P0A959 · ALAA_ECOLI
- ProteinGlutamate-pyruvate aminotransferase AlaA
- GenealaA
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids405 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Involved in the biosynthesis of alanine. Catalyzes the transamination of pyruvate by glutamate, leading to the formation of L-alanine and 2-oxoglutarate. Is also able to catalyze the reverse reaction.
Catalytic activity
- L-alanine + 2-oxoglutarate = pyruvate + L-glutamateThis reaction proceeds in the backward direction.
Cofactor
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.55 mM | pyruvate | 8.5 | 37 | |||
4.9 mM | alanine | 8.5 | 37 |
Pathway
Amino-acid biosynthesis; L-alanine biosynthesis.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | L-alanine:2-oxoglutarate aminotransferase activity | |
Molecular Function | protein homodimerization activity | |
Molecular Function | pyridoxal phosphate binding | |
Molecular Function | transaminase activity | |
Biological Process | alanine biosynthetic process | |
Biological Process | D-alanine biosynthetic process | |
Biological Process | DNA damage response | |
Biological Process | L-alanine biosynthetic process from pyruvate | |
Biological Process | response to antibiotic |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameGlutamate-pyruvate aminotransferase AlaA
- EC number
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionP0A959
- Secondary accessions
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000123866 | 1-405 | Glutamate-pyruvate aminotransferase AlaA | |||
Sequence: MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPAPFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGNGVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDDIRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDAEHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRLCANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYMFPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSLSKFARFLSGYHQL | ||||||
Modified residue | 240 | N6-(pyridoxal phosphate)lysine | ||||
Sequence: K |
Proteomic databases
Expression
Induction
Modestly repressed by alanine and leucine via Lrp.
Structure
Sequence
- Sequence statusComplete
- Length405
- Mass (Da)45,517
- Last updated2005-07-19 v1
- Checksum6A5E78876CC3C388
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U00096 EMBL· GenBank· DDBJ | AAC75350.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP009048 EMBL· GenBank· DDBJ | BAA16127.1 EMBL· GenBank· DDBJ | Genomic DNA |