P0CK02 · MVP_PPVNA

Function

function

Helper component proteinase

Required for aphid transmission and also has proteolytic activity. Only cleaves a Gly-Gly dipeptide at its own C-terminus. Interacts with virions and aphid stylets. Acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs. May have RNA-binding activity.

Movement protein P3N-PIPO

Allows efficient cell to cell propagation, by bypassing the host cell wall barrier. Transports viral genome to neighboring plant cells directly through plasmosdesmata, without any budding.

Catalytic activity

Features

Showing features for active site, site.

110201002003004005006007008009001,000
TypeIDPosition(s)Description
Active site216For P1 proteinase activity
Active site225For P1 proteinase activity
Active site259For P1 proteinase activity
Site308-309Cleavage; by P1 proteinase
Active site652For helper component proteinase activity
Active site725For helper component proteinase activity
Site766-767Cleavage; by autolysis

GO annotations

AspectTerm
Cellular Componenthost cell plasmodesma
Molecular Functioncysteine-type endopeptidase activity
Molecular Functionserine-type peptidase activity
Biological Processproteolysis
Biological Processsymbiont-mediated suppression of host innate immune response
Biological Processtransport of virus in host, cell to cell
Biological Processviral translational frameshifting
Biological Processvirus-mediated perturbation of host defense response

Keywords

Names & Taxonomy

Protein names

Organism names

Accessions

  • Primary accession
    P0CK02

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004200731-308P1 protease
ChainPRO_00004200721-1020P3N-PIPO polyprotein
ChainPRO_0000420074309-766Helper component proteinase
ChainPRO_0000408546767-1020Movement protein P3N-PIPO

Post-translational modification

Potyviral RNA is expressed as two polyproteins which undergo post-translational proteolytic processing. Genome polyprotein is processed by NIa-pro, P1 and HC-pro proteinases resulting in the production of at least ten individual proteins. P3N-PIPO is cleaved by P1 and HC-pro proteinases resulting in the production of three individual proteins. The P1 proteinase and the HC-pro cleave only their respective C-termini autocatalytically (By similarity).

Interaction

Subunit

Movement protein P3N-PIPO

Interacts (via PIPO domain) with host PCaP1 protein; this interaction may help to anchor the movement complex to the plasma membrane from which the complex could move to the plasmodesmata.

Structure

3D structure databases

Family & Domains

Features

Showing features for domain, motif.

TypeIDPosition(s)Description
Domain165-308Peptidase S30
Motif360-363Involved in interaction with stylet and aphid transmission
Motif618-620Involved in virions binding and aphid transmission
Domain644-766Peptidase C6

Domain

The N-terminus of helper component proteinase is involved in interaction with stylets. The central part is involved in interaction with virions and the C-terminus is involved in cell-to cell movement of the virus (By similarity).

Sequence similarities

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete

This entry describes 2 isoforms produced by Ribosomal frameshifting.

P0CK02-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Name
    P3N-PIPO polyprotein
  • Note
    Produced by -1 ribosomal frameshifting in P3 ORF.
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Length
    1,020
  • Mass (Da)
    116,033
  • Last updated
    2012-10-31 v2
  • Checksum
    966A0FD9DAEEC440
MSTIVFGSFTCHLDAAIHQDNADRLAKAWTRPENRQVSNAHLLCRRAAESLINTYESATASAWKGLEEKLQPMFAKREFSKTVTKRKGLRCFKESSEKFIEKKLRKQYQEERERLQFLNGPDAIVNQISVDKCEASVRVPSPHIIEKPSFVTPSMKKKVVFKKVRMSEASLQLFMRRVAANAKANGQKVEIIGRKRVVGNYTTKSRLTYFRTHVRHLDGSKPRYDLVLDEATKKILQLFANTSGFHHVHKKGEVTPGMSGFVVNPMNLSDPMQVYDTDLFIVRGKHNSILVDSRCKVSKKQSNEIIHYSDPGKQFSDGFTNSFMQCKLRETDHQCTSDLDVKECGYVAALVCQAIIPCGKITCLQCAQKYSYMSQQEIRDRFSTVIEQHEKTVMDNYPQFSHVLAFLKRYRELMRVENQNYEAFKDITHMIGERKEAPFSHLNKINELIIKGGMMSAQDYIEASDHLRELARYQKNRTENIRSGSIKAFRNKISSKAHVNMQLMCDNQLDTNGNFVWGQREYHAKRFFRNYFDVIDVSEGYRRHIVRENPRGIRKLAIGNLVMSTNLAALRKQLLGEECIHFEVSKECTSKRGENFVYQCCCVTHEDGTPLESEIISPTKNHLVVGNSGDSKYVDLPTAKGGAMFIAKAGYCYINIFLAMLININEDEAKSFTKTVRDTLVPKLGTWPSMMDLATACHFLAVLYPETRNAELPRILVDHEAKIFHVVDSFGSLSTGMHVLKANTINQLISFASDTLDSNMKTYLVGGLEVDKCDEFKNVKLLIRSIYKPQIMEQVLKEEPYLLLMSVLSPGVLMALFNSGSLEKATQYWITRSHSLAAITSMLSALAAKVSLASTLNAQMSVIDEHAAVLYDSVFVGTQPYASYMMAVKTLERMKARTESDHTLNDLGFSVLRQATPHLVEKKLSPGIGASLERVKLVGKILCNLGIAAMAKTYTKTFHPKRRRRFRRQVRHLRSVITWQPVQTPERRSPMEKRRCGLLYIPVDGEAILQSHRNLTKFSS

P17766-1

The sequence of this isoform can be found in the external entry linked below. Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

View isoform
  • Name
    Genome polyprotein
  • See also
    sequence in UniParc or sequence clusters in UniRef

Features

Showing features for sequence uncertainty.

TypeIDPosition(s)Description
Sequence uncertainty920-926

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
D13751
EMBL· GenBank· DDBJ
-Genomic RNA No translation available.

Similar Proteins

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