P0C0S6 · H2AZ_MOUSE

  • Protein
    Histone H2A.Z
  • Gene
    H2az1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division. Essential for early development.

Miscellaneous

Down-regulation of H2az1 by RNA interference leads to death at early embryonic stages.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componenteuchromatin
Cellular Componentheterochromatin
Cellular Componentnucleosome
Cellular Componentnucleus
Molecular Functionnucleosomal DNA binding
Molecular Functionprotein heterodimerization activity
Molecular FunctionRNA polymerase II cis-regulatory region sequence-specific DNA binding
Molecular FunctionRNA polymerase II core promoter sequence-specific DNA binding
Molecular Functionstructural constituent of chromatin
Biological Processcellular response to estradiol stimulus
Biological Processcellular response to insulin stimulus
Biological Processchromatin organization
Biological Processheterochromatin formation
Biological Processpositive regulation of transcription by RNA polymerase II

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Histone H2A.Z
  • Short names
    H2A/z

Gene names

    • Name
      H2az1
    • Synonyms
      H2afz
      , H2az

Organism names

  • Taxonomic identifier
  • Strains
    • C57BL/6J
    • C57BL/6 X CBA
    • BALB/cJ
    • DBA/2J
    • NOD
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    P0C0S6
  • Secondary accessions
    • B2RXZ3
    • P17317
    • Q3UA55
    • Q3UK43
    • Q68FD2

Proteomes

Organism-specific databases

Subcellular Location

Nucleus
Chromosome
Note: Enriched in constitutive heterochromatin (PubMed:12660166, PubMed:15195148).
Absent from facultative heterochromatin of the inactive X chromosome (PubMed:12660166).

Keywords

PTM/Processing

Features

Showing features for initiator methionine, chain, modified residue, cross-link.

TypeIDPosition(s)Description
Initiator methionine1Removed
ChainPRO_00000552982-128Histone H2A.Z
Modified residue5N6-acetyllysine; alternate
Modified residue5N6-methyllysine; alternate
Modified residue8N6-acetyllysine; alternate
Modified residue8N6-methyllysine; alternate
Modified residue12N6-acetyllysine; alternate
Modified residue12N6-lactoyllysine; alternate
Modified residue14N6-acetyllysine; alternate
Modified residue14N6-lactoyllysine; alternate
Modified residue116N6-lactoyllysine
Cross-link122Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Post-translational modification

Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression.
Acetylated on Lys-5, Lys-8, Lys-12 and Lys-14 by KAT2A; KAT2A is recruited by the XPC complex in absence of DNA damage (By similarity).
Acetylated on Lys-5, Lys-8 and Lys-12 during interphase; acetylation disappears at mitosis (PubMed:16204459, PubMed:7217105).
Acetylation by the NuA4 histone acetyltransferase complex is required for hematopoietic stem cell maintenance (PubMed:32542325).
Not phosphorylated.
Monomethylated on Lys-5 and Lys-8 by SETD6. SETD6 predominantly methylates Lys-8, lys-5 being a possible secondary site.
Lactylated in macrophages by EP300/P300 by using lactoyl-CoA directly derived from endogenous or exogenous lactate, leading to stimulates gene transcription.

Keywords

Proteomic databases

PTM databases

Expression

Gene expression databases

Interaction

Subunit

Interacts (via M6 cassette) with ANP32E; leading to removal of H2A.Z/H2AZ1 from the nucleosome (By similarity).
The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. H2A or its variant H2AZ1 forms a heterodimer with H2B (By similarity).
Interacts with INCENP (PubMed:12660166).
Interacts with VPS72 (via N-terminal domain); the interaction is enhanced by VPS72 phosphorylation which is promoted by ZNHIT1 (PubMed:30842416).
Interacts with PWWP2A (By similarity).
Interacts with FH (when phosphorylated by PRKDC) (By similarity).
Interacts with ZNHIT1; the interaction results in recruitment of H2AZ1 to the MYOG promoter region which is required for muscle-specific gene expression (By similarity).
View interactors in UniProtKB
View CPX-5715 in Complex Portal

Protein-protein interaction databases

Miscellaneous

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region1-17Required for interaction with INCENP
Region1-25Disordered
Region89-100M6 cassette
Region93-103Required for interaction with INCENP
Region120-128Required for interaction with PWWP2A

Sequence similarities

Belongs to the histone H2A family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    128
  • Mass (Da)
    13,553
  • Last updated
    2007-01-23 v2
  • Checksum
    E024E53818230371
MAGGKAGKDSGKAKTKAVSRSQRAGLQFPVGRIHRHLKSRTTSHGRVGATAAVYSAAILEYLTAEVLELAGNASKDLKVKRITPRHLQLAIRGDEELDSLIKATIAGGGVIPHIHKSLIGKKGQQKTV

Computationally mapped potential isoform sequences

There are 3 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
Q3UA95Q3UA95_MOUSEH2az1105
G3UWL7G3UWL7_MOUSEH2az187
G3UX40G3UX40_MOUSEH2az154

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict12in Ref. 3; BAE30460
Sequence conflict29in Ref. 3; BAE26959

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U70494
EMBL· GenBank· DDBJ
AAB09578.1
EMBL· GenBank· DDBJ
mRNA
AY074806
EMBL· GenBank· DDBJ
AAL71864.1
EMBL· GenBank· DDBJ
mRNA
AK028176
EMBL· GenBank· DDBJ
BAC25791.1
EMBL· GenBank· DDBJ
mRNA
AK088704
EMBL· GenBank· DDBJ
BAC40515.1
EMBL· GenBank· DDBJ
mRNA
AK143572
EMBL· GenBank· DDBJ
BAE25443.1
EMBL· GenBank· DDBJ
mRNA
AK146181
EMBL· GenBank· DDBJ
BAE26959.1
EMBL· GenBank· DDBJ
mRNA
AK150454
EMBL· GenBank· DDBJ
BAE29574.1
EMBL· GenBank· DDBJ
mRNA
AK151052
EMBL· GenBank· DDBJ
BAE30069.1
EMBL· GenBank· DDBJ
mRNA
AK151509
EMBL· GenBank· DDBJ
BAE30460.1
EMBL· GenBank· DDBJ
mRNA
AK151868
EMBL· GenBank· DDBJ
BAE30756.1
EMBL· GenBank· DDBJ
mRNA
AK152398
EMBL· GenBank· DDBJ
BAE31186.1
EMBL· GenBank· DDBJ
mRNA
AK152848
EMBL· GenBank· DDBJ
BAE31541.1
EMBL· GenBank· DDBJ
mRNA
AK168178
EMBL· GenBank· DDBJ
BAE40138.1
EMBL· GenBank· DDBJ
mRNA
BC079903
EMBL· GenBank· DDBJ
AAH79903.1
EMBL· GenBank· DDBJ
mRNA
BC147478
EMBL· GenBank· DDBJ
AAI47479.1
EMBL· GenBank· DDBJ
mRNA
BC158035
EMBL· GenBank· DDBJ
AAI58036.1
EMBL· GenBank· DDBJ
mRNA
BC171999
EMBL· GenBank· DDBJ
AAI71999.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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