P0CK02 · MVP_PPVNA
- ProteinP3N-PIPO polyprotein
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids1020 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Helper component proteinase
Required for aphid transmission and also has proteolytic activity. Only cleaves a Gly-Gly dipeptide at its own C-terminus. Interacts with virions and aphid stylets. Acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs. May have RNA-binding activity.
Movement protein P3N-PIPO
Allows efficient cell to cell propagation, by bypassing the host cell wall barrier. Transports viral genome to neighboring plant cells directly through plasmosdesmata, without any budding.
Catalytic activity
Helper component proteinase
Hydrolyzes a Gly-|-Gly bond at its own C-terminus, commonly in the sequence -Tyr-Xaa-Val-Gly-|-Gly, in the processing of the potyviral polyprotein.
Features
Showing features for active site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 216 | For P1 proteinase activity | ||||
Sequence: H | ||||||
Active site | 225 | For P1 proteinase activity | ||||
Sequence: D | ||||||
Active site | 259 | For P1 proteinase activity | ||||
Sequence: S | ||||||
Site | 308-309 | Cleavage; by P1 proteinase | ||||
Sequence: YS | ||||||
Active site | 652 | For helper component proteinase activity | ||||
Sequence: C | ||||||
Active site | 725 | For helper component proteinase activity | ||||
Sequence: H | ||||||
Site | 766-767 | Cleavage; by autolysis | ||||
Sequence: GG |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | host cell plasmodesma | |
Molecular Function | cysteine-type endopeptidase activity | |
Molecular Function | serine-type peptidase activity | |
Biological Process | proteolysis | |
Biological Process | symbiont-mediated suppression of host innate immune response | |
Biological Process | transport of virus in host, cell to cell | |
Biological Process | viral translational frameshifting | |
Biological Process | virus-mediated perturbation of host defense response |
Keywords
- Molecular function
- Biological process
Names & Taxonomy
Protein names
- Recommended nameP3N-PIPO polyprotein
- Cleaved into 3 chains
Organism names
- Taxonomic lineageViruses > Riboviria > Orthornavirae > Pisuviricota > Stelpaviricetes > Patatavirales > Potyviridae > Potyvirus > Plum pox virus
- Virus hosts
Accessions
- Primary accessionP0CK02
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Movement protein P3N-PIPO
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000420073 | 1-308 | P1 protease | |||
Sequence: MSTIVFGSFTCHLDAAIHQDNADRLAKAWTRPENRQVSNAHLLCRRAAESLINTYESATASAWKGLEEKLQPMFAKREFSKTVTKRKGLRCFKESSEKFIEKKLRKQYQEERERLQFLNGPDAIVNQISVDKCEASVRVPSPHIIEKPSFVTPSMKKKVVFKKVRMSEASLQLFMRRVAANAKANGQKVEIIGRKRVVGNYTTKSRLTYFRTHVRHLDGSKPRYDLVLDEATKKILQLFANTSGFHHVHKKGEVTPGMSGFVVNPMNLSDPMQVYDTDLFIVRGKHNSILVDSRCKVSKKQSNEIIHY | ||||||
Chain | PRO_0000420072 | 1-1020 | P3N-PIPO polyprotein | |||
Sequence: MSTIVFGSFTCHLDAAIHQDNADRLAKAWTRPENRQVSNAHLLCRRAAESLINTYESATASAWKGLEEKLQPMFAKREFSKTVTKRKGLRCFKESSEKFIEKKLRKQYQEERERLQFLNGPDAIVNQISVDKCEASVRVPSPHIIEKPSFVTPSMKKKVVFKKVRMSEASLQLFMRRVAANAKANGQKVEIIGRKRVVGNYTTKSRLTYFRTHVRHLDGSKPRYDLVLDEATKKILQLFANTSGFHHVHKKGEVTPGMSGFVVNPMNLSDPMQVYDTDLFIVRGKHNSILVDSRCKVSKKQSNEIIHYSDPGKQFSDGFTNSFMQCKLRETDHQCTSDLDVKECGYVAALVCQAIIPCGKITCLQCAQKYSYMSQQEIRDRFSTVIEQHEKTVMDNYPQFSHVLAFLKRYRELMRVENQNYEAFKDITHMIGERKEAPFSHLNKINELIIKGGMMSAQDYIEASDHLRELARYQKNRTENIRSGSIKAFRNKISSKAHVNMQLMCDNQLDTNGNFVWGQREYHAKRFFRNYFDVIDVSEGYRRHIVRENPRGIRKLAIGNLVMSTNLAALRKQLLGEECIHFEVSKECTSKRGENFVYQCCCVTHEDGTPLESEIISPTKNHLVVGNSGDSKYVDLPTAKGGAMFIAKAGYCYINIFLAMLININEDEAKSFTKTVRDTLVPKLGTWPSMMDLATACHFLAVLYPETRNAELPRILVDHEAKIFHVVDSFGSLSTGMHVLKANTINQLISFASDTLDSNMKTYLVGGLEVDKCDEFKNVKLLIRSIYKPQIMEQVLKEEPYLLLMSVLSPGVLMALFNSGSLEKATQYWITRSHSLAAITSMLSALAAKVSLASTLNAQMSVIDEHAAVLYDSVFVGTQPYASYMMAVKTLERMKARTESDHTLNDLGFSVLRQATPHLVEKKLSPGIGASLERVKLVGKILCNLGIAAMAKTYTKTFHPKRRRRFRRQVRHLRSVITWQPVQTPERRSPMEKRRCGLLYIPVDGEAILQSHRNLTKFSS | ||||||
Chain | PRO_0000420074 | 309-766 | Helper component proteinase | |||
Sequence: SDPGKQFSDGFTNSFMQCKLRETDHQCTSDLDVKECGYVAALVCQAIIPCGKITCLQCAQKYSYMSQQEIRDRFSTVIEQHEKTVMDNYPQFSHVLAFLKRYRELMRVENQNYEAFKDITHMIGERKEAPFSHLNKINELIIKGGMMSAQDYIEASDHLRELARYQKNRTENIRSGSIKAFRNKISSKAHVNMQLMCDNQLDTNGNFVWGQREYHAKRFFRNYFDVIDVSEGYRRHIVRENPRGIRKLAIGNLVMSTNLAALRKQLLGEECIHFEVSKECTSKRGENFVYQCCCVTHEDGTPLESEIISPTKNHLVVGNSGDSKYVDLPTAKGGAMFIAKAGYCYINIFLAMLININEDEAKSFTKTVRDTLVPKLGTWPSMMDLATACHFLAVLYPETRNAELPRILVDHEAKIFHVVDSFGSLSTGMHVLKANTINQLISFASDTLDSNMKTYLVG | ||||||
Chain | PRO_0000408546 | 767-1020 | Movement protein P3N-PIPO | |||
Sequence: GLEVDKCDEFKNVKLLIRSIYKPQIMEQVLKEEPYLLLMSVLSPGVLMALFNSGSLEKATQYWITRSHSLAAITSMLSALAAKVSLASTLNAQMSVIDEHAAVLYDSVFVGTQPYASYMMAVKTLERMKARTESDHTLNDLGFSVLRQATPHLVEKKLSPGIGASLERVKLVGKILCNLGIAAMAKTYTKTFHPKRRRRFRRQVRHLRSVITWQPVQTPERRSPMEKRRCGLLYIPVDGEAILQSHRNLTKFSS |
Post-translational modification
Potyviral RNA is expressed as two polyproteins which undergo post-translational proteolytic processing. Genome polyprotein is processed by NIa-pro, P1 and HC-pro proteinases resulting in the production of at least ten individual proteins. P3N-PIPO is cleaved by P1 and HC-pro proteinases resulting in the production of three individual proteins. The P1 proteinase and the HC-pro cleave only their respective C-termini autocatalytically (By similarity).
Interaction
Subunit
Movement protein P3N-PIPO
Interacts (via PIPO domain) with host PCaP1 protein; this interaction may help to anchor the movement complex to the plasma membrane from which the complex could move to the plasmodesmata.
Family & Domains
Features
Showing features for domain, motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 165-308 | Peptidase S30 | ||||
Sequence: RMSEASLQLFMRRVAANAKANGQKVEIIGRKRVVGNYTTKSRLTYFRTHVRHLDGSKPRYDLVLDEATKKILQLFANTSGFHHVHKKGEVTPGMSGFVVNPMNLSDPMQVYDTDLFIVRGKHNSILVDSRCKVSKKQSNEIIHY | ||||||
Motif | 360-363 | Involved in interaction with stylet and aphid transmission | ||||
Sequence: KITC | ||||||
Motif | 618-620 | Involved in virions binding and aphid transmission | ||||
Sequence: PTK | ||||||
Domain | 644-766 | Peptidase C6 | ||||
Sequence: MFIAKAGYCYINIFLAMLININEDEAKSFTKTVRDTLVPKLGTWPSMMDLATACHFLAVLYPETRNAELPRILVDHEAKIFHVVDSFGSLSTGMHVLKANTINQLISFASDTLDSNMKTYLVG |
Domain
The N-terminus of helper component proteinase is involved in interaction with stylets. The central part is involved in interaction with virions and the C-terminus is involved in cell-to cell movement of the virus (By similarity).
Sequence similarities
Belongs to the potyviridae P3N-PIPO polyprotein family.
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Ribosomal frameshifting.
P0CK02-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- NameP3N-PIPO polyprotein
- NoteProduced by -1 ribosomal frameshifting in P3 ORF.
- Length1,020
- Mass (Da)116,033
- Last updated2012-10-31 v2
- Checksum966A0FD9DAEEC440
P17766-1
The sequence of this isoform can be found in the external entry linked below. Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
View isoform- NameGenome polyprotein
Features
Showing features for sequence uncertainty.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence uncertainty | 920-926 | |||||
Sequence: VEKKLSP |
Keywords
- Coding sequence diversity