P08556 · RASN_MOUSE

  • Protein
    GTPase NRas
  • Gene
    Nras
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.

Catalytic activity

Activity regulation

Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).

Features

Showing features for binding site.

118920406080100120140160180
TypeIDPosition(s)Description
Binding site10-18GTP (UniProtKB | ChEBI)
Binding site29-30GTP (UniProtKB | ChEBI)
Binding site57-61GTP (UniProtKB | ChEBI)
Binding site116-119GTP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular ComponentGolgi membrane
Cellular Componentmembrane
Cellular Componentplasma membrane
Molecular FunctionG protein activity
Molecular FunctionGDP binding
Molecular FunctionGTP binding
Molecular FunctionGTPase activity
Molecular Functionprotein-containing complex binding
Biological Processdefense response to protozoan
Biological Processnegative regulation of skeletal muscle tissue development
Biological Processpositive regulation of angiogenesis
Biological Processpositive regulation of fibroblast proliferation
Biological Processpositive regulation of type II interferon production
Biological ProcessRas protein signal transduction
Biological Processregulation of cell cycle
Biological Processregulation of gene expression
Biological Processregulation of protein phosphorylation
Biological ProcessT cell receptor signaling pathway

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    GTPase NRas
  • EC number
  • Alternative names
    • Transforming protein N-Ras

Gene names

    • Name
      Nras

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    P08556

Proteomes

Organism-specific databases

Subcellular Location

Cell membrane
; Lipid-anchor
Golgi apparatus membrane
; Lipid-anchor
Note: Shuttles between the plasma membrane and the Golgi apparatus.

Keywords

Phenotypes & Variants

Keywords

PTM/Processing

Features

Showing features for chain, modified residue, cross-link, lipidation, propeptide.

TypeIDPosition(s)Description
ChainPRO_00000430101-186GTPase NRas
Modified residue89Phosphoserine
Cross-link170Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
Lipidation181S-palmitoyl cysteine
Lipidation186S-farnesyl cysteine
PropeptidePRO_0000043011187-189Removed in mature form

Post-translational modification

Palmitoylated by the ZDHHC9-GOLGA7 complex. Depalmitoylated by ABHD17A, ABHD17B and ABHD17C. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi.
Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs).
Ubiquitinated by the BCR(LZTR1) E3 ubiquitin ligase complex at Lys-170 in a non-degradative manner, leading to inhibit Ras signaling by decreasing Ras association with membranes.
Phosphorylation at Ser-89 by STK19 enhances NRAS-association with its downstream effectors.

Keywords

Proteomic databases

PTM databases

Interaction

Subunit

Interacts (active GTP-bound form preferentially) with RGS14. Interacts (active GTP-bound form) with RASSF7 (By similarity).

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Features

Showing features for motif, region.

TypeIDPosition(s)Description
Motif32-40Effector region
Region166-185Hypervariable region

Sequence similarities

Belongs to the small GTPase superfamily. Ras family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    189
  • Mass (Da)
    21,199
  • Last updated
    1988-08-01 v1
  • Checksum
    688D33F6814855B7
MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNSKSFADINLYREQIKRVKDSDDVPMVLVGNKCDLPTRTVDTKQAHELAKSYGIPFIETSAKTRQGVEDAFYTLVREIRQYRLKKLNSSDDGTQGCMGSPCVLM

Computationally mapped potential isoform sequences

There are 5 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
E9PVD5E9PVD5_MOUSENras46
Q9D091Q9D091_MOUSENras189
A0A0G2JGM2A0A0G2JGM2_MOUSENras74
A0A0G2JGP4A0A0G2JGP4_MOUSENras183
A0A0G2JE25A0A0G2JE25_MOUSENras150

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict189in Ref. 2; CAA31958

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
M12124
EMBL· GenBank· DDBJ
AAA39839.1
EMBL· GenBank· DDBJ
Genomic DNA
M12121
EMBL· GenBank· DDBJ
AAA39839.1
EMBL· GenBank· DDBJ
Genomic DNA
M12122
EMBL· GenBank· DDBJ
AAA39839.1
EMBL· GenBank· DDBJ
Genomic DNA
M12123
EMBL· GenBank· DDBJ
AAA39839.1
EMBL· GenBank· DDBJ
Genomic DNA
X13664
EMBL· GenBank· DDBJ
CAA31958.1
EMBL· GenBank· DDBJ
mRNA
X06909
EMBL· GenBank· DDBJ
CAA30010.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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