P0A6V5 · GLPE_ECOLI
- ProteinThiosulfate sulfurtransferase GlpE
- GeneglpE
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids108 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Catalyzes, although with low efficiency, the sulfur transfer reaction from thiosulfate to cyanide. The relatively low affinity of GlpE for both thiosulfate and cyanide suggests that these compounds are not the physiological substrates. Thioredoxin 1 or related dithiol proteins could instead be the physiological sulfur-acceptor substrate. Possible association with the metabolism of glycerol-phosphate remains to be elucidated.
Miscellaneous
Incubation of GlpE with the cysteine-specific modifying reagent DTNB resulted in a greater-than-90% loss of activity.
Catalytic activity
- hydrogen cyanide + thiosulfate = 2 H+ + sulfite + thiocyanateThis reaction proceeds in the forward direction.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 65 | Cysteine persulfide intermediate | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | thiosulfate sulfurtransferase activity | |
Biological Process | glycerol metabolic process | |
Biological Process | sulfur utilization |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameThiosulfate sulfurtransferase GlpE
- EC number
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionP0A6V5
- Secondary accessions
Proteomes
Subcellular Location
Expression
Induction
By infection by filamentous bacteriophages. Expression is positively regulated by cyclic AMP-cAMP receptor protein (cAMP-CRP).
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 17-105 | Rhodanese | ||||
Sequence: QEKEAVLVDIRDPQSFAMGHAVQAFHLTNDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPAEVA |
Sequence similarities
Belongs to the GlpE family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length108
- Mass (Da)12,082
- Last updated2005-03-29 v1
- Checksum18D5B461C2A8EF19
Sequence caution
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 108 | in Ref. 2 | ||||
Sequence: A → RNALYCPLLCGISDSNDVDDYLFC |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
M96795 EMBL· GenBank· DDBJ | AAC28165.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
X07520 EMBL· GenBank· DDBJ | CAA30397.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
M54940 EMBL· GenBank· DDBJ | AAA23889.1 EMBL· GenBank· DDBJ | Genomic DNA | Different initiation | |
U18997 EMBL· GenBank· DDBJ | AAA58223.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U00096 EMBL· GenBank· DDBJ | AAC76450.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP009048 EMBL· GenBank· DDBJ | BAE77867.1 EMBL· GenBank· DDBJ | Genomic DNA |