P0ABR9 · MHPB_ECOLI

  • Protein
    2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase
  • Gene
    mhpB
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Catalyzes the non-heme iron(II)-dependent oxidative cleavage of 2,3-dihydroxyphenylpropionic acid and 2,3-dihydroxicinnamic acid into 2-hydroxy-6-ketononadienedioate and 2-hydroxy-6-ketononatrienedioate, respectively.

Catalytic activity

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
26 μM2,3-dihydroxyphenylpropionic acid820
36 μM2,3-dihydroxycinnamic acid820
37 μMmethyl-2,3-dihydroxyphenylpropionate820
90 μM3-methylcatechol820
94 μM3-phenethylcatechol820
154 μM3-propylcatechol820
185 μM3-ethylcatechol820
300 μM2,3-dihydroxyphenoxyacetic acid820
700 μMcatechol820

Pathway

Aromatic compound metabolism; 3-phenylpropanoate degradation.

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site115Proton donor
Active site179Proton acceptor

GO annotations

AspectTerm
Molecular Function3-carboxyethylcatechol 2,3-dioxygenase activity
Molecular Functionferrous iron binding
Biological Process3-(3-hydroxy)phenylpropionate catabolic process
Biological Process3-phenylpropionate catabolic process
Biological Processphenylpropanoid catabolic process

Keywords

Enzyme and pathway databases

Chemistry

Names & Taxonomy

Protein names

  • Recommended name
    2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase
  • EC number
  • Alternative names
    • 3-carboxyethylcatechol 2,3-dioxygenase

Gene names

    • Name
      mhpB
    • Ordered locus names
      b0348, JW0339

Organism names

  • Taxonomic identifier
  • Strains
    • K12 / W3110 / ATCC 27325 / DSM 5911
    • K12 / CS520
    • K12 / MG1655 / ATCC 47076
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia

Accessions

  • Primary accession
    P0ABR9
  • Secondary accessions
    • P54711
    • P77048
    • P77461
    • Q2MC76

Proteomes

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis114Complete loss of extradiol cleavage activity.
Mutagenesis114Low level of catalytic activity, 600-fold lower than the wild-type enzyme. More than 8000-fold decrease in affinity.
Mutagenesis115Complete loss of extradiol cleavage activity.
Mutagenesis115Complete loss of activity.
Mutagenesis115Complete loss of activity.
Mutagenesis179Complete loss of activity.
Mutagenesis179Complete loss of activity.
Mutagenesis179Complete loss of activity.
Mutagenesis181More than 2-fold decrease in catalytic activity and 100-fold decrease in affinity.
Mutagenesis181More than 60-fold decrease in catalytic activity and affinity.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000851031-3142,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase

Proteomic databases

Interaction

Subunit

Homotetramer.

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    314
  • Mass (Da)
    34,196
  • Last updated
    2005-11-08 v1
  • Checksum
    E1D5A8574E5DFE05
MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPELVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSGIDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGRFTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVISAAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWVAAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict138-140in Ref. 1; BAA13053
Sequence conflict152in Ref. 1; BAA13053
Sequence conflict157in Ref. 1; BAA13053

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
D86239
EMBL· GenBank· DDBJ
BAA13053.1
EMBL· GenBank· DDBJ
Genomic DNA
Y09555
EMBL· GenBank· DDBJ
CAA70748.1
EMBL· GenBank· DDBJ
Genomic DNA
U73857
EMBL· GenBank· DDBJ
AAB18072.1
EMBL· GenBank· DDBJ
Genomic DNA
U00096
EMBL· GenBank· DDBJ
AAC73451.1
EMBL· GenBank· DDBJ
Genomic DNA
AP009048
EMBL· GenBank· DDBJ
BAE76130.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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