P0ADA3 · NLPD_ECOLI

Function

function

Activator of the cell wall hydrolase AmiC. Required for septal murein cleavage and daughter cell separation during cell division.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcell division site
Cellular Componentcell outer membrane
Cellular Componentplasma membrane
Molecular Functionmetalloendopeptidase activity
Biological Processcell division
Biological Processresponse to xenobiotic stimulus

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Murein hydrolase activator NlpD

Gene names

    • Name
      nlpD
    • Ordered locus names
      b2742, JW2712

Organism names

  • Taxonomic identifier
  • Strains
    • MP180
    • K12 / MG1655 / ATCC 47076
    • K12 / W3110 / ATCC 27325 / DSM 5911
    • K12 / DH1 / ATCC 33849 / DSM 4235 / NCIB 12045
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia

Accessions

  • Primary accession
    P0ADA3
  • Secondary accessions
    • P33648
    • Q2MA87

Proteomes

Subcellular Location

Cell inner membrane
; Lipid-anchor
Note: Localizes at the septal ring.

Keywords

Phenotypes & Variants

Disruption phenotype

Cells are shorter in a single mutant, double envC-nlpD disruptions have defects in septation and cell separation and form long filaments (8-fold longer). Cell length increase is more exacerbated with a triple mepM (yebA) or ygeR disruption (15-fold longer) and further yet by the quadruple disruption mutant (envC-nlpD-mepM(yebA)-ygeR, over 21-fold longer). Quadruple mutants are less sensitive to ampicillin lysis.

PTM/Processing

Features

Showing features for signal, lipidation, chain.

TypeIDPosition(s)Description
Signal1-25
Lipidation26N-palmitoyl cysteine
Lipidation26S-diacylglycerol cysteine
ChainPRO_000001802926-379Murein hydrolase activator NlpD

Keywords

Proteomic databases

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region, repeat, domain.

TypeIDPosition(s)Description
Region30-67Disordered
Repeat66-731-1
Region66-974 X 8 AA tandem repeats of Q-Q-P-Q-I-Q-P-V
Repeat74-811-2; approximate
Repeat82-891-3
Repeat90-971-4; approximate
Domain121-165LysM
Repeat205-2112-1
Region205-2524 X 7 AA approximate repeats
Region210-231Disordered
Repeat227-2332-2
Repeat239-2452-3
Region240-259Disordered
Repeat246-2522-4

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    379
  • Mass (Da)
    40,149
  • Last updated
    2005-12-06 v1
  • Checksum
    A8E6A2B8456105FE
MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict139in Ref. 4; BAA04487

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
L07869
EMBL· GenBank· DDBJ
AAA17875.1
EMBL· GenBank· DDBJ
Unassigned DNA
U29579
EMBL· GenBank· DDBJ
AAA69252.1
EMBL· GenBank· DDBJ
Genomic DNA
U00096
EMBL· GenBank· DDBJ
AAC75784.1
EMBL· GenBank· DDBJ
Genomic DNA
AP009048
EMBL· GenBank· DDBJ
BAE76819.1
EMBL· GenBank· DDBJ
Genomic DNA
D17549
EMBL· GenBank· DDBJ
BAA04487.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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