P0CI02 · BSD_COCIM
- ProteinBlasticidin-S deaminase
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids137 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Catalyzes the deamination of the cytosine moiety of the antibiotics blasticidin S, cytomycin and acetylblasticidin S.
Catalytic activity
- blasticidin S + H+ + H2O = deaminohydroxyblasticidin S + NH4+
Cofactor
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 31 | substrate; ligand shared between two homotetrameric partners; in other chain | ||||
Sequence: S | ||||||
Binding site | 57 | Zn2+ (UniProtKB | ChEBI); catalytic | ||||
Sequence: C | ||||||
Active site | 59 | Proton donor | ||||
Sequence: E | ||||||
Binding site | 85 | substrate; ligand shared between two homotetrameric partners; in other chain | ||||
Sequence: R | ||||||
Binding site | 91 | Zn2+ (UniProtKB | ChEBI); catalytic | ||||
Sequence: C | ||||||
Binding site | 94 | Zn2+ (UniProtKB | ChEBI); catalytic | ||||
Sequence: C | ||||||
Binding site | 128 | substrate; ligand shared between two homotetrameric partners | ||||
Sequence: Y | ||||||
Binding site | 130 | substrate; ligand shared between two homotetrameric partners; in other chain | ||||
Sequence: W |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | blasticidin-S deaminase activity | |
Molecular Function | cytidine deaminase activity | |
Molecular Function | identical protein binding | |
Molecular Function | zinc ion binding | |
Biological Process | response to antibiotic |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameBlasticidin-S deaminase
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Eurotiomycetes > Eurotiomycetidae > Onygenales > Onygenaceae > Coccidioides
Accessions
- Primary accessionP0CI02
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000399822 | 1-137 | Blasticidin-S deaminase | |||
Sequence: MAPEPLSAAGQNLIDTATSVINGIPVSDFYSVASAAISDDGRVFSGVNVYHFNGGPCAELVVLGVAAAAGATKLTHIVAIANEGRGILSPCGRCRQVLADLHPGIKAIVIGKEGPKMVAVEELLPSIYAWDKDYDRI |
Interaction
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 1-131 | CMP/dCMP-type deaminase | ||||
Sequence: MAPEPLSAAGQNLIDTATSVINGIPVSDFYSVASAAISDDGRVFSGVNVYHFNGGPCAELVVLGVAAAAGATKLTHIVAIANEGRGILSPCGRCRQVLADLHPGIKAIVIGKEGPKMVAVEELLPSIYAWD |
Sequence similarities
Belongs to the cytidine and deoxycytidylate deaminase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length137
- Mass (Da)14,217
- Last updated2010-10-05 v1
- ChecksumF23E4F13D06D8EA0
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
GG704913 EMBL· GenBank· DDBJ | EAS29908.1 EMBL· GenBank· DDBJ | Genomic DNA |