P0A759 · NAGB_ECOLI

Function

function

Catalyzes the reversible isomerization-deamination of glucosamine 6-phosphate (GlcN6P) to form fructose 6-phosphate (Fru6P) and ammonium ion.

Miscellaneous

Disulfide bonds seem not to be essential for the stability of the oligomer under physiological conditions.

Catalytic activity

Activity regulation

Allosterically activated by N-acetylglucosamine 6-phosphate (GlcNAc6P). Competitively inhibited by 2-amino-2-deoxy-D-glucitol-6-phosphate (GlcN-ol-6P).

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
0.5 mMGlcN6P

Pathway

Amino-sugar metabolism; N-acetylneuraminate degradation; D-fructose 6-phosphate from N-acetylneuraminate: step 5/5.

Features

Showing features for active site, site.

TypeIDPosition(s)Description
Active site72Proton acceptor; for enolization step
Active site141For ring-opening step
Active site143Proton acceptor; for ring-opening step
Active site148For ring-opening step
Site151Part of the allosteric site
Site158Part of the allosteric site
Site160Part of the allosteric site
Site161Part of the allosteric site
Site254Part of the allosteric site

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentcytosol
Molecular Functionglucosamine-6-phosphate deaminase activity
Molecular Functionidentical protein binding
Biological Processcarbohydrate metabolic process
Biological Processglucosamine catabolic process
Biological ProcessN-acetylglucosamine catabolic process
Biological ProcessN-acetylneuraminate catabolic process
Biological ProcessUDP-N-acetylglucosamine biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Glucosamine-6-phosphate deaminase
  • EC number
  • Alternative names
    • GlcN6P deaminase (GNPDA)
    • Glucosamine-6-phosphate isomerase

Gene names

    • Name
      nagB
    • Synonyms
      glmD
    • Ordered locus names
      b0678, JW0664

Organism names

  • Taxonomic identifier
  • Strains
    • K12
    • K12 / W3110 / ATCC 27325 / DSM 5911
    • K12 / MG1655 / ATCC 47076
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia

Accessions

  • Primary accession
    P0A759
  • Secondary accessions
    • O68603
    • P09375

Proteomes

Subcellular Location

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis11850% of wild-type activity, but 2-fold decrease in substrate affinity.
Mutagenesis141Large decrease in activity.
Mutagenesis143Loss of activity for the deamination reaction but not for the reverse reaction; complete loss of the homotropic cooperativity.
Mutagenesis148Large decrease in activity.
Mutagenesis174Loss of activity in the absence of the allosteric activator.
Mutagenesis23950% of wild-type activity, but 2-fold decrease in substrate affinity; decrease in allosteric interaction energy.

Chemistry

PTM/Processing

Features

Showing features for chain, disulfide bond.

TypeIDPosition(s)Description
ChainPRO_00001601431-266Glucosamine-6-phosphate deaminase
Disulfide bond219Interchain

Keywords

Proteomic databases

Interaction

Subunit

Homohexamer; trimer of disulfide-linked dimers.

Protein-protein interaction databases

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    266
  • Mass (Da)
    29,774
  • Last updated
    2005-06-07 v1
  • Checksum
    D1443A40E74AC08E
MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGTPMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAENINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIKTLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEGCVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict70in Ref. 2; AAC09324
Sequence conflict91in Ref. 2; AAC09324

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
M19284
EMBL· GenBank· DDBJ
AAA24191.1
EMBL· GenBank· DDBJ
Genomic DNA
AF052007
EMBL· GenBank· DDBJ
AAC09324.1
EMBL· GenBank· DDBJ
Genomic DNA
U00096
EMBL· GenBank· DDBJ
AAC73772.1
EMBL· GenBank· DDBJ
Genomic DNA
AP009048
EMBL· GenBank· DDBJ
BAA35321.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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