P0AAJ3 · FDNH_ECOLI
- ProteinFormate dehydrogenase, nitrate-inducible, iron-sulfur subunit
- GenefdnH
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids294 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Formate dehydrogenase allows E.coli to use formate as major electron donor during anaerobic respiration, when nitrate is used as electron acceptor. The beta subunit FdnH is an electron transfer unit containing 4 iron-sulfur clusters; it serves as a conduit for electrons that are transferred from the formate oxidation site in the alpha subunit (FdnG) to the menaquinone associated with the gamma subunit (FdnI) of formate dehydrogenase-N. Formate dehydrogenase-N is part of a system that generates proton motive force, together with the dissimilatory nitrate reductase (Nar).
Miscellaneous
The 4Fe-4S clusters from PubMed:11884747 are renumbered in standard order.
Cofactor
Note: Binds 4 [4Fe-4S] clusters per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 39 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 42 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 45 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 49 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 100 | [4Fe-4S] cluster 3 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 103 | [4Fe-4S] cluster 3 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 108 | [4Fe-4S] cluster 3 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 112 | [4Fe-4S] cluster 4 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 133 | [4Fe-4S] cluster 4 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 136 | [4Fe-4S] cluster 4 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 139 | [4Fe-4S] cluster 4 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 143 | [4Fe-4S] cluster 3 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 160 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 163 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 175 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 179 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | formate dehydrogenase complex | |
Cellular Component | membrane | |
Cellular Component | plasma membrane | |
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | electron transfer activity | |
Molecular Function | formate dehydrogenase (quinone) activity | |
Molecular Function | metal ion binding | |
Biological Process | anaerobic electron transport chain | |
Biological Process | anaerobic respiration | |
Biological Process | formate oxidation | |
Biological Process | heme oxidation |
Keywords
- Biological process
- Ligand
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameFormate dehydrogenase, nitrate-inducible, iron-sulfur subunit
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionP0AAJ3
- Secondary accessions
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Cell inner membrane ; Single-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-256 | Periplasmic | ||||
Sequence: MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKACQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMHCEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKCTLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEGVGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGA | ||||||
Transmembrane | 257-279 | Helical | ||||
Sequence: LKPLAAAGFIATFAGLIFHYIGI | ||||||
Topological domain | 280-294 | Cytoplasmic | ||||
Sequence: GPNKEVDDDEEDHHE |
Keywords
- Cellular component
Phenotypes & Variants
Chemistry
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000159247 | 1-294 | Formate dehydrogenase, nitrate-inducible, iron-sulfur subunit | |||
Sequence: MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKACQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMHCEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKCTLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEGVGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHYIGIGPNKEVDDDEEDHHE |
Proteomic databases
Expression
Induction
By nitrate under anaerobic conditions.
Interaction
Subunit
Trimer of heterotrimers, consisting of subunits alpha, beta and gamma.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 30-58 | 4Fe-4S ferredoxin-type 1 | ||||
Sequence: VAKLIDVSTCIGCKACQVACSEWNDIRDE | ||||||
Domain | 91-123 | 4Fe-4S ferredoxin-type 2 | ||||
Sequence: LEWLIRKDGCMHCEDPGCLKACPSAGAIIQYAN | ||||||
Domain | 124-153 | 4Fe-4S ferredoxin-type 3 | ||||
Sequence: GIVDFQSENCIGCGYCIAGCPFNIPRLNKE | ||||||
Domain | 158-189 | 4Fe-4S ferredoxin-type 4 | ||||
Sequence: YKCTLCVDRVSVGQEPACVKTCPTGAIHFGTK |
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length294
- Mass (Da)32,239
- Last updated2005-10-11 v1
- Checksum44F361F4AC0AEF36
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 101-102 | in Ref. 1 | ||||
Sequence: MH → ID |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
M75029 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
U00096 EMBL· GenBank· DDBJ | AAD13439.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP009048 EMBL· GenBank· DDBJ | BAA15124.1 EMBL· GenBank· DDBJ | Genomic DNA |