P0A2I5 · PARE_SALTY

Function

function

Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA (PubMed:22916023).
Performs the decatenation events required during the replication of a circular DNA molecule

Catalytic activity

  • ATP-dependent breakage, passage and rejoining of double-stranded DNA.
    EC:5.6.2.2 (UniProtKB | ENZYME | Rhea)

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Ca2+ (UniProtKB | Rhea| CHEBI:29108 )

Note: Binds two Mg2+ per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn2+ or Ca2+.

Features

Showing features for binding site, site.

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TypeIDPosition(s)Description
Binding site5ATP (UniProtKB | ChEBI)
Binding site42ATP (UniProtKB | ChEBI)
Binding site69ATP (UniProtKB | ChEBI)
Binding site110-116ATP (UniProtKB | ChEBI)
Binding site334ATP (UniProtKB | ChEBI)
Binding site418Mg2+ 1 (UniProtKB | ChEBI); catalytic
Site443Interaction with DNA
Site446Interaction with DNA
Binding site490Mg2+ 1 (UniProtKB | ChEBI); catalytic
Binding site490Mg2+ 2 (UniProtKB | ChEBI)
Binding site492Mg2+ 2 (UniProtKB | ChEBI)
Site497Interaction with DNA
Site615Interaction with DNA

GO annotations

AspectTerm
Cellular Componentchromosome
Molecular FunctionATP binding
Molecular FunctionDNA binding
Molecular FunctionDNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
Molecular Functionmetal ion binding
Biological Processchromosome segregation
Biological ProcessDNA topological change

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA topoisomerase 4 subunit B
  • EC number
  • Alternative names
    • Topoisomerase IV subunit B

Gene names

    • Name
      parE
    • Ordered locus names
      STM3181

Organism names

Accessions

  • Primary accession
    P0A2I5
  • Secondary accessions
    • P31598

Proteomes

Subcellular Location

Phenotypes & Variants

Features

Showing features for natural variant, mutagenesis.

TypeIDPosition(s)Description
Natural variant67in temperature-sensitive mutants parE206 and parE374
Mutagenesis67In parE206TS; a temperature-sensitive mutant, 33% decreased growth rate, slightly decreased negative supercoiling at permissive temperature.
Natural variant399in temperature-sensitive mutant parE377
Natural variant583in temperature-sensitive mutant parE493

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 3 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001454291-630DNA topoisomerase 4 subunit B

Proteomic databases

Interaction

Subunit

Heterotetramer composed of ParC and ParE.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain412-525Toprim

Sequence similarities

Belongs to the type II topoisomerase family. ParE type 1 subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    630
  • Mass (Da)
    70,089
  • Last updated
    2005-03-15 v1
  • Checksum
    7F2FCC25EB830019
MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSVDEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSNKNYQFSGGLHGVGISVVNALSKRVEVTVRRDGQVYNIAFENGEKVQDLQVVGTCGKRNTGTSVHFWPDESFFDSPRFSVSRLMHVLKAKAVLCPGVEITFKDEVNNSEQRWCYQDGLNDYLGEAVNGLPTLPEKPFIGNFNGETEAVDWALLWLPEGGELLTESYVNLIPTMQGGTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTKERLSSRQCAAFVSGVVKDAFSLWLNQNVQAAEQLAEMAIASAQRRLRAAKKVVRKKLTSGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEVSSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVRHFRALVKNGHVYVALPPLYRIDLGKEVYYALTEEEKAGVLEQLKRKKGKPNVQRFKGLGEMNPMQLRETTLDPNTRRLVQLTISDEDDQRTNAMMDMLLAKKRSEDRRNWLQEKGDLADLDV

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict305in Ref. 1; AAA27182
Sequence conflict333in Ref. 1; AAA27182
Sequence conflict383in Ref. 1; AAA27182

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
L05544
EMBL· GenBank· DDBJ
AAA27182.1
EMBL· GenBank· DDBJ
Genomic DNA
AE006468
EMBL· GenBank· DDBJ
AAL22055.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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