P0C0S6 · H2AZ_MOUSE
- ProteinHistone H2A.Z
- GeneH2az1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids128 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division. Essential for early development.
Miscellaneous
Down-regulation of H2az1 by RNA interference leads to death at early embryonic stages.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | euchromatin | |
Cellular Component | heterochromatin | |
Cellular Component | nucleosome | |
Cellular Component | nucleus | |
Molecular Function | nucleosomal DNA binding | |
Molecular Function | protein heterodimerization activity | |
Molecular Function | RNA polymerase II cis-regulatory region sequence-specific DNA binding | |
Molecular Function | RNA polymerase II core promoter sequence-specific DNA binding | |
Molecular Function | structural constituent of chromatin | |
Biological Process | cellular response to estradiol stimulus | |
Biological Process | cellular response to insulin stimulus | |
Biological Process | chromatin organization | |
Biological Process | heterochromatin formation | |
Biological Process | positive regulation of transcription by RNA polymerase II |
Keywords
- Molecular function
Names & Taxonomy
Protein names
- Recommended nameHistone H2A.Z
- Short namesH2A/z
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionP0C0S6
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Keywords
- Cellular component
PTM/Processing
Features
Showing features for initiator methionine, chain, modified residue, cross-link.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Initiator methionine | 1 | Removed | ||||
Sequence: M | ||||||
Chain | PRO_0000055298 | 2-128 | Histone H2A.Z | |||
Sequence: AGGKAGKDSGKAKTKAVSRSQRAGLQFPVGRIHRHLKSRTTSHGRVGATAAVYSAAILEYLTAEVLELAGNASKDLKVKRITPRHLQLAIRGDEELDSLIKATIAGGGVIPHIHKSLIGKKGQQKTV | ||||||
Modified residue | 5 | N6-acetyllysine; alternate | ||||
Sequence: K | ||||||
Modified residue | 5 | N6-methyllysine; alternate | ||||
Sequence: K | ||||||
Modified residue | 8 | N6-acetyllysine; alternate | ||||
Sequence: K | ||||||
Modified residue | 8 | N6-methyllysine; alternate | ||||
Sequence: K | ||||||
Modified residue | 12 | N6-acetyllysine; alternate | ||||
Sequence: K | ||||||
Modified residue | 12 | N6-lactoyllysine; alternate | ||||
Sequence: K | ||||||
Modified residue | 14 | N6-acetyllysine; alternate | ||||
Sequence: K | ||||||
Modified residue | 14 | N6-lactoyllysine; alternate | ||||
Sequence: K | ||||||
Modified residue | 116 | N6-lactoyllysine | ||||
Sequence: K | ||||||
Cross-link | 122 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) | ||||
Sequence: K |
Post-translational modification
Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression.
Acetylated on Lys-5, Lys-8, Lys-12 and Lys-14 by KAT2A; KAT2A is recruited by the XPC complex in absence of DNA damage (By similarity).
Acetylated on Lys-5, Lys-8 and Lys-12 during interphase; acetylation disappears at mitosis (PubMed:16204459, PubMed:7217105).
Acetylation by the NuA4 histone acetyltransferase complex is required for hematopoietic stem cell maintenance (PubMed:32542325).
Acetylated on Lys-5, Lys-8 and Lys-12 during interphase; acetylation disappears at mitosis (PubMed:16204459, PubMed:7217105).
Acetylation by the NuA4 histone acetyltransferase complex is required for hematopoietic stem cell maintenance (PubMed:32542325).
Not phosphorylated.
Monomethylated on Lys-5 and Lys-8 by SETD6. SETD6 predominantly methylates Lys-8, lys-5 being a possible secondary site.
Lactylated in macrophages by EP300/P300 by using lactoyl-CoA directly derived from endogenous or exogenous lactate, leading to stimulates gene transcription.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Gene expression databases
Interaction
Subunit
Interacts (via M6 cassette) with ANP32E; leading to removal of H2A.Z/H2AZ1 from the nucleosome (By similarity).
The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. H2A or its variant H2AZ1 forms a heterodimer with H2B (By similarity).
Interacts with INCENP (PubMed:12660166).
Interacts with VPS72 (via N-terminal domain); the interaction is enhanced by VPS72 phosphorylation which is promoted by ZNHIT1 (PubMed:30842416).
Interacts with PWWP2A (By similarity).
Interacts with FH (when phosphorylated by PRKDC) (By similarity).
Interacts with ZNHIT1; the interaction results in recruitment of H2AZ1 to the MYOG promoter region which is required for muscle-specific gene expression (By similarity).
The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. H2A or its variant H2AZ1 forms a heterodimer with H2B (By similarity).
Interacts with INCENP (PubMed:12660166).
Interacts with VPS72 (via N-terminal domain); the interaction is enhanced by VPS72 phosphorylation which is promoted by ZNHIT1 (PubMed:30842416).
Interacts with PWWP2A (By similarity).
Interacts with FH (when phosphorylated by PRKDC) (By similarity).
Interacts with ZNHIT1; the interaction results in recruitment of H2AZ1 to the MYOG promoter region which is required for muscle-specific gene expression (By similarity).
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-17 | Required for interaction with INCENP | ||||
Sequence: MAGGKAGKDSGKAKTKA | ||||||
Region | 1-25 | Disordered | ||||
Sequence: MAGGKAGKDSGKAKTKAVSRSQRAG | ||||||
Region | 89-100 | M6 cassette | ||||
Sequence: LAIRGDEELDSL | ||||||
Region | 93-103 | Required for interaction with INCENP | ||||
Sequence: GDEELDSLIKA | ||||||
Region | 120-128 | Required for interaction with PWWP2A | ||||
Sequence: GKKGQQKTV |
Sequence similarities
Belongs to the histone H2A family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length128
- Mass (Da)13,553
- Last updated2007-01-23 v2
- ChecksumE024E53818230371
Computationally mapped potential isoform sequences
There are 3 potential isoforms mapped to this entry
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 12 | in Ref. 3; BAE30460 | ||||
Sequence: K → E | ||||||
Sequence conflict | 29 | in Ref. 3; BAE26959 | ||||
Sequence: P → A |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U70494 EMBL· GenBank· DDBJ | AAB09578.1 EMBL· GenBank· DDBJ | mRNA | ||
AY074806 EMBL· GenBank· DDBJ | AAL71864.1 EMBL· GenBank· DDBJ | mRNA | ||
AK028176 EMBL· GenBank· DDBJ | BAC25791.1 EMBL· GenBank· DDBJ | mRNA | ||
AK088704 EMBL· GenBank· DDBJ | BAC40515.1 EMBL· GenBank· DDBJ | mRNA | ||
AK143572 EMBL· GenBank· DDBJ | BAE25443.1 EMBL· GenBank· DDBJ | mRNA | ||
AK146181 EMBL· GenBank· DDBJ | BAE26959.1 EMBL· GenBank· DDBJ | mRNA | ||
AK150454 EMBL· GenBank· DDBJ | BAE29574.1 EMBL· GenBank· DDBJ | mRNA | ||
AK151052 EMBL· GenBank· DDBJ | BAE30069.1 EMBL· GenBank· DDBJ | mRNA | ||
AK151509 EMBL· GenBank· DDBJ | BAE30460.1 EMBL· GenBank· DDBJ | mRNA | ||
AK151868 EMBL· GenBank· DDBJ | BAE30756.1 EMBL· GenBank· DDBJ | mRNA | ||
AK152398 EMBL· GenBank· DDBJ | BAE31186.1 EMBL· GenBank· DDBJ | mRNA | ||
AK152848 EMBL· GenBank· DDBJ | BAE31541.1 EMBL· GenBank· DDBJ | mRNA | ||
AK168178 EMBL· GenBank· DDBJ | BAE40138.1 EMBL· GenBank· DDBJ | mRNA | ||
BC079903 EMBL· GenBank· DDBJ | AAH79903.1 EMBL· GenBank· DDBJ | mRNA | ||
BC147478 EMBL· GenBank· DDBJ | AAI47479.1 EMBL· GenBank· DDBJ | mRNA | ||
BC158035 EMBL· GenBank· DDBJ | AAI58036.1 EMBL· GenBank· DDBJ | mRNA | ||
BC171999 EMBL· GenBank· DDBJ | AAI71999.1 EMBL· GenBank· DDBJ | mRNA |