P09786 · PHNB_PSEAE

Function

function

Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, a precursor for Pseudomonas quinolone signal (2-heptyl-3-hydroxy-4-quinolone; PQS) production which is required to induce the genes for the biosynthesis of the virulence factor pyocyanine (PCN), a characteristic blue-green phenazine pigment produced by P.aeruginosa (PubMed:11591691, PubMed:2153661, PubMed:23449919).
In the first step, the glutamine-binding beta subunit (PhnB) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (PhnA) to produce anthranilate (By similarity).

Miscellaneous

PhnAB is not feedback inhibited by tryptophan.

Catalytic activity

Pathway

Secondary metabolite biosynthesis; pyocyanine biosynthesis.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site56-58L-glutamine (UniProtKB | ChEBI)
Active site83Nucleophile; for GATase activity
Binding site87L-glutamine (UniProtKB | ChEBI)
Binding site133-134L-glutamine (UniProtKB | ChEBI)
Active site169For GATase activity
Active site171For GATase activity

GO annotations

AspectTerm
Molecular Functionanthranilate phosphoribosyltransferase activity
Molecular Functionanthranilate synthase activity
Biological Processglutamine metabolic process
Biological Processphenazine biosynthetic process
Biological Processtryptophan biosynthetic process

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Anthranilate synthase component 2, pyocyanine specific
  • EC number
  • Short names
    AS; ASII
  • Alternative names
    • Anthranilate synthase beta subunit, pyocyanine specific
    • Anthranilate synthase, GATase component
    • Anthranilate synthase, glutamine amidotransferase component

Gene names

    • Name
      phnB
    • Synonyms
      trpG
    • Ordered locus names
      PA1002

Organism names

Accessions

  • Primary accession
    P09786

Proteomes

Organism-specific databases

Phenotypes & Variants

Disruption phenotype

Single disruption in strain PA04290 results in decreased pyocyanine synthesis (PubMed:2153661).
Double phnA-phnB deletions are tryptophan prototrophs; they make less PQS (PubMed:23449919).

Features

Showing features for natural variant.

TypeIDPosition(s)Description
Natural variant148-149in strain: PAC174
Natural variant195in strain: PAC174

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 2 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000569061-200Anthranilate synthase component 2, pyocyanine specific

Proteomic databases

Interaction

Subunit

Heterotetramer consisting of two non-identical subunits: a beta subunit (PhnB) and a large alpha subunit (PhnA).

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain2-195Glutamine amidotransferase type-1

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    200
  • Mass (Da)
    21,845
  • Last updated
    2000-12-08 v3
  • Checksum
    9D9E218DDE8D7E30
MRITLLDNFDSFTYNLVEQFCLLGAEVRVMRNDTPLPTIQAALLADGCELLVLSPGPGRPEDAGCMLELLAWARGRLPVLGVCLGHQALALAAGGAVGEARKPLHGKSTSLRFDQRHPLFDGIADLRVARYHSLVVSRLPEGFDCLADADGEIMAMADPRNRQLGLQFHPESILTTHGQRLLENALLWCGALAVRERLRA

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict195in Ref. 1; AAA88449

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
M15733
EMBL· GenBank· DDBJ
AAA26018.1
EMBL· GenBank· DDBJ
Genomic DNA
M33810
EMBL· GenBank· DDBJ
AAA88449.1
EMBL· GenBank· DDBJ
Genomic DNA
M33811
EMBL· GenBank· DDBJ
AAA88452.1
EMBL· GenBank· DDBJ
Genomic DNA
AE004091
EMBL· GenBank· DDBJ
AAG04391.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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