P0ADF6 · EDD_ECOLI
- ProteinPhosphogluconate dehydratase
- Geneedd
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids603 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Catalyzes the dehydration of 6-phospho-D-gluconate to 2-dehydro-3-deoxy-6-phospho-D-gluconate.
Catalytic activity
- 6-phospho-D-gluconate = 2-dehydro-3-deoxy-6-phospho-D-gluconate + H2O
Cofactor
Note: Binds 1 [4Fe-4S] cluster.
Activity regulation
Sensitive to oxidants such as superoxide or hydrogen peroxide.
Pathway
Carbohydrate metabolism; Entner-Doudoroff pathway.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | metal ion binding | |
Molecular Function | phosphogluconate dehydratase activity | |
Biological Process | D-gluconate catabolic process | |
Biological Process | Entner-Doudoroff pathway through 6-phosphogluconate |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePhosphogluconate dehydratase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionP0ADF6
- Secondary accessions
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Phenotypes & Variants
Disruption phenotype
The mutant grows at normal rates on glucose and fructose, whereas on gluconate it grows about one-third as fast as the wild-type.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000103553 | 1-603 | Phosphogluconate dehydratase | |||
Sequence: MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGNLAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVGQVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPGLTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAPGTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWMPIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMARLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGELDWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVVFESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVTDGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAAREPHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF |
Proteomic databases
Expression
Induction
By growth on gluconate.
Structure
Sequence
- Sequence statusComplete
- Length603
- Mass (Da)64,639
- Last updated2005-12-06 v1
- ChecksumD046405193BFC185
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 252-254 | in Ref. 1; AAA23722 | ||||
Sequence: PLR → AC |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
M87458 EMBL· GenBank· DDBJ | AAA23722.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
X63694 EMBL· GenBank· DDBJ | CAA45221.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U00096 EMBL· GenBank· DDBJ | AAC74921.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP009048 EMBL· GenBank· DDBJ | BAA15659.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
L20897 EMBL· GenBank· DDBJ | AAA23863.1 EMBL· GenBank· DDBJ | Genomic DNA |