P0CJ63 · AHLLA_BACTK
- ProteinN-acyl homoserine lactonase AiiA
- GeneaiiA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids250 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes hydrolysis of N-hexanoyl-(S)-homoserine lactone, but not the R-enantiomer. Hydrolyzes short- and long-chain N-acyl homoserine lactones with or without 3-oxo substitution at C3, has maximum activity on C10-AHL.
Catalytic activity
- an N-acyl-L-homoserine lactone + H2O = an N-acyl-L-homoserine + H+
Cofactor
Note: Binds 2 Zn2+ ions per subunit.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
303 mM | GBL (with cobalt as cofactor) | |||||
0.8 mM | C5-HSL (with cobalt as cofactor) | |||||
0.15 mM | C10-HSL (with cobalt as cofactor) | |||||
3.8 mM | BOC-HSL (with cobalt as cofactor) | |||||
0.48 mM | CBZ-HSL (with cobalt as cofactor) |
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 104 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 106 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 108 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 109 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 169 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 191 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 191 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 235 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | acyl-L-homoserine-lactone lactonohydrolase activity | |
Molecular Function | metal ion binding |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameN-acyl homoserine lactonase AiiA
- EC number
- Short namesAHL-lactonase AiiA
Gene names
Organism names
- Strains
- Taxonomic lineageBacteria > Bacillota > Bacilli > Bacillales > Bacillaceae > Bacillus > Bacillus cereus group
Accessions
- Primary accessionP0CJ63
- Secondary accessions
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 104 | Abolishes activity. | ||||
Sequence: H → A | ||||||
Mutagenesis | 106 | Abolishes activity. | ||||
Sequence: H → A | ||||||
Mutagenesis | 108 | Abolishes activity. | ||||
Sequence: D → A | ||||||
Mutagenesis | 108 | Slow substrate turnover. | ||||
Sequence: D → N | ||||||
Mutagenesis | 109 | Reduces activity by 85%. | ||||
Sequence: H → A | ||||||
Mutagenesis | 169 | Abolishes activity. | ||||
Sequence: H → A | ||||||
Mutagenesis | 191 | Abolishes activity. | ||||
Sequence: D → A | ||||||
Mutagenesis | 191 | Abolishes activity. | ||||
Sequence: D → L | ||||||
Mutagenesis | 194 | Reduces activity by 70%. Slow substrate turnover. | ||||
Sequence: Y → F | ||||||
Mutagenesis | 206 | Small decrease in KM value for hydrolysis of GBL, C5-HSL and C10-HSL. | ||||
Sequence: A → W | ||||||
Mutagenesis | 207 | Small decrease in KM value for hydrolysis of GBL and C10-HSL. Increase in KM value for hydrolysis of C5-HSL. | ||||
Sequence: G → D | ||||||
Mutagenesis | 207 | Small decrease in KM value for hydrolysis of GBL and C5-HSL. Larger decrease in KM value for hydrolysis of C10-HSL. | ||||
Sequence: G → W | ||||||
Mutagenesis | 235 | Reduces activity by 85%. | ||||
Sequence: H → A |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000399991 | 1-250 | N-acyl homoserine lactonase AiiA | |||
Sequence: MTVKKLYFIPAGRCMLDHSSVNSALTPGKLLNLPVWCYLLETEEGPILVDTGMPESAVNNEGLFNGTFVEGQILPKMTEEDRIVNILKRVGYEPDDLLYIISSHLHFDHAGGNGAFTNTPIIVQRTEYEAALHREEYMKECILPHLNYKIIEGDYEVVPGVQLLYTPGHSPGHQSLFIETEQSGSVLLTIDASYTKENFEDEVPFAGFDPELALSSIKRLKEVVKKEKPIIFFGHDIEQEKSCRVFPEYI |
Interaction
Subunit
Monomer.
Structure
Sequence
- Sequence statusComplete
- Length250
- Mass (Da)28,220
- Last updated2011-01-11 v1
- ChecksumCEBC2FE957707C2E
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 189 | in Ref. 1; AAL98717 | ||||
Sequence: T → M | ||||||
Sequence conflict | 237 | in Ref. 1; AAL98717 | ||||
Sequence: I → T |
Keywords
- Technical term