P0C1V7 · CA1AB_CONAN
- ProteinAlpha-conotoxin AnIB
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Alpha-conotoxin AnIB
Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them. This synthetic toxin blocks rat alpha-3beta-2/CHRNA3-CHRNB2 nAChRs (IC50=0.3 nM) and alpha-7/CHRNA7 nAChRs (IC50=76 nM).
Alpha-conotoxin AnIA
This synthetic toxin blocks rat alpha-3-beta-2/CHRNA3-CHRNB2 nAChRs (IC50=0.2 nM).
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Aspect | Term | |
---|---|---|
Cellular Component | extracellular region | |
Cellular Component | host cell postsynaptic membrane | |
Molecular Function | acetylcholine receptor inhibitor activity | |
Molecular Function | ion channel regulator activity | |
Molecular Function | toxin activity |
Keywords
- Molecular function
Names & Taxonomy
Protein names
- Recommended nameAlpha-conotoxin AnIB
- Cleaved into 1 chains
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Spiralia > Lophotrochozoa > Mollusca > Gastropoda > Caenogastropoda > Neogastropoda > Conoidea > Conidae > Conus > Floraconus
Accessions
- Primary accessionP0C1V7
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 1 | 1.4-fold increase in inhibitory potency on alpha-3-beta-2/CHRNA3-CHRNB2. | ||||
Sequence: G → GG | ||||||
Mutagenesis | 1 | 1.6-fold increase in inhibitory potency on alpha-3-beta-2/CHRNA3-CHRNB2. | ||||
Sequence: Missing |
PTM/Processing
Features
Showing features for peptide, disulfide bond, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Peptide | PRO_0000249780 | 1-17 | Alpha-conotoxin AnIB | |||
Sequence: GGCCSHPACAANNQDYC | ||||||
Disulfide bond | 3↔9 | |||||
Sequence: CCSHPAC | ||||||
Peptide | PRO_0000249781 | 3-17 | Alpha-conotoxin AnIA | |||
Sequence: CCSHPACAANNQDYC | ||||||
Disulfide bond | 4↔17 | |||||
Sequence: CSHPACAANNQDYC | ||||||
Modified residue | 16 | Sulfotyrosine | ||||
Sequence: Y | ||||||
Modified residue | 17 | Cysteine amide | ||||
Sequence: C |
Post-translational modification
C-terminal amidation, in synergy with sulfation, is important for activity and structure stability (PubMed:14971903, PubMed:34671739).
Non-amidated conotoxins has a 1.9-fold lower inhibitory potency on alpha-3-beta-2/CHRNA3-CHRNB2 nAChRs (IC50=0.54 nM), and a 4.8-fold lower inhibitory potency on alpha-7/CHRNA7 nAChRs (IC50=367 nM), when compared to sulfated conotoxin (PubMed:14971903).
Non-amidated conotoxins has a 1.9-fold lower inhibitory potency on alpha-3-beta-2/CHRNA3-CHRNB2 nAChRs (IC50=0.54 nM), and a 4.8-fold lower inhibitory potency on alpha-7/CHRNA7 nAChRs (IC50=367 nM), when compared to sulfated conotoxin (PubMed:14971903).
Sulfation at Tyr-16, in synergy with amidation, is important for activity and structure stability (PubMed:14971903, PubMed:34671739).
Non-sulfated conotoxin has a 2.3-fold lower inhibitory potency on alpha-3-beta-2/CHRNA3-CHRNB2 nAChRs (IC50=0.64 nM), and a 11-fold lower inhibitory potency on alpha-7/CHRNA7 nAChRs (IC50=836 nM), when compared to sulfated conotoxin (PubMed:14971903).
Non-sulfated conotoxin has a 2.3-fold lower inhibitory potency on alpha-3-beta-2/CHRNA3-CHRNB2 nAChRs (IC50=0.64 nM), and a 11-fold lower inhibitory potency on alpha-7/CHRNA7 nAChRs (IC50=836 nM), when compared to sulfated conotoxin (PubMed:14971903).
Keywords
- PTM
Expression
Tissue specificity
Expressed by the venom duct.
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 5-7 | Ser-Xaa-Pro motif, crucial for potent interaction with nAChR | ||||
Sequence: SHP |
Domain
The cysteine framework is I (CC-C-C). Alpha4/7 pattern.
Sequence similarities
Belongs to the conotoxin A superfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length17
- Mass (Da)1,714
- Last updated2006-09-19 v1
- Checksum76F84AFDFAC99005
Mass Spectrometry
Alpha-conotoxin AnIB
Molecular mass is 1,787.4 Da. Determined by Electrospray.Alpha-conotoxin AnIA
Molecular mass is 1,673.4 Da. Determined by Electrospray.Keywords
- Technical term