P0A698 · UVRA_ECOLI
- ProteinUvrABC system protein A
- GeneuvrA
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids940 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. When the presence of a lesion has been verified by UvrB, the UvrA molecules dissociate.
Miscellaneous
Binds about 2 zinc atoms/molecule.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | DNA repair complex | |
Cellular Component | excinuclease repair complex | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | DNA binding | |
Molecular Function | excinuclease ABC activity | |
Molecular Function | identical protein binding | |
Molecular Function | zinc ion binding | |
Biological Process | DNA repair | |
Biological Process | nucleotide-excision repair | |
Biological Process | nucleotide-excision repair, DNA damage recognition | |
Biological Process | nucleotide-excision repair, preincision complex assembly | |
Biological Process | response to radiation | |
Biological Process | SOS response |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameUvrABC system protein A
- Short namesUvrA protein
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionP0A698
- Secondary accessions
Proteomes
Subcellular Location
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 253 | Reduced activity. | ||||
Sequence: C → A, H, or S |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000093049 | 1-940 | UvrABC system protein A | |||
Sequence: MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDTLYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTITEIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKGEHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLAESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGACPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEAPWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSAVREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAGQRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGVMYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGVHGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLKDVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHFDKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRCEACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEAREFFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYILDEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGEILVSGTPETVAECEASHTARFLKPML |
Proteomic databases
Interaction
Subunit
Forms a heterotetramer with UvrB during the search for lesions (By similarity).
Interacts with TRCF (Mfd). UvrB and TRCF binding to UvrA could be mutually exclusive
Interacts with TRCF (Mfd). UvrB and TRCF binding to UvrA could be mutually exclusive
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P0A698 | atl P0AFP2 | 2 | EBI-552091, EBI-560039 | |
BINARY | P0A698 | mfd P30958 | 2 | EBI-552091, EBI-554211 | |
BINARY | P0A698 | uvrA P0A698 | 2 | EBI-552091, EBI-552091 | |
BINARY | P0A698 | uvrB P0A8F8 | 7 | EBI-552091, EBI-552176 |
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for zinc finger, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Zinc finger | 253-280 | C4-type | ||||
Sequence: CPICGYSMRELEPRLFSFNNPAGACPTC | ||||||
Domain | 310-587 | ABC transporter 1 | ||||
Sequence: WDRRNFYYFQMLKSLADHYKFDVEAPWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSAVREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAGQRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGVMYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGVHGGEVVAEGPLEAIMAVPESL | ||||||
Domain | 607-937 | ABC transporter 2 | ||||
Sequence: ANPEKVLKLTGARGNNLKDVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHFDKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRCEACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEAREFFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYILDEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGEILVSGTPETVAECEASHTARFLK | ||||||
Zinc finger | 740-766 | C4-type | ||||
Sequence: CEACQGDGVIKVEMHFLPDIYVPCDQC |
Sequence similarities
Belongs to the ABC transporter superfamily. UvrA family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length940
- Mass (Da)103,868
- Last updated1988-04-01 v1
- ChecksumD61AAEB6514B860C
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
M13495 EMBL· GenBank· DDBJ | AAA24754.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U00006 EMBL· GenBank· DDBJ | AAC43152.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U00096 EMBL· GenBank· DDBJ | AAC77028.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP009048 EMBL· GenBank· DDBJ | BAE78060.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
X01621 EMBL· GenBank· DDBJ | CAA25764.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
J01721 EMBL· GenBank· DDBJ | AAA24753.1 EMBL· GenBank· DDBJ | Genomic DNA |