P0C1V7 · CA1AB_CONAN

Function

function

Alpha-conotoxin AnIB

Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them. This synthetic toxin blocks rat alpha-3beta-2/CHRNA3-CHRNB2 nAChRs (IC50=0.3 nM) and alpha-7/CHRNA7 nAChRs (IC50=76 nM).

Alpha-conotoxin AnIA

This synthetic toxin blocks rat alpha-3-beta-2/CHRNA3-CHRNB2 nAChRs (IC50=0.2 nM).

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentextracellular region
Cellular Componenthost cell postsynaptic membrane
Molecular Functionacetylcholine receptor inhibitor activity
Molecular Functionion channel regulator activity
Molecular Functiontoxin activity

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Alpha-conotoxin AnIB
  • Cleaved into 1 chains

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Spiralia > Lophotrochozoa > Mollusca > Gastropoda > Caenogastropoda > Neogastropoda > Conoidea > Conidae > Conus > Floraconus

Accessions

  • Primary accession
    P0C1V7

Organism-specific databases

Subcellular Location

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis11.4-fold increase in inhibitory potency on alpha-3-beta-2/CHRNA3-CHRNB2.
Mutagenesis11.6-fold increase in inhibitory potency on alpha-3-beta-2/CHRNA3-CHRNB2.

PTM/Processing

Features

Showing features for peptide, disulfide bond, modified residue.

TypeIDPosition(s)Description
PeptidePRO_00002497801-17Alpha-conotoxin AnIB
Disulfide bond3↔9
PeptidePRO_00002497813-17Alpha-conotoxin AnIA
Disulfide bond4↔17
Modified residue16Sulfotyrosine
Modified residue17Cysteine amide

Post-translational modification

C-terminal amidation, in synergy with sulfation, is important for activity and structure stability (PubMed:14971903, PubMed:34671739).
Non-amidated conotoxins has a 1.9-fold lower inhibitory potency on alpha-3-beta-2/CHRNA3-CHRNB2 nAChRs (IC50=0.54 nM), and a 4.8-fold lower inhibitory potency on alpha-7/CHRNA7 nAChRs (IC50=367 nM), when compared to sulfated conotoxin (PubMed:14971903).
Sulfation at Tyr-16, in synergy with amidation, is important for activity and structure stability (PubMed:14971903, PubMed:34671739).
Non-sulfated conotoxin has a 2.3-fold lower inhibitory potency on alpha-3-beta-2/CHRNA3-CHRNB2 nAChRs (IC50=0.64 nM), and a 11-fold lower inhibitory potency on alpha-7/CHRNA7 nAChRs (IC50=836 nM), when compared to sulfated conotoxin (PubMed:14971903).

Keywords

Expression

Tissue specificity

Expressed by the venom duct.

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region5-7Ser-Xaa-Pro motif, crucial for potent interaction with nAChR

Domain

The cysteine framework is I (CC-C-C). Alpha4/7 pattern.

Sequence similarities

Belongs to the conotoxin A superfamily.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    17
  • Mass (Da)
    1,714
  • Last updated
    2006-09-19 v1
  • Checksum
    76F84AFDFAC99005
GGCCSHPACAANNQDYC

Mass Spectrometry

Alpha-conotoxin AnIB

Molecular mass is 1,787.4 Da. Determined by Electrospray.

Alpha-conotoxin AnIA

Molecular mass is 1,673.4 Da. Determined by Electrospray.

Keywords

Similar Proteins

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