P0ABN7 · DCUA_ECO57
- ProteinC4-dicarboxylate transporter DcuA
- GenedcuA
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids433 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Responsible for the transport of C4-dicarboxylates during aerobic and anaerobic growth. Required for the uptake of L-aspartate as a nitrogen source during aerobic growth. The uptake of L-aspartate in aerobic conditions is coupled to the excretion of fumarate, resulting in the net uptake of nitrogen without carbon uptake. In addition, during anaerobic growth, catalyzes the uptake of fumarate, malate or aspartate coupled to the export of succinate. May play a a general role in anaerobic C4-dicarboxylate transport.
Catalytic activity
- fumarate(in) + L-aspartate(out) = fumarate(out) + L-aspartate(in)This reaction proceeds in the forward direction.fumarate (in)CHEBI:29806
+ L-aspartate (out)CHEBI:29991= fumarate (out)CHEBI:29806+ L-aspartate (in)CHEBI:29991 - fumarate(in) + succinate(out) = fumarate(out) + succinate(in)This reaction proceeds in the backward direction.
- (S)-malate(in) + succinate(out) = (S)-malate(out) + succinate(in)This reaction proceeds in the backward direction.
- L-aspartate(in) + succinate(out) = L-aspartate(out) + succinate(in)This reaction proceeds in the backward direction.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | plasma membrane | |
Molecular Function | antiporter activity | |
Molecular Function | C4-dicarboxylate transmembrane transporter activity |
Keywords
- Biological process
Names & Taxonomy
Protein names
- Recommended nameC4-dicarboxylate transporter DcuA
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionP0ABN7
- Secondary accessions
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Cell inner membrane ; Multi-pass membrane protein
Features
Showing features for transmembrane, topological domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Transmembrane | 1-18 | Helical | |||
Topological domain | 19 | Cytoplasmic | |||
Transmembrane | 20-37 | Helical | |||
Topological domain | 38-53 | Periplasmic | |||
Transmembrane | 54-71 | Helical | |||
Topological domain | 72-85 | Cytoplasmic | |||
Transmembrane | 86-103 | Helical | |||
Topological domain | 104-132 | Periplasmic | |||
Transmembrane | 133-147 | Helical | |||
Topological domain | 148-228 | Cytoplasmic | |||
Transmembrane | 229-246 | Helical | |||
Topological domain | 247-264 | Periplasmic | |||
Transmembrane | 265-282 | Helical | |||
Topological domain | 283-292 | Cytoplasmic | |||
Transmembrane | 293-310 | Helical | |||
Topological domain | 311-332 | Periplasmic | |||
Transmembrane | 333-350 | Helical | |||
Topological domain | 351-355 | Cytoplasmic | |||
Transmembrane | 356-373 | Helical | |||
Topological domain | 374-433 | Periplasmic | |||
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000170348 | 1-433 | C4-dicarboxylate transporter DcuA | ||
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length433
- Mass (Da)45,751
- Last updated2005-11-08 v1
- Checksum049C8D186C83C9AD
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE005174 EMBL· GenBank· DDBJ | AAG59337.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BA000007 EMBL· GenBank· DDBJ | BAB38542.1 EMBL· GenBank· DDBJ | Genomic DNA |