P0A7E1 · PYRD_ECOLI
- ProteinDihydroorotate dehydrogenase (quinone)
- GenepyrD
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids336 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor.
Catalytic activity
- (S)-dihydroorotate + a quinone = a quinol + orotate
Cofactor
Note: Binds 1 FMN per subunit.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
28.8 μM | dihydroorotate |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
180 μmol/min/mg |
Pathway
Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 62-66 | FMN (UniProtKB | ChEBI) | ||||
Sequence: AGLDK | ||||||
Binding site | 66 | substrate | ||||
Sequence: K | ||||||
Binding site | 86 | FMN (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 111-115 | substrate | ||||
Sequence: NRMGF | ||||||
Binding site | 139 | FMN (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 172 | FMN (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 172 | substrate | ||||
Sequence: N | ||||||
Active site | 175 | Nucleophile | ||||
Sequence: S | ||||||
Binding site | 177 | substrate | ||||
Sequence: N | ||||||
Binding site | 217 | FMN (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 245 | FMN (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 246-247 | substrate | ||||
Sequence: NT | ||||||
Binding site | 268 | FMN (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 297 | FMN (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 318-319 | FMN (UniProtKB | ChEBI) | ||||
Sequence: YS |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | membrane | |
Cellular Component | plasma membrane | |
Molecular Function | dihydroorotate dehydrogenase (quinone) activity | |
Molecular Function | dihydroorotate dehydrogenase activity | |
Molecular Function | FMN binding | |
Biological Process | 'de novo' pyrimidine nucleobase biosynthetic process | |
Biological Process | 'de novo' UMP biosynthetic process | |
Biological Process | pyrimidine ribonucleotide biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDihydroorotate dehydrogenase (quinone)
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionP0A7E1
- Secondary accessions
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Peripheral membrane protein
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 175 | Almost no activity. | ||||
Sequence: S → A | ||||||
Mutagenesis | 175 | 500-fold reduction in activity. | ||||
Sequence: S → C |
Chemistry
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000148437 | 1-336 | Dihydroorotate dehydrogenase (quinone) | |||
Sequence: MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPAKPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFRLVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICMEKIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKIAPDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKSTEIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVTHI |
Proteomic databases
Structure
Sequence
- Sequence statusComplete
- Length336
- Mass (Da)36,775
- Last updated1987-08-13 v1
- Checksum973227EAE6B83622
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X02826 EMBL· GenBank· DDBJ | CAA26594.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U00096 EMBL· GenBank· DDBJ | AAC74031.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP009048 EMBL· GenBank· DDBJ | BAA35700.1 EMBL· GenBank· DDBJ | Genomic DNA |