P0C1A8 · PMEA_DICCH
- ProteinPectinesterase A
- GenepemA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids366 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Involved in maceration and soft-rotting of plant tissue.
Catalytic activity
- [(1->4)-alpha-D-galacturonosyl methyl ester](n) + n H2O = [(1->4)-alpha-D-galacturonosyl](n) + n methanol + n H+
[(1→4)-α-D-galacturonosyl methyl ester](n) RHEA-COMP:14573
+ n CHEBI:15377 = [(1→4)-α-D-galacturonosyl](n) RHEA-COMP:14570
+ n CHEBI:17790 + n CHEBI:15378
Pathway
Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.
Features
Showing features for binding site, site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 109 | substrate | ||||
Sequence: T | ||||||
Binding site | 153 | substrate | ||||
Sequence: Q | ||||||
Site | 177 | Transition state stabilizer | ||||
Sequence: Q | ||||||
Active site | 178 | Proton donor | ||||
Sequence: D | ||||||
Active site | 199 | Nucleophile | ||||
Sequence: D | ||||||
Binding site | 219 | substrate | ||||
Sequence: R | ||||||
Binding site | 226 | substrate | ||||
Sequence: N | ||||||
Binding site | 230 | substrate | ||||
Sequence: Y | ||||||
Binding site | 267 | substrate | ||||
Sequence: R | ||||||
Binding site | 269 | substrate | ||||
Sequence: W | ||||||
Binding site | 272 | substrate | ||||
Sequence: T |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cell outer membrane | |
Cellular Component | extracellular region | |
Molecular Function | pectinesterase activity | |
Biological Process | cell wall modification | |
Biological Process | pectin catabolic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePectinesterase A
- EC number
- Short namesPE A
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Pectobacteriaceae > Dickeya
Accessions
- Primary accessionP0C1A8
- Secondary accessions
Subcellular Location
PTM/Processing
Features
Showing features for signal, chain, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-24 | |||||
Sequence: MLKTISGTLALSLIIAASVHQAQA | ||||||
Chain | PRO_0000023498 | 25-366 | Pectinesterase A | |||
Sequence: ATTYNAVVSKSSSDGKTFKTIADAIASAPAGSTPFVILIKNGVYNERLTITRNNLLLKGESRNGAVIAAATAAGTLKSDGSKWGTAGSSTITISAKDFSAQSLTIRNDFDFPANQAKSDSDSSKIKDTQAVALYVTKSGDRAYFKDVSLVGYQDTLYVSGGRSFFSDCRISGTVDFIFGDGTALFNNCDLVSRYRADVKSGNVSGYLTAPSTNINQKYGLVITNSRVIRESDSVPAKSYGLGRPWHPTTTFSDGRYADPNAIGQTVFLNTSMDNHIYGWDKMSGKDKNGNTIWFNPEDSRFFEYKSYGAGAAVSKDRRQLTDAQAAEYTQSKVLGDWTPTLP | ||||||
Disulfide bond | 192↔212 | |||||
Sequence: CRISGTVDFIFGDGTALFNNC |
Keywords
- PTM
Interaction
Subunit
Monomer.
Structure
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length366
- Mass (Da)39,319
- Last updated2006-04-18 v1
- ChecksumDA7DD9A45290660B
Keywords
- Technical term