P0AE77 · COBU_ECO57
- ProteinBifunctional adenosylcobalamin biosynthesis protein CobU
- GenecobU
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids181 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Catalyzes ATP-dependent phosphorylation of adenosylcobinamide and addition of GMP to adenosylcobinamide phosphate.
Catalytic activity
- adenosylcob(III)inamide + GTP = adenosylcob(III)inamide phosphate + GDP + H+
Pathway
Cofactor biosynthesis; adenosylcobalamin biosynthesis; adenosylcobalamin from cob(II)yrinate a,c-diamide: step 5/7.
Cofactor biosynthesis; adenosylcobalamin biosynthesis; adenosylcobalamin from cob(II)yrinate a,c-diamide: step 6/7.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 7-14 | GTP (UniProtKB | ChEBI) | ||||
Sequence: GGARSGKS | ||||||
Binding site | 31-33 | GTP (UniProtKB | ChEBI) | ||||
Sequence: ATS | ||||||
Active site | 47 | GMP-histidine intermediate | ||||
Sequence: H | ||||||
Binding site | 48-51 | GTP (UniProtKB | ChEBI) | ||||
Sequence: RQGR | ||||||
Binding site | 59 | GTP (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 81 | GTP (UniProtKB | ChEBI) | ||||
Sequence: E |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | adenosylcobinamide kinase (ATP-specific) activity | |
Molecular Function | adenosylcobinamide kinase (GTP-specific) activity | |
Molecular Function | ATP binding | |
Molecular Function | cobinamide phosphate guanylyltransferase activity | |
Molecular Function | GTP binding | |
Biological Process | cobalamin biosynthetic process | |
Biological Process | porphyrin-containing compound biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameBifunctional adenosylcobalamin biosynthesis protein CobU
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionP0AE77
- Secondary accessions
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000089997 | 1-181 | Bifunctional adenosylcobalamin biosynthesis protein CobU | |||
Sequence: MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARIEHHRQGRPEHWRTVERWQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKIK |
Expression
Induction
By cobinamide.
Interaction
Structure
Sequence
- Sequence statusComplete
- Length181
- Mass (Da)20,164
- Last updated2005-12-06 v1
- Checksum072EB233416EBCB1
Sequence caution
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE005174 EMBL· GenBank· DDBJ | AAG57054.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BA000007 EMBL· GenBank· DDBJ | BAB36211.2 EMBL· GenBank· DDBJ | Genomic DNA | Different initiation |