P0C6V8 · R1AB_BRV1

  • Protein
    Replicase polyprotein 1ab
  • Gene
    rep
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at transcript level
  • Annotation score
    5/5

Function

function

3C-like serine proteinase

The 3C-like serine proteinase is responsible for the majority of cleavages.

Helicase

The helicase which contains a zinc finger structure displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity.

Exoribonuclease

Acts on both ssRNA and dsRNA in a 3' to 5' direction.

Uridylate-specific endoribonuclease

NendoU is a Mn2+-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond.

Catalytic activity

Features

Showing features for site, active site, binding site.

TypeIDPosition(s)Description
Site2753-2754Cleavage; by 3C-like serine proteinase
Site3131-3132Cleavage; by 3C-like serine proteinase
Active site3184Charge relay system; for 3C-like serine proteinase activity
Active site3222Charge relay system; for 3C-like serine proteinase activity
Active site3291Charge relay system; for 3C-like serine proteinase activity
Site3418-3419Cleavage; by 3C-like serine proteinase
Site3677-3678Cleavage; by 3C-like serine proteinase
Site3854-3855Cleavage; by 3C-like serine proteinase
Site4036-4037Cleavage; by 3C-like serine proteinase
Site4121-4122Cleavage; by 3C-like serine proteinase
Site4274-4275Cleavage; by 3C-like serine proteinase
Site5286-5287Cleavage; by 3C-like serine proteinase
Binding site5293Zn2+ 1 (UniProtKB | ChEBI)
Binding site5296Zn2+ 1 (UniProtKB | ChEBI)
Binding site5304Zn2+ 2 (UniProtKB | ChEBI)
Binding site5307Zn2+ 2 (UniProtKB | ChEBI)
Binding site5314Zn2+ 1 (UniProtKB | ChEBI)
Binding site5317Zn2+ 1 (UniProtKB | ChEBI)
Binding site5321Zn2+ 2 (UniProtKB | ChEBI)
Binding site5327Zn2+ 2 (UniProtKB | ChEBI)
Binding site5336Zn2+ 3 (UniProtKB | ChEBI)
Binding site5338Zn2+ 3 (UniProtKB | ChEBI)
Binding site5359Zn2+ 3 (UniProtKB | ChEBI)
Binding site5362Zn2+ 3 (UniProtKB | ChEBI)
Site5844-5845Cleavage; by 3C-like serine proteinase
Active site5860
Active site5862
Active site5961
Binding site6025Zn2+ 4 (UniProtKB | ChEBI)
Binding site6029Zn2+ 4 (UniProtKB | ChEBI)
Binding site6033Zn2+ 4 (UniProtKB | ChEBI)
Active site6037
Active site6042
Binding site6048Zn2+ 4 (UniProtKB | ChEBI)
Site6174-6175Cleavage; by 3C-like serine proteinase
Site6318-6319Cleavage; by 3C-like serine proteinase
Active site6363
Active site6380
Active site6412
Site6468-6469Cleavage; by 3C-like serine proteinase
Active site6509
Active site6585
Active site6613
Active site6647

GO annotations

AspectTerm
Cellular Componenthost cell membrane
Cellular Componentmembrane
Molecular Function3'-5'-RNA exonuclease activity
Molecular Function5'-3' DNA helicase activity
Molecular FunctionATP binding
Molecular FunctionATP hydrolysis activity
Molecular Functioncysteine-type peptidase activity
Molecular Functionendonuclease activity
Molecular FunctionmRNA (nucleoside-2'-O-)-methyltransferase activity
Molecular FunctionRNA binding
Molecular FunctionRNA helicase activity
Molecular FunctionRNA-dependent RNA polymerase activity
Molecular Functionserine-type peptidase activity
Molecular Functionzinc ion binding
Biological ProcessDNA-templated transcription
Biological Processmethylation
Biological Processproteolysis
Biological Processviral RNA genome replication
Biological Processviral translational frameshifting

Keywords

Protein family/group databases

Names & Taxonomy

Protein names

Gene names

    • Name
      rep
    • ORF names
      1a-1b

Organism names

  • Taxonomic identifier
  • Strain
    • GC32
  • Taxonomic lineage
    Viruses > Riboviria > Orthornavirae > Pisuviricota > Pisoniviricetes > Nidovirales > Tornidovirineae > Tobaniviridae > Torovirinae > Torovirus > Renitovirus > Bovine torovirus

Accessions

  • Primary accession
    P0C6V8
  • Secondary accessions
    • Q3T8J1
    • Q3T8J2
    • Q9YP98

Proteomes

Subcellular Location

Putative papain-like proteinase

Host membrane
; Multi-pass membrane protein

Non-structural protein 2

Host membrane
; Multi-pass membrane protein

Non-structural protein 4

Host membrane
; Multi-pass membrane protein

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane2191-2211Helical
Transmembrane2219-2239Helical
Transmembrane2266-2286Helical
Transmembrane2411-2431Helical
Transmembrane2521-2541Helical
Transmembrane2546-2566Helical
Transmembrane2769-2789Helical
Transmembrane2937-2957Helical
Transmembrane2986-3006Helical
Transmembrane3022-3042Helical
Transmembrane3422-3442Helical
Transmembrane3456-3478Helical
Transmembrane3486-3506Helical
Transmembrane3514-3534Helical
Transmembrane3538-3558Helical
Transmembrane3573-3593Helical
Transmembrane3598-3613Helical

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00002838591-2753Putative papain-like proteinase
ChainPRO_00002838602754-3131Non-structural protein 2
ChainPRO_00002838623132-34183C-like serine proteinase
ChainPRO_00002838633419-3677Non-structural protein 4
ChainPRO_00002838643678-3854Non-structural protein 5
ChainPRO_00002838653855-4036Non-structural protein 6
ChainPRO_00002838664037-4121Non-structural protein 7
ChainPRO_00002838674122-4274Non-structural protein 8
ChainPRO_00002838684275-5286RNA-directed RNA polymerase
ChainPRO_00002838695287-5844Helicase
ChainPRO_00002838705845-6174Exoribonuclease
ChainPRO_00002838716175-6318Non-structural protein 13
ChainPRO_00002838726319-6468Uridylate-specific endoribonuclease
ChainPRO_00002838736469-6733Putative 2'-O-methyl transferase

Post-translational modification

Isoform Replicase polyprotein 1ab

Specific enzymatic cleavages in vivo by its own protease yield mature proteins. 3CL-PRO is autocatalytically processed.

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain1633-1814Macro
Region2183-2565HD1
Region2769-3042HD2
Region3430-3613HD3
Domain4442-4673NiRAN
Domain4981-5132RdRp catalytic
Domain5289-5404CV ZBD
Domain5509-5688+RNA virus helicase ATP-binding
Domain5689-5848+RNA virus helicase C-terminal
Domain5846-6059ExoN
Domain6327-6467NendoU
Domain6469-6733Nidovirus-type SAM-dependent 2'-O-MTase

Domain

The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane.

Keywords

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete

This entry describes 2 isoforms produced by Ribosomal frameshifting.

P0C6V8-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Name
    Replicase polyprotein 1ab
  • Synonyms
    pp1ab
  • Note
    Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Length
    6,733
  • Mass (Da)
    765,975
  • Last updated
    2008-06-10 v1
  • Checksum
    7947CE8F5F1FA90D

P0C6F4-1

The sequence of this isoform can be found in the external entry linked below. Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

View isoform
  • Name
    Replicase polyprotein 1a
  • Synonyms
    pp1a, ORF1a polyprotein
  • Note
    Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.
  • See also
    sequence in UniParc or sequence clusters in UniRef

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AY427798
EMBL· GenBank· DDBJ
AAS17963.1
EMBL· GenBank· DDBJ
Genomic RNA
U50390
EMBL· GenBank· DDBJ
AAD11480.1
EMBL· GenBank· DDBJ
mRNA

Similar Proteins

Disclaimer

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