P0A7E1 · PYRD_ECOLI

Function

function

Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor.

Catalytic activity

Cofactor

FMN (UniProtKB | Rhea| CHEBI:58210 )

Note: Binds 1 FMN per subunit.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
28.8 μMdihydroorotate
Vmax pH TEMPERATURE[C] NOTES EVIDENCE
180 μmol/min/mg

Pathway

Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site62-66FMN (UniProtKB | ChEBI)
Binding site66substrate
Binding site86FMN (UniProtKB | ChEBI)
Binding site111-115substrate
Binding site139FMN (UniProtKB | ChEBI)
Binding site172FMN (UniProtKB | ChEBI)
Binding site172substrate
Active site175Nucleophile
Binding site177substrate
Binding site217FMN (UniProtKB | ChEBI)
Binding site245FMN (UniProtKB | ChEBI)
Binding site246-247substrate
Binding site268FMN (UniProtKB | ChEBI)
Binding site297FMN (UniProtKB | ChEBI)
Binding site318-319FMN (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Cellular Componentmembrane
Cellular Componentplasma membrane
Molecular Functiondihydroorotate dehydrogenase (quinone) activity
Molecular Functiondihydroorotate dehydrogenase activity
Molecular FunctionFMN binding
Biological Process'de novo' pyrimidine nucleobase biosynthetic process
Biological Process'de novo' UMP biosynthetic process
Biological Processpyrimidine ribonucleotide biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Dihydroorotate dehydrogenase (quinone)
  • EC number
  • Alternative names
    • DHOdehase (DHOD; DHODase)
    • Dihydroorotate oxidase

Gene names

    • Name
      pyrD
    • Ordered locus names
      b0945, JW0928

Organism names

  • Taxonomic identifier
  • Strains
    • K12 / W3110 / ATCC 27325 / DSM 5911
    • K12 / MG1655 / ATCC 47076
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia

Accessions

  • Primary accession
    P0A7E1
  • Secondary accessions
    • P05021

Proteomes

Subcellular Location

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis175Almost no activity.
Mutagenesis175500-fold reduction in activity.

Chemistry

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001484371-336Dihydroorotate dehydrogenase (quinone)

Proteomic databases

Interaction

Subunit

Monomer.

Protein-protein interaction databases

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    336
  • Mass (Da)
    36,775
  • Last updated
    1987-08-13 v1
  • Checksum
    973227EAE6B83622
MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPAKPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFRLVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICMEKIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKIAPDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKSTEIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVTHI

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X02826
EMBL· GenBank· DDBJ
CAA26594.1
EMBL· GenBank· DDBJ
Genomic DNA
U00096
EMBL· GenBank· DDBJ
AAC74031.1
EMBL· GenBank· DDBJ
Genomic DNA
AP009048
EMBL· GenBank· DDBJ
BAA35700.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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