P0ADF6 · EDD_ECOLI

Function

function

Catalyzes the dehydration of 6-phospho-D-gluconate to 2-dehydro-3-deoxy-6-phospho-D-gluconate.

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster.

Activity regulation

Sensitive to oxidants such as superoxide or hydrogen peroxide.

Pathway

Carbohydrate metabolism; Entner-Doudoroff pathway.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site154[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site221[4Fe-4S] cluster (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functionmetal ion binding
Molecular Functionphosphogluconate dehydratase activity
Biological ProcessD-gluconate catabolic process
Biological ProcessEntner-Doudoroff pathway through 6-phosphogluconate

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Phosphogluconate dehydratase
  • EC number
  • Alternative names
    • 6-phosphogluconate dehydratase
    • Entner-Doudoroff dehydrase

Gene names

    • Name
      edd
    • Ordered locus names
      b1851, JW1840

Organism names

  • Taxonomic identifier
  • Strains
    • K12
    • K12 / W3110 / ATCC 27325 / DSM 5911
    • K12 / MG1655 / ATCC 47076
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia

Accessions

  • Primary accession
    P0ADF6
  • Secondary accessions
    • P25530

Proteomes

Subcellular Location

Phenotypes & Variants

Disruption phenotype

The mutant grows at normal rates on glucose and fructose, whereas on gluconate it grows about one-third as fast as the wild-type.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001035531-603Phosphogluconate dehydratase

Proteomic databases

Expression

Induction

By growth on gluconate.

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the IlvD/Edd family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    603
  • Mass (Da)
    64,639
  • Last updated
    2005-12-06 v1
  • Checksum
    D046405193BFC185
MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGNLAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVGQVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPGLTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAPGTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWMPIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMARLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGELDWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVVFESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVTDGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAAREPHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict252-254in Ref. 1; AAA23722

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
M87458
EMBL· GenBank· DDBJ
AAA23722.1
EMBL· GenBank· DDBJ
Genomic DNA
X63694
EMBL· GenBank· DDBJ
CAA45221.1
EMBL· GenBank· DDBJ
Genomic DNA
U00096
EMBL· GenBank· DDBJ
AAC74921.1
EMBL· GenBank· DDBJ
Genomic DNA
AP009048
EMBL· GenBank· DDBJ
BAA15659.1
EMBL· GenBank· DDBJ
Genomic DNA
L20897
EMBL· GenBank· DDBJ
AAA23863.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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