P0AC19 · FOLX_ECOLI
- ProteinDihydroneopterin triphosphate 2'-epimerase
- GenefolX
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids120 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the epimerization of carbon 2' of the side chain of 7,8-dihydroneopterin triphosphate (H2NTP) to form 7,8-dihydromonapterin triphosphate (H2MTP) (PubMed:9182560, PubMed:9651328).
Is required for tetrahydromonapterin biosynthesis, a major pterin in E.coli (PubMed:19897652).
Is required for tetrahydromonapterin biosynthesis, a major pterin in E.coli (PubMed:19897652).
Catalytic activity
- 7,8-dihydroneopterin 3'-triphosphate = 7,8-dihydromonapterin 3'-triphosphate
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
13 μM | 7,8-dihydroneopterin triphosphate | |||||
149 μM | 7,8-dihydroneopterin | |||||
66 μM | 7,8-dihydromonapterin |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
480 μmol/h/mg | for the epimerization of 7,8-dihydroneopterin triphosphate | ||||
0.95 μmol/h/mg | for the epimerization of 7,8-dihydroneopterin | ||||
0.67 μmol/h/mg | for the epimerization of 7,8-dihydromonapterin |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | cytosol | |
Molecular Function | dihydroneopterin aldolase activity | |
Molecular Function | dihydroneopterin triphosphate 2'-epimerase activity | |
Molecular Function | identical protein binding | |
Biological Process | folic acid-containing compound metabolic process | |
Biological Process | pteridine-containing compound biosynthetic process |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDihydroneopterin triphosphate 2'-epimerase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionP0AC19
- Secondary accessions
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Phenotypes & Variants
PTM/Processing
Features
Showing features for initiator methionine, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Initiator methionine | 1 | Removed | ||||
Sequence: M | ||||||
Chain | PRO_0000168295 | 2-120 | Dihydroneopterin triphosphate 2'-epimerase | |||
Sequence: AQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADKARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDKLHALRYADSVSMTLSWQR |
Proteomic databases
Structure
Sequence
- Sequence statusComplete
- Length120
- Mass (Da)14,082
- Last updated2007-01-23 v2
- Checksum5DDFF76540827ED6
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 27-30 | in Ref. 2; AAB47972 | ||||
Sequence: NNRQ → KQPS | ||||||
Sequence conflict | 48-49 | in Ref. 2; AAB47972 | ||||
Sequence: SE → RQ | ||||||
Sequence conflict | 55 | in Ref. 2; AAB47972 | ||||
Sequence: L → M | ||||||
Sequence conflict | 107 | in Ref. 2; AAB47972 | ||||
Sequence: R → A |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X96709 EMBL· GenBank· DDBJ | CAA65471.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U47639 EMBL· GenBank· DDBJ | AAB47972.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP009048 EMBL· GenBank· DDBJ | BAA16140.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U00096 EMBL· GenBank· DDBJ | AAC75363.1 EMBL· GenBank· DDBJ | Genomic DNA |