P0CR30 · SAR1_CRYNJ
- ProteinSmall COPII coat GTPase SAR1
- GeneSAR1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids189 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Small GTPase component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules. SAR1 controls the coat assembly in a stepwise manner. Activated SAR1-GTP binds to membranes first and recruits the SEC23/24 complex. These SEC23/24-SAR1 prebudding intermediates are then collected by the SEC13/31 complex as subunits polymerize to form coated transport vesicles. Conversion to SAR1-GDP triggers coat release and recycles COPII subunits (By similarity).
Catalytic activity
- GTP + H2O = GDP + phosphate + H+
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | COPII vesicle coat | |
Cellular Component | endoplasmic reticulum exit site | |
Cellular Component | endoplasmic reticulum membrane | |
Cellular Component | Golgi membrane | |
Molecular Function | GTP binding | |
Molecular Function | GTPase activity | |
Biological Process | endoplasmic reticulum to Golgi vesicle-mediated transport | |
Biological Process | intracellular protein transport | |
Biological Process | membrane organization | |
Biological Process | positive regulation of protein exit from endoplasmic reticulum | |
Biological Process | regulation of COPII vesicle coating | |
Biological Process | vesicle organization |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSmall COPII coat GTPase SAR1
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Basidiomycota > Agaricomycotina > Tremellomycetes > Tremellales > Cryptococcaceae > Cryptococcus > Cryptococcus neoformans species complex
Accessions
- Primary accessionP0CR30
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Cytoplasmic vesicle, COPII-coated vesicle membrane ; Peripheral membrane protein
Endoplasmic reticulum membrane ; Peripheral membrane protein
Golgi apparatus membrane ; Peripheral membrane protein
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000295513 | 1-189 | Small COPII coat GTPase SAR1 | |||
Sequence: MFIINWFWDVLASLGLMNKSAKLLFLGLDNAGKTTLLHMLKNDRLATLQPTLHPTSEELAIGNVKFTTYDLGGHIQARRLWRDYFPEVDGIVFLVDSADAERFAESKAELDSLLSIESLAQVPFLILGNKIDAYGAVSEEQLRHELGLYQTTGKGKIPLRDIRPIEVFMCSVVMRQGYGEGFRWLSQYI |
Proteomic databases
Interaction
Subunit
COPII is composed of at least 5 proteins: the SEC23/24 complex, the SEC13/31 complex and SAR1.
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length189
- Mass (Da)21,382
- Last updated2011-06-28 v1
- Checksum2FD8A8A2FD050098
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE017342 EMBL· GenBank· DDBJ | AAW41610.1 EMBL· GenBank· DDBJ | Genomic DNA |