P09883 · CEA9_ECOLX
- ProteinColicin-E9
- Genecol
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids582 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
This plasmid-coded bactericidal protein is an endonuclease active on both single- and double-stranded DNA but with undefined specificity.
Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria.
Features
Showing features for binding site.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | extrachromosomal circular DNA | |
Cellular Component | protein-containing complex | |
Molecular Function | endonuclease activity | |
Molecular Function | metal ion binding | |
Molecular Function | protein domain specific binding | |
Biological Process | defense response to bacterium | |
Biological Process | killing of cells of another organism |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameColicin-E9
- EC number
Gene names
Encoded on
- Plasmid ColE9
Organism names
- Organism
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionP09883
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000218684 | 1-582 | Colicin-E9 | |||
Sequence: MSGGDGRGHNTGAHSTSGNINGGPTGIGVSGGASDGSGWSSENNPWGGGSGSGIHWGGGSGRGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISASELSAAIAGIIAKLKKVNLKFTPFGVVLSSLIPSEIAKDDPNMMSKIVTSLPADDITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPGVFTASIPGAPVLNISVNDSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPKDSGHNAVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQANEDVARNQERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFNRFAHDPMAGGHRMWQMAGLKAQRAQTDVNNKQAAFDAAAKEKSDADAALSAAQERRKQKENKEKDAKDKLDKESKRNKPGKATGKGKPVGDKWLDDAGKDSGAPIPDRIADKLRDKEFKSFDDFRKAVWEEVSKDPELSKNLNPSNKSSVSKGYSPFTPKNQQVGGRKVYELHHDKPISQGGEVYDMDNIRVTTPKRHIDIHRGK |
Interaction
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P09883 | imm P04482 | 4 | EBI-1029888, EBI-15855054 | |
BINARY | P09883 | imm P13479 | 4 | EBI-1029888, EBI-1029882 | |
XENO | P09883 | ompF P02931 | 4 | EBI-1029888, EBI-371336 | |
XENO | P09883 | tolB P0A855 | 9 | EBI-1029888, EBI-7180728 |
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-74 | Disordered | ||||
Sequence: MSGGDGRGHNTGAHSTSGNINGGPTGIGVSGGASDGSGWSSENNPWGGGSGSGIHWGGGSGRGNGGGNGNSGGG | ||||||
Compositional bias | 9-24 | Polar residues | ||||
Sequence: HNTGAHSTSGNINGGP | ||||||
Compositional bias | 34-48 | Polar residues | ||||
Sequence: SDGSGWSSENNPWGG | ||||||
Region | 246-270 | Disordered | ||||
Sequence: SPGVTNNTDKDVRPAGFTQGGNTRD | ||||||
Region | 294-321 | Disordered | ||||
Sequence: PDQVKQRQDEENRRQQEWDATHPVEAAE | ||||||
Compositional bias | 296-321 | Basic and acidic residues | ||||
Sequence: QVKQRQDEENRRQQEWDATHPVEAAE | ||||||
Compositional bias | 422-457 | Basic and acidic residues | ||||
Sequence: ADAALSAAQERRKQKENKEKDAKDKLDKESKRNKPG | ||||||
Region | 422-489 | Disordered | ||||
Sequence: ADAALSAAQERRKQKENKEKDAKDKLDKESKRNKPGKATGKGKPVGDKWLDDAGKDSGAPIPDRIADK | ||||||
Compositional bias | 465-489 | Basic and acidic residues | ||||
Sequence: PVGDKWLDDAGKDSGAPIPDRIADK | ||||||
Region | 510-542 | Disordered | ||||
Sequence: SKDPELSKNLNPSNKSSVSKGYSPFTPKNQQVG | ||||||
Compositional bias | 515-539 | Polar residues | ||||
Sequence: LSKNLNPSNKSSVSKGYSPFTPKNQ |
Sequence similarities
Belongs to the colicin/pyosin nuclease family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length582
- Mass (Da)61,587
- Last updated1996-10-01 v4
- Checksum47A71B57B45AFDD9
Features
Showing features for compositional bias, sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 9-24 | Polar residues | ||||
Sequence: HNTGAHSTSGNINGGP | ||||||
Compositional bias | 34-48 | Polar residues | ||||
Sequence: SDGSGWSSENNPWGG | ||||||
Compositional bias | 296-321 | Basic and acidic residues | ||||
Sequence: QVKQRQDEENRRQQEWDATHPVEAAE | ||||||
Compositional bias | 422-457 | Basic and acidic residues | ||||
Sequence: ADAALSAAQERRKQKENKEKDAKDKLDKESKRNKPG | ||||||
Compositional bias | 465-489 | Basic and acidic residues | ||||
Sequence: PVGDKWLDDAGKDSGAPIPDRIADK | ||||||
Sequence conflict | 511 | in Ref. 3 | ||||
Sequence: K → R | ||||||
Compositional bias | 515-539 | Polar residues | ||||
Sequence: LSKNLNPSNKSSVSKGYSPFTPKNQ |
Keywords
- Technical term