P0A176 · MNME_PSEPU

Function

function

Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34.

Cofactor

K+ (UniProtKB | Rhea| CHEBI:29103 )

Note: Binds 1 potassium ion per subunit.

Features

Showing features for binding site.

145650100150200250300350400450
TypeIDPosition(s)Description
Binding site24(6S)-5-formyl-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI)
Binding site81(6S)-5-formyl-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI)
Binding site120(6S)-5-formyl-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI)
Binding site226K+ (UniProtKB | ChEBI)
Binding site226-231GTP (UniProtKB | ChEBI)
Binding site230Mg2+ (UniProtKB | ChEBI)
Binding site245K+ (UniProtKB | ChEBI)
Binding site245-251GTP (UniProtKB | ChEBI)
Binding site247K+ (UniProtKB | ChEBI)
Binding site250K+ (UniProtKB | ChEBI)
Binding site251Mg2+ (UniProtKB | ChEBI)
Binding site270-273GTP (UniProtKB | ChEBI)
Binding site335-338GTP (UniProtKB | ChEBI)
Binding site456(6S)-5-formyl-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular FunctionGTP binding
Molecular FunctionGTPase activity
Molecular Functionmetal ion binding
Biological ProcesstRNA methylation
Biological ProcesstRNA wobble uridine modification

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA modification GTPase MnmE
  • EC number

Gene names

    • Name
      mnmE
    • Synonyms
      thdF
      , trmE

Organism names

Accessions

  • Primary accession
    P0A176
  • Secondary accessions
    • P25755

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001889061-456tRNA modification GTPase MnmE

Interaction

Subunit

Homodimer. Heterotetramer of two MnmE and two MnmG subunits.

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain216-379TrmE-type G

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    456
  • Mass (Da)
    48,963
  • Last updated
    2005-03-01 v1
  • Checksum
    B5E10A71C3FC5622
MNTVRETIAAIATAQGRGGVGIVRLSGPLAAKAGLLITGRTLTPRHAHYGPFRDDEGLVLDEGIALFFPGPNSFTGEDVLELQGHGGPVVLDMLLQRCVQVGCRLARPGEFSERAFLNDKLDLAQAEAIADLIEASSSQAARNALRSLQGEFSRRVHSLTEALIALRIYVEAAIDFPEEEIDFLADGHVLSMLDAVRSELSTVQREAGQGALLRDGMTVVIAGRPNAGKSSLLNQLAGREAAIVTDIAGTTRDILREHIHIDGMPLHVVDTAGLRDTDDHVEKIGVERALKAIGEADRVLLVVDSTAPEASDPFALWPEFLAQRPDPAKVTLIRNKADLSGERVALEQCDDGHVTITLSAKGDDTGLQLLRDHLKGCMGYEQTAESGFSARRRHLDALRQASEHLEHGRAQLTLAGAGELLAEDLRQAQHALGEITGAFSSDDLLGRIFSSFCIGK

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X62540
EMBL· GenBank· DDBJ
CAA44418.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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