P0AG96 · SECE_ECOLI
- ProteinProtein translocase subunit SecE
- GenesecE
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids127 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Essential subunit of the protein translocation channel SecYEG. Clamps together the 2 halves of SecY. May contact the channel plug during translocation. Overexpression of some hybrid proteins has been thought to jam the protein secretion apparatus resulting in cell death; while this may be true it also results in FtsH-mediated degradation of SecY.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Aspect | Term | |
---|---|---|
Cellular Component | cell envelope Sec protein transport complex | |
Cellular Component | membrane | |
Cellular Component | plasma membrane | |
Molecular Function | protein transmembrane transporter activity | |
Biological Process | intracellular protein transmembrane transport | |
Biological Process | intracellular protein transport | |
Biological Process | protein insertion into membrane from inner side | |
Biological Process | protein secretion | |
Biological Process | protein transport by the Sec complex | |
Biological Process | SRP-dependent cotranslational protein targeting to membrane, translocation |
Keywords
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameProtein translocase subunit SecE
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionP0AG96
- Secondary accessions
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Cell inner membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-19 | Cytoplasmic | ||||
Sequence: MSANTEAQGSGRGLEAMKW | ||||||
Transmembrane | 20-32 | Helical | ||||
Sequence: VVVVALLLVAIVG | ||||||
Topological domain | 33-48 | Periplasmic | ||||
Sequence: NYLYRDIMLPLRALAV | ||||||
Transmembrane | 49-60 | Helical | ||||
Sequence: VILIAAAGGVAL | ||||||
Topological domain | 61-97 | Cytoplasmic | ||||
Sequence: LTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIV | ||||||
Transmembrane | 98-115 | Helical | ||||
Sequence: AAVTAVMSLILWGLDGIL | ||||||
Topological domain | 116-127 | Periplasmic | ||||
Sequence: VRLVSFITGLRF |
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Essential; deletion experiments lead to loss of the SecYEG translocation channel. Leads to loss of translocation of lipoproteins Lpp and BRP (PubMed:15140892) and accumulation of signal peptidase I (lepB) in a soluble fraction (PubMed:21778229).
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 120 | Massive ion flux when cross-linked to SecYC-67 mutation. | ||||
Sequence: S → C |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000104163 | 1-127 | Protein translocase subunit SecE | |||
Sequence: MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLRALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTAVMSLILWGLDGILVRLVSFITGLRF |
Post-translational modification
Degraded by FtsH when the SecYEG complex is jammed or upon treatment with antibiotics that block translation elongation such as chloramphenicol.
Proteomic databases
Interaction
Subunit
Part of the essential Sec protein translocation complex which comprises SecA, SecYEG and auxiliary proteins SecDF-YajC and YidC/OxaA. Heterotrimer consisting of SecY, SecE and SecG subunits. The SecDF-YidC-YajC translocase forms a supercomplex with SecYEG, called the holo-translocon (HTL) (PubMed:27435098).
The stoichiometry of the super complex may be SecYEG:YidC:SecDF 4:3:1, YajC is in the reconstituted complex (with SecDF) but as no antibody is available it could not be quantified (PubMed:27435098).
The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. The channel interacts with SecA via subunit SecY. SecE might contact ribosomal protein L23 and/or L29 when the translocation complex is docked with the ribosome
The stoichiometry of the super complex may be SecYEG:YidC:SecDF 4:3:1, YajC is in the reconstituted complex (with SecDF) but as no antibody is available it could not be quantified (PubMed:27435098).
The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. The channel interacts with SecA via subunit SecY. SecE might contact ribosomal protein L23 and/or L29 when the translocation complex is docked with the ribosome
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P0AG96 | secA P10408 | 2 | EBI-6404267, EBI-543213 | |
BINARY | P0AG96 | secY P0AGA2 | 5 | EBI-6404267, EBI-761422 |
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length127
- Mass (Da)13,643
- Last updated2005-12-20 v1
- Checksum94D37280522875CE
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
M30610 EMBL· GenBank· DDBJ | AAA24621.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U00006 EMBL· GenBank· DDBJ | AAC43079.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U00096 EMBL· GenBank· DDBJ | AAC76955.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP009048 EMBL· GenBank· DDBJ | BAE77339.1 EMBL· GenBank· DDBJ | Genomic DNA |