P0C587 · HEM1_ORYSJ
- ProteinGlutamyl-tRNA reductase, chloroplastic
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids537 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score3/5
Function
function
Catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA).
Miscellaneous
During catalysis, the active site Cys acts as a nucleophile attacking the alpha-carbonyl group of tRNA-bound glutamate with the formation of a thioester intermediate between enzyme and glutamate, and the concomitant release of tRNA(Glu). The thioester intermediate is finally reduced by direct hydride transfer from NADPH, to form the product GSA (By similarity).
Catalytic activity
- (S)-4-amino-5-oxopentanoate + NADP+ + tRNA(Glu) = H+ + L-glutamyl-tRNA(Glu) + NADPH
Pathway
Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 1/2.
Features
Showing features for binding site, active site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 134-137 | substrate | ||||
Sequence: TCNR | ||||||
Active site | 135 | Nucleophile | ||||
Sequence: C | ||||||
Site | 184 | Important for activity | ||||
Sequence: H | ||||||
Binding site | 194 | substrate | ||||
Sequence: S | ||||||
Binding site | 199-201 | substrate | ||||
Sequence: EGQ | ||||||
Binding site | 205 | substrate | ||||
Sequence: Q | ||||||
Binding site | 276-281 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: GAGKMG |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chloroplast | |
Molecular Function | glutamyl-tRNA reductase activity | |
Molecular Function | NADP binding | |
Biological Process | chlorophyll biosynthetic process | |
Biological Process | protoporphyrinogen IX biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameGlutamyl-tRNA reductase, chloroplastic
- EC number
- Short namesGluTR
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > BOP clade > Oryzoideae > Oryzeae > Oryzinae > Oryza > Oryza sativa
Accessions
- Primary accessionP0C587
- Secondary accessions
Proteomes
Genome annotation databases
Subcellular Location
PTM/Processing
Features
Showing features for transit peptide, chain, modified residue (large scale data).
Type | ID | Position(s) | Source | Description | |||
---|---|---|---|---|---|---|---|
Transit peptide | 1-48 | UniProt | Chloroplast | ||||
Sequence: MMASTTSATAAGGAFAAAKTRAGSSAAGGGACARVAAGGRRRSGVVVR | |||||||
Chain | PRO_0000013313 | 49-537 | UniProt | Glutamyl-tRNA reductase, chloroplastic | |||
Sequence: CDAGVEAQAQAQAVAKAASVAALEQFKISADRYMKERSSIAVIGLSVHTAPVEMREKLAVAEELWPRAISELTSLNHIEEAAVLSTCNRMEIYVVALSWNRGIREVVDWMSKKSGIPASELREHLFMLRDSDATRHLFEVSAGLDSLVLGEGQILAQVKQVVRSGQNSGGLGKNIDRMFKDAITAGKRVRCETNISSGAVSVSSAAVELALMKLPKSECLSARMLLIGAGKMGKLVVKHLIAKGCKKVVVVNRSVERVDAIREEMKDIEIVYRPLTEMYEAAAEADVVFTSTASETPLFTKEHAEALPAISDAMGGVRLFVDISVPRNVSACVSEVGHARVYNVDDLKEVVEANKEDRLRKAMEAQTIITQELKRFEAWRDSLETVPTIKKLRSYADRIRASELEKCLQKIGEDALTKKMRRSIEELSTGIVNKLLHGPLQHLRCDGSDSRTLDETLENMHALNRMFSLDTEKAIIEQKIKAKVEKSQN | |||||||
Modified residue (large scale data) | 516 | PTMeXchange | Phosphoserine | ||||
Sequence: S |
Proteomic databases
Expression
Gene expression databases
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length537
- Mass (Da)58,419
- Last updated2007-07-24 v1
- Checksum51415C5525E3C640
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A0N7KS13 | A0A0N7KS13_ORYSJ | Os10g0502400 | 272 |
Sequence caution
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 346 | in Ref. 5; AK099393 | ||||
Sequence: L → F |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AC078840 EMBL· GenBank· DDBJ | AAG13620.1 EMBL· GenBank· DDBJ | Genomic DNA | Different initiation | |
AC079888 EMBL· GenBank· DDBJ | AAM93670.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DP000086 EMBL· GenBank· DDBJ | ABB47844.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP008216 EMBL· GenBank· DDBJ | BAF26905.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP014966 EMBL· GenBank· DDBJ | BAT11537.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AK099393 EMBL· GenBank· DDBJ | - | mRNA | No translation available. |