P0A6V8 · GLK_ECOLI

Function

function

An ATP-dependent kinase that phosphorylates glucose to make glucose-6-phosphate, not active on fructose, galactose or mannose. Not highly important in wild-type E.coli as glucose is transported into the cell by the PTS system as glucose 6-phosphate.

Catalytic activity

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
0.78 mMglucose
3.76 mMATP

Features

Showing features for binding site.

132150100150200250300
TypeIDPosition(s)Description
Binding site8-13ATP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular FunctionATP binding
Molecular FunctionD-glucose binding
Molecular Functionglucokinase activity
Biological Processglycolytic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Glucokinase
  • EC number
  • Alternative names
    • Glucose kinase

Gene names

    • Name
      glk
    • Ordered locus names
      b2388, JW2385

Organism names

  • Taxonomic identifier
  • Strains
    • K12 / MC4100 / ATCC 35695 / DSM 6574
    • K12 / W3110 / ATCC 27325 / DSM 5911
    • K12 / MG1655 / ATCC 47076
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia

Accessions

  • Primary accession
    P0A6V8
  • Secondary accessions
    • P46880
    • P78276

Proteomes

Subcellular Location

Keywords

Phenotypes & Variants

Disruption phenotype

No glucokinase activity, no growth phenotype in an otherwise wild-type cell.

Chemistry

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00002151241-321Glucokinase

Proteomic databases

Expression

Induction

Constitutively expressed, weakly repressed by growth on glucose, repression may be partially controlled by cra (fruR).

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the bacterial glucokinase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    321
  • Mass (Da)
    34,723
  • Last updated
    2005-03-29 v1
  • Checksum
    2D6CD956641A3823
MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAVIRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFTAVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGHVDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDITERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASGFRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict108in Ref. 1; AAA64506
Sequence conflict188-189in Ref. 1; AAA64506
Sequence conflict274in Ref. 1; AAA64506

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U22490
EMBL· GenBank· DDBJ
AAA64506.1
EMBL· GenBank· DDBJ
Genomic DNA
U00096
EMBL· GenBank· DDBJ
AAC75447.1
EMBL· GenBank· DDBJ
Genomic DNA
AP009048
EMBL· GenBank· DDBJ
BAA16258.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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