P0AFE8 · NUOM_ECOLI
- ProteinNADH-quinone oxidoreductase subunit M
- GenenuoM
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids509 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.
Catalytic activity
- a quinone + NADH + 5 H+(in) = a quinol + NAD+ + 4 H+(out)
CHEBI:132124 + CHEBI:57945 + 5 H+ (in)CHEBI:15378= CHEBI:24646 + CHEBI:57540 + 4 H+ (out)CHEBI:15378
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | membrane | |
Cellular Component | NADH dehydrogenase complex | |
Cellular Component | plasma membrane | |
Cellular Component | respiratory chain complex I | |
Molecular Function | NADH dehydrogenase (ubiquinone) activity | |
Molecular Function | ubiquinone binding | |
Biological Process | aerobic respiration | |
Biological Process | ATP synthesis coupled electron transport | |
Biological Process | electron transport coupled proton transport | |
Biological Process | respiratory electron transport chain |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameNADH-quinone oxidoreductase subunit M
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionP0AFE8
- Secondary accessions
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Cell inner membrane ; Multi-pass membrane protein
Features
Showing features for transmembrane, topological domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 1-21 | Helical | ||||
Sequence: MLLPWLILIPFIGGFLCWQTE | ||||||
Topological domain | 22-29 | Cytoplasmic | ||||
Sequence: RFGVKVPR | ||||||
Transmembrane | 30-50 | Helical | ||||
Sequence: WIALITMGLTLALSLQLWLQG | ||||||
Topological domain | 51-82 | Periplasmic | ||||
Sequence: GYSLTQSAGIPQWQSEFDMPWIPRFGISIHLA | ||||||
Transmembrane | 83-103 | Helical | ||||
Sequence: IDGLSLLMVVLTGLLGVLAVL | ||||||
Topological domain | 104-121 | Cytoplasmic | ||||
Sequence: CSWKEIEKYQGFFHLNLM | ||||||
Transmembrane | 122-142 | Helical | ||||
Sequence: WILGGVIGVFLAIDMFLFFFF | ||||||
Topological domain | 143-173 | Periplasmic | ||||
Sequence: WEMMLVPMYFLIALWGHKASDGKTRITAATK | ||||||
Transmembrane | 174-194 | Helical | ||||
Sequence: FFIYTQASGLVMLIAILALVF | ||||||
Topological domain | 195-221 | Cytoplasmic | ||||
Sequence: VHYNATGVWTFNYEELLNTPMSSGVEY | ||||||
Transmembrane | 222-242 | Helical | ||||
Sequence: LLMLGFFIAFAVKMPVVPLHG | ||||||
Topological domain | 243-258 | Periplasmic | ||||
Sequence: WLPDAHSQAPTAGSVD | ||||||
Transmembrane | 259-279 | Helical | ||||
Sequence: LAGILLKTAAYGLLRFSLPLF | ||||||
Topological domain | 280-285 | Cytoplasmic | ||||
Sequence: PNASAE | ||||||
Transmembrane | 286-306 | Helical | ||||
Sequence: FAPIAMWLGVIGIFYGAWMAF | ||||||
Topological domain | 307-313 | Periplasmic | ||||
Sequence: AQTDIKR | ||||||
Transmembrane | 314-334 | Helical | ||||
Sequence: LIAYTSVSHMGFVLIAIYTGS | ||||||
Topological domain | 335-339 | Cytoplasmic | ||||
Sequence: QLAYQ | ||||||
Transmembrane | 340-360 | Helical | ||||
Sequence: GAVIQMIAHGLSAAGLFILCG | ||||||
Topological domain | 361-382 | Periplasmic | ||||
Sequence: QLYERIHTRDMRMMGGLWSKMK | ||||||
Transmembrane | 383-403 | Helical | ||||
Sequence: WLPALSLFFAVATLGMPGTGN | ||||||
Transmembrane | 404-424 | Helical | ||||
Sequence: FVGEFMILFGSFQVVPVITVI | ||||||
Topological domain | 425 | Periplasmic | ||||
Sequence: S | ||||||
Transmembrane | 426-446 | Helical | ||||
Sequence: TFGLVFASVYSLAMLHRAYFG | ||||||
Topological domain | 447-464 | Cytoplasmic | ||||
Sequence: KAKSQIASQELPGMSLRE | ||||||
Transmembrane | 465-485 | Helical | ||||
Sequence: LFMILLLVVLLVLLGFYPQPI | ||||||
Topological domain | 486-509 | Periplasmic | ||||
Sequence: LDTSHSAIGNIQQWFVNSVTTTRP |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000118039 | 1-509 | NADH-quinone oxidoreductase subunit M | |||
Sequence: MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSLQLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAVLCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHKASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSGVEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSLPLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGSQLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFAVATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKSQIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTRP |
Keywords
- PTM
Proteomic databases
Interaction
Subunit
Composed of 13 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex.
Complex viewer
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length509
- Mass (Da)56,525
- Last updated2005-12-20 v1
- Checksum3F8395683908EF97
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 464-465 | in Ref. 1 and 2 | ||||
Sequence: EL → DV |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X68301 EMBL· GenBank· DDBJ | CAA48372.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
L19568 EMBL· GenBank· DDBJ | AAA53583.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U00096 EMBL· GenBank· DDBJ | AAC75337.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP009048 EMBL· GenBank· DDBJ | BAA16105.1 EMBL· GenBank· DDBJ | Genomic DNA |