P0A334 · KCSA_STRLI
- ProteinpH-gated potassium channel KcsA
- GenekcsA
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids160 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Acts as a pH-gated potassium ion channel; changing the cytosolic pH from 7 to 4 opens the channel, although it is not clear if this is the physiological stimulus for channel opening. Monovalent cation preference is K+ > Rb+ > NH4+ >> Na+ > Li+.
Miscellaneous
The amino acids 62-79 are situated in the membrane and are important for channel structure and properties.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | plasma membrane | |
Molecular Function | identical protein binding | |
Molecular Function | voltage-gated potassium channel activity |
Keywords
- Molecular function
- Biological process
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended namepH-gated potassium channel KcsA
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Kitasatosporales > Streptomycetaceae > Streptomyces
Accessions
- Primary accessionP0A334
- Secondary accessions
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane, intramembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-27 | Cytoplasmic | ||||
Sequence: MPPMLSGLLARLVKLLLGRHGSALHWR | ||||||
Transmembrane | 28-50 | Helical | ||||
Sequence: AAGAATVLLVIVLLAGSYLAVLA | ||||||
Topological domain | 51-61 | Extracellular | ||||
Sequence: ERGAPGAQLIT | ||||||
Intramembrane | 62-72 | Helical; Pore-forming | ||||
Sequence: YPRALWWSVET | ||||||
Intramembrane | 73-80 | Pore-forming | ||||
Sequence: ATTVGYGD | ||||||
Topological domain | 81-87 | Extracellular | ||||
Sequence: LYPVTLW | ||||||
Transmembrane | 88-111 | Helical | ||||
Sequence: GRLVAVVVMVAGITSFGLVTAALA | ||||||
Topological domain | 112-160 | Cytoplasmic | ||||
Sequence: TWFVGREQERRGHFVRHSEKAAEEAYTRTTRALHERFDRLERMLDDNRR |
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Cells grow slower and to lower myceliar densities.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 71 | Prevents channel inactivation. | ||||
Sequence: E → A |
Chemistry
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000054102 | 1-160 | pH-gated potassium channel KcsA | |||
Sequence: MPPMLSGLLARLVKLLLGRHGSALHWRAAGAATVLLVIVLLAGSYLAVLAERGAPGAQLITYPRALWWSVETATTVGYGDLYPVTLWGRLVAVVVMVAGITSFGLVTAALATWFVGREQERRGHFVRHSEKAAEEAYTRTTRALHERFDRLERMLDDNRR |
Interaction
Subunit
Homotetramer.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P0A334 | kcsA P0A334 | 37 | EBI-7262059, EBI-7262059 |
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 75-80 | Selectivity filter | ||||
Sequence: TVGYGD |
Domain
The cytoplasmic C-terminus is involved in the gating mechanism.
Sequence similarities
Belongs to the potassium channel family.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length160
- Mass (Da)17,694
- Last updated2005-03-15 v1
- ChecksumDEBD9E64384BF40C
Keywords
- Technical term