P0ABN7 · DCUA_ECO57

  • Protein
    C4-dicarboxylate transporter DcuA
  • Gene
    dcuA
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Responsible for the transport of C4-dicarboxylates during aerobic and anaerobic growth. Required for the uptake of L-aspartate as a nitrogen source during aerobic growth. The uptake of L-aspartate in aerobic conditions is coupled to the excretion of fumarate, resulting in the net uptake of nitrogen without carbon uptake. In addition, during anaerobic growth, catalyzes the uptake of fumarate, malate or aspartate coupled to the export of succinate. May play a a general role in anaerobic C4-dicarboxylate transport.

Catalytic activity

  • fumarate(in) + L-aspartate(out) = fumarate(out) + L-aspartate(in)
    This reaction proceeds in the forward direction.
  • fumarate(in) + succinate(out) = fumarate(out) + succinate(in)
    This reaction proceeds in the backward direction.
  • (S)-malate(in) + succinate(out) = (S)-malate(out) + succinate(in)
    This reaction proceeds in the backward direction.
  • L-aspartate(in) + succinate(out) = L-aspartate(out) + succinate(in)
    This reaction proceeds in the backward direction.

GO annotations

AspectTerm
Cellular Componentplasma membrane
Molecular Functionantiporter activity
Molecular FunctionC4-dicarboxylate transmembrane transporter activity

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    C4-dicarboxylate transporter DcuA

Gene names

    • Name
      dcuA
    • Ordered locus names
      Z5743, ECs5119

Organism names

  • Taxonomic identifier
  • Strains
    • O157:H7 / EDL933 / ATCC 700927 / EHEC
    • O157:H7 / Sakai / RIMD 0509952 / EHEC
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia

Accessions

  • Primary accession
    P0ABN7
  • Secondary accessions
    • P04539

Proteomes

Subcellular Location

Cell inner membrane
; Multi-pass membrane protein

Features

Showing features for transmembrane, topological domain.

Type
IDPosition(s)Description
Transmembrane1-18Helical
Topological domain19Cytoplasmic
Transmembrane20-37Helical
Topological domain38-53Periplasmic
Transmembrane54-71Helical
Topological domain72-85Cytoplasmic
Transmembrane86-103Helical
Topological domain104-132Periplasmic
Transmembrane133-147Helical
Topological domain148-228Cytoplasmic
Transmembrane229-246Helical
Topological domain247-264Periplasmic
Transmembrane265-282Helical
Topological domain283-292Cytoplasmic
Transmembrane293-310Helical
Topological domain311-332Periplasmic
Transmembrane333-350Helical
Topological domain351-355Cytoplasmic
Transmembrane356-373Helical
Topological domain374-433Periplasmic

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001703481-433C4-dicarboxylate transporter DcuA

Interaction

Protein-protein interaction databases

Family & Domains

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    433
  • Mass (Da)
    45,751
  • Last updated
    2005-11-08 v1
  • Checksum
    049C8D186C83C9AD
MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGNIPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFAGTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGHGISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIEIKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVICKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLAVIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQMDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AE005174
EMBL· GenBank· DDBJ
AAG59337.1
EMBL· GenBank· DDBJ
Genomic DNA
BA000007
EMBL· GenBank· DDBJ
BAB38542.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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