P0AE77 · COBU_ECO57

  • Protein
    Bifunctional adenosylcobalamin biosynthesis protein CobU
  • Gene
    cobU
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Catalyzes ATP-dependent phosphorylation of adenosylcobinamide and addition of GMP to adenosylcobinamide phosphate.

Catalytic activity

Pathway

Cofactor biosynthesis; adenosylcobalamin biosynthesis; adenosylcobalamin from cob(II)yrinate a,c-diamide: step 5/7.
Cofactor biosynthesis; adenosylcobalamin biosynthesis; adenosylcobalamin from cob(II)yrinate a,c-diamide: step 6/7.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site7-14GTP (UniProtKB | ChEBI)
Binding site31-33GTP (UniProtKB | ChEBI)
Active site47GMP-histidine intermediate
Binding site48-51GTP (UniProtKB | ChEBI)
Binding site59GTP (UniProtKB | ChEBI)
Binding site81GTP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Functionadenosylcobinamide kinase (ATP-specific) activity
Molecular Functionadenosylcobinamide kinase (GTP-specific) activity
Molecular FunctionATP binding
Molecular Functioncobinamide phosphate guanylyltransferase activity
Molecular FunctionGTP binding
Biological Processcobalamin biosynthetic process
Biological Processporphyrin-containing compound biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Bifunctional adenosylcobalamin biosynthesis protein CobU
  • Alternative names
    • Adenosylcobinamide kinase (EC:2.7.1.156
      ) . EC:2.7.1.156 (UniProtKB | ENZYME | Rhea)
    • Adenosylcobinamide-phosphate guanylyltransferase (EC:2.7.7.62
      ) . EC:2.7.7.62 (UniProtKB | ENZYME | Rhea)

Gene names

    • Name
      cobU
    • Ordered locus names
      Z3153, ECs2788

Organism names

  • Taxonomic identifier
  • Strains
    • O157:H7 / EDL933 / ATCC 700927 / EHEC
    • O157:H7 / Sakai / RIMD 0509952 / EHEC
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia

Accessions

  • Primary accession
    P0AE77
  • Secondary accessions
    • P46886

Proteomes

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000899971-181Bifunctional adenosylcobalamin biosynthesis protein CobU

Expression

Induction

By cobinamide.

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the CobU/CobP family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    181
  • Mass (Da)
    20,164
  • Last updated
    2005-12-06 v1
  • Checksum
    072EB233416EBCB1
MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARIEHHRQGRPEHWRTVERWQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKIK

Sequence caution

The sequence BAB36211.2 differs from that shown. Reason: Erroneous initiation Truncated N-terminus.

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AE005174
EMBL· GenBank· DDBJ
AAG57054.1
EMBL· GenBank· DDBJ
Genomic DNA
BA000007
EMBL· GenBank· DDBJ
BAB36211.2
EMBL· GenBank· DDBJ
Genomic DNA Different initiation

Genome annotation databases

Similar Proteins

Disclaimer

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