disordered | |||
Domain | 294-313 | UIM 2 | |||
Region | 314-336 | Disordered | |||
Region | 447-530 | Disordered | |||
Compositional bias | 473-523 | Polar residues | |||
Region | 564-739 | Disordered | |||
Compositional bias | 600-639 | Polar residues | |||
Compositional bias | 651-712 | Polar residues | |||
Domain
The FYVE domain is involved in the binding to phosphatidylinositol 3-phosphate (PtdIns3P) which is required for the association to endosomal membranes.
Both IUM domains are necessary for efficient binding to ubiquitin.
Sequence similarities
Belongs to the VPS27 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length750
- Mass (Da)82,859
- Last updated2011-06-28 v1
- MD5 Checksum41FE9B2C9D384928091D32FC4FF4DF89
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 473-523 | Polar residues | |||
Compositional bias | 600-639 | Polar residues | |||
Compositional bias | 651-712 | Polar residues | |||
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AAEY01000028 EMBL· GenBank· DDBJ | EAL20449.1 EMBL· GenBank· DDBJ | Genomic DNA |