P0A182 · QADG_PSEPU

Function

function

Catalyzes the oxidative deamination of a wide range of aliphatic monoamines and diamines (PubMed:27315927).
The physiological electron acceptor is an azurin-like blue protein (PubMed:27315927).

Caution

The oxidation form of Met-30 is subject of controversy and could be the artifactual result of sample handling.

Catalytic activity

Cofactor

cysteine tryptophylquinone residue (UniProtKB | Rhea| CHEBI:20252 )

Note: Contains 1 cysteine tryptophylquinone per subunit.

Activity regulation

Inhibited by various carbonyl reagents, such as p-nitrophenylhydrazine (pNPH), 2,4-dinitrophenylhydrazine, semicarbazide and hydroxylamine.

pH Dependence

Optimum pH is around 8.0 for n-butylamine oxidation.

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site33Proton acceptor

GO annotations

AspectTerm
Cellular Componentperiplasmic space
Molecular Functionamine dehydrogenase activity

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Quinohemoprotein amine dehydrogenase subunit gamma
  • EC number
  • Short names
    QH-AmDH
  • Alternative names
    • Quinohemoprotein amine dehydrogenase 9 kDa subunit
    • Quinohemoprotein amine dehydrogenase catalytic subunit

Gene names

    • Name
      qhnDH

Organism names

  • Taxonomic identifier
  • Strains
    • ATCC 12633 / DSM 291 / JCM 13063 / CCUG 12690 / LMG 2257 / NBRC 14164 / NCIMB 9494 / NCTC 10936 / VKM B-2187 / Stanier 90
    • IFO 15633
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Pseudomonadales > Pseudomonadaceae > Pseudomonas

Accessions

  • Primary accession
    P0A182
  • Secondary accessions
    • Q88HA0
    • Q8VW83

Subcellular Location

Periplasm
Note: Is probably co-translocated into the periplasm when associated with the alpha and/or beta subunit, which contain both a signal peptide.

Keywords

Phenotypes & Variants

Features

Showing features for natural variant.

TypeIDPosition(s)Description
Natural variant62in strain: IFO 15366
Natural variant66in strain: IFO 15366

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 2 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for initiator methionine, chain, cross-link, modified residue.

TypeIDPosition(s)Description
Initiator methionine1Removed
ChainPRO_00002205512-79Quinohemoprotein amine dehydrogenase subunit gamma
Cross-link7↔164-cysteinyl-glutamic acid (Cys-Glu)
Cross-link27↔333-cysteinyl-aspartic acid (Cys-Asp)
Cross-link37↔434'-cysteinyl-tryptophylquinone (Cys-Trp)
Cross-link41↔493-cysteinyl-aspartic acid (Cys-Asp)
Modified residue43Tryptophylquinone

Post-translational modification

The cysteine tryptophylquinone (CTQ) is generated by oxidation of the indole ring of a tryptophan residue to form tryptophylquinone, followed by covalent cross-linking with a cysteine residue.

Keywords

Interaction

Subunit

Heterotrimer of an alpha, a beta and a gamma subunit.

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    79
  • Mass (Da)
    8,597
  • Last updated
    2007-01-23 v2
  • Checksum
    3B7EA9B9A4AB7298
MSAVAGCTATTDPGWEVDAFGGVSSLCQPMEADLYGCSDPCWWPAQVPDMMSTYQDWNAQASNSAEDWRNLGTVFPKDK

Mass Spectrometry

Molecular mass is 8,486.5 Da. Determined by Electrospray.
Molecular mass is 8,504 Da. Determined by Electrospray. With methionine sulfoxide.

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AB063331
EMBL· GenBank· DDBJ
BAB72010.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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