P0ABF1 · PCNB_ECOLI
- ProteinPoly(A) polymerase I
- GenepcnB
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids465 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Adds poly(A) tail to the 3' end of many RNAs, which usually targets these RNAs for decay. Plays a significant role in the global control of gene expression, through influencing the rate of transcript degradation, and in the general RNA quality control. Rho-independent transcription terminators may serve as polyadenylation signals. Seems to be involved in plasmid copy number control.
Miscellaneous
Uses the non-canonical initiation codon AUU, which limits pcnB expression.
Catalytic activity
- ATP + RNA(n) = diphosphate + RNA(n)-3'-adenine ribonucleotide
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 80 | |||||
Sequence: D | ||||||
Active site | 82 | |||||
Sequence: D | ||||||
Active site | 162 | |||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | cytosol | |
Cellular Component | plasma membrane | |
Molecular Function | ATP binding | |
Molecular Function | poly(A) RNA polymerase activity | |
Molecular Function | RNA binding | |
Biological Process | mRNA processing | |
Biological Process | plasmid maintenance | |
Biological Process | polyadenylation-dependent mRNA catabolic process | |
Biological Process | polyadenylation-dependent RNA catabolic process | |
Biological Process | RNA modification |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePoly(A) polymerase I
- EC number
- Short namesPAP I
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionP0ABF1
- Secondary accessions
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for propeptide, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Propeptide | PRO_0000411163 | 1-10 | ||||
Sequence: MFTRVANFCR | ||||||
Chain | PRO_0000139090 | 11-465 | Poly(A) polymerase I | |||
Sequence: KVLSREESEAEQAVARPQVTVIPREQHAISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRRTRRPRKRAPRREGTA |
Proteomic databases
Expression
Induction
Transcription regulation of pcnB is a complex process that involves several factors such as Sigma-70 (rpoD), Sigma-S (rpoS), ppGpp and DksA.
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 429-465 | Disordered | ||||
Sequence: SAPPDQKGMLNELDEEPSPRRRTRRPRKRAPRREGTA |
Sequence similarities
Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length465
- Mass (Da)53,871
- Last updated2006-09-19 v2
- Checksum0FAE30F2F95FB76E
Sequence caution
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 93 | in Ref. 1; AAA24303 | ||||
Sequence: R → A | ||||||
Sequence conflict | 315 | in Ref. 1; AAA24303 | ||||
Sequence: A → T | ||||||
Sequence conflict | 332 | in Ref. 1; AAA24303 | ||||
Sequence: K → N | ||||||
Sequence conflict | 437-438 | in Ref. 1; AAA24303 | ||||
Sequence: ML → IV | ||||||
Sequence conflict | 449-465 | in Ref. 1; AAA24303 | ||||
Sequence: RRTRRPRKRAPRREGTA → QSYSSSTQTRTTS |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
M20574 EMBL· GenBank· DDBJ | AAA24303.1 EMBL· GenBank· DDBJ | Genomic DNA | Different initiation | |
U00096 EMBL· GenBank· DDBJ | AAC73254.2 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP009048 EMBL· GenBank· DDBJ | BAB96720.2 EMBL· GenBank· DDBJ | Genomic DNA | ||
S48039 EMBL· GenBank· DDBJ | AAB24139.1 EMBL· GenBank· DDBJ | Genomic DNA | Different initiation |