P0AF16 · MURJ_ECOLI
- ProteinLipid II flippase MurJ
- GenemurJ
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids511 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Involved in peptidoglycan biosynthesis. Transports lipid-linked peptidoglycan precursors from the inner to the outer leaflet of the cytoplasmic membrane.
Miscellaneous
FtsW (AC P0ABG4) is also proposed to act as a lipid II flippase. The identity of the lipid II flippase is controversial with conflicting in vivo and in vitro results.
Pathway
Cell wall biogenesis; peptidoglycan biosynthesis.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | division septum | |
Cellular Component | plasma membrane | |
Molecular Function | cardiolipin binding | |
Molecular Function | lipid-linked peptidoglycan transporter activity | |
Biological Process | cell wall organization | |
Biological Process | glycolipid translocation | |
Biological Process | lipid translocation | |
Biological Process | lipid-linked peptidoglycan transport | |
Biological Process | peptidoglycan biosynthetic process | |
Biological Process | regulation of cell shape |
Keywords
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameLipid II flippase MurJ
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionP0AF16
- Secondary accessions
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Cell inner membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-5 | Cytoplasmic | ||||
Sequence: MNLLK | ||||||
Transmembrane | 6-32 | Helical | ||||
Sequence: SLAAVSSMTMFSRVLGFARDAIVARIF | ||||||
Topological domain | 33-40 | Periplasmic | ||||
Sequence: GAGMATDA | ||||||
Transmembrane | 41-69 | Helical | ||||
Sequence: FFVAFKLPNLLRRIFAEGAFSQAFVPILA | ||||||
Topological domain | 70-82 | Cytoplasmic | ||||
Sequence: EYKSKQGEDATRV | ||||||
Transmembrane | 83-109 | Helical | ||||
Sequence: FVSYVSGLLTLALAVVTVAGMLAAPWV | ||||||
Topological domain | 110-123 | Periplasmic | ||||
Sequence: IMVTAPGFADTADK | ||||||
Transmembrane | 124-154 | Helical | ||||
Sequence: FALTSQLLKITFPYILLISLASLVGAILNTW | ||||||
Topological domain | 155-156 | Cytoplasmic | ||||
Sequence: NR | ||||||
Transmembrane | 157-178 | Helical | ||||
Sequence: FSIPAFAPTLLNISMIGFALFA | ||||||
Topological domain | 179-185 | Periplasmic | ||||
Sequence: APYFNPP | ||||||
Transmembrane | 186-207 | Helical | ||||
Sequence: VLALAWAVTVGGVLQLVYQLPH | ||||||
Topological domain | 208-223 | Cytoplasmic | ||||
Sequence: LKKIGMLVLPRINFHD | ||||||
Transmembrane | 224-253 | Helical | ||||
Sequence: AGAMRVVKQMGPAILGVSVSQISLIINTIF | ||||||
Topological domain | 254-263 | Periplasmic | ||||
Sequence: ASFLASGSVS | ||||||
Transmembrane | 264-292 | Helical | ||||
Sequence: WMYYADRLMEFPSGVLGVALGTILLPSLS | ||||||
Topological domain | 293-302 | Cytoplasmic | ||||
Sequence: KSFASGNHDE | ||||||
Transmembrane | 303-337 | Helical | ||||
Sequence: YNRLMDWGLRLCFLLALPSAVALGILSGPLTVSLF | ||||||
Topological domain | 338-346 | Periplasmic | ||||
Sequence: QYGKFTAFD | ||||||
Transmembrane | 347-374 | Helical | ||||
Sequence: ALMTQRALIAYSVGLIGLIVVKVLAPGF | ||||||
Topological domain | 375-379 | Cytoplasmic | ||||
Sequence: YSRQD | ||||||
Transmembrane | 380-402 | Helical | ||||
Sequence: IKTPVKIAIVTLILTQLMNLAFI | ||||||
Topological domain | 403-409 | Periplasmic | ||||
Sequence: GPLKHAG | ||||||
Transmembrane | 410-429 | Helical | ||||
Sequence: LSLSIGLAACLNASLLYWQL | ||||||
Topological domain | 430-443 | Cytoplasmic | ||||
Sequence: RKQKIFTPQPGWMA | ||||||
Transmembrane | 444-463 | Helical | ||||
Sequence: FLLRLVVAVLVMSGVLLGML | ||||||
Topological domain | 464-477 | Periplasmic | ||||
Sequence: HIMPEWSLGTMPWR | ||||||
Transmembrane | 478-499 | Helical | ||||
Sequence: LLRLMAVVLAGIAAYFAALAVL | ||||||
Topological domain | 500-511 | Cytoplasmic | ||||
Sequence: GFKVKEFARRTV |
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 18 | Lack of activity. | ||||
Sequence: R → A or C | ||||||
Mutagenesis | 24 | Lack of activity. | ||||
Sequence: R → A or C | ||||||
Mutagenesis | 39 | Lack of activity. | ||||
Sequence: D → A or C | ||||||
Mutagenesis | 52 | Decrease in activity. | ||||
Sequence: R → A | ||||||
Mutagenesis | 52 | Lack of activity. | ||||
Sequence: R → C | ||||||
Mutagenesis | 270 | Lack of activity. | ||||
Sequence: R → A or C |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000182006 | 1-511 | Lipid II flippase MurJ | |||
Sequence: MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVAFKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAGMLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIPAFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGVLQLVYQLPHLKKIGMLVLPRINFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPSGVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTVSLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLILTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVVAVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFARRTV |
Proteomic databases
Structure
Sequence
- Sequence statusComplete
- Length511
- Mass (Da)55,267
- Last updated2005-12-20 v1
- ChecksumCB20FE3CFC9419C2
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U00096 EMBL· GenBank· DDBJ | AAC74153.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP009048 EMBL· GenBank· DDBJ | BAA35877.1 EMBL· GenBank· DDBJ | Genomic DNA |