P0A4E0 · RNH2_BRUSU
- ProteinRibonuclease HII
- GenernhB
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids220 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.
Catalytic activity
Cofactor
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )
Note: Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding.
Features
Showing features for binding site.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | ribonuclease H2 complex | |
Molecular Function | manganese ion binding | |
Molecular Function | RNA binding | |
Molecular Function | RNA-DNA hybrid ribonuclease activity | |
Biological Process | DNA replication, removal of RNA primer | |
Biological Process | mismatch repair |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameRibonuclease HII
- EC number
- Short namesRNase HII
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Hyphomicrobiales > Brucellaceae > Brucella/Ochrobactrum group > Brucella
Accessions
- Primary accessionP0A4E0
- Secondary accessions
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000111552 | 1-220 | Ribonuclease HII | |||
Sequence: MKRSASDSPLLFDLPLAPDFSQEQQLMKRGLKHIAGIDEAGRGPLAGPVVAAAVVLDQNDLPEGLDDSKRLTAARREALYEIILTKAITVSVASLSARSIDASDIRKAALEAMRRAVIGLTLKPCHALVDGRDVPPGLPCPGSALVKGDQRSVSIAAASIVAKVTRDRMMIRAGAAHPPYGLEIHAGYATQKHRAAIESEGPVPGLHRYTFAPIKGRFDC |
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 32-220 | RNase H type-2 | ||||
Sequence: KHIAGIDEAGRGPLAGPVVAAAVVLDQNDLPEGLDDSKRLTAARREALYEIILTKAITVSVASLSARSIDASDIRKAALEAMRRAVIGLTLKPCHALVDGRDVPPGLPCPGSALVKGDQRSVSIAAASIVAKVTRDRMMIRAGAAHPPYGLEIHAGYATQKHRAAIESEGPVPGLHRYTFAPIKGRFDC |
Sequence similarities
Belongs to the RNase HII family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length220
- Mass (Da)23,385
- Last updated2005-03-15 v1
- Checksum87E3AB0664844E8E
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE014291 EMBL· GenBank· DDBJ | AAN29332.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002997 EMBL· GenBank· DDBJ | AEM17745.1 EMBL· GenBank· DDBJ | Genomic DNA |