P08556 · RASN_MOUSE
- ProteinGTPase NRas
- GeneNras
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids189 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
Catalytic activity
- GTP + H2O = GDP + H+ + phosphate
Activity regulation
Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | Golgi membrane | |
Cellular Component | membrane | |
Cellular Component | plasma membrane | |
Molecular Function | G protein activity | |
Molecular Function | GDP binding | |
Molecular Function | GTP binding | |
Molecular Function | GTPase activity | |
Molecular Function | protein-containing complex binding | |
Biological Process | defense response to protozoan | |
Biological Process | negative regulation of skeletal muscle tissue development | |
Biological Process | positive regulation of angiogenesis | |
Biological Process | positive regulation of fibroblast proliferation | |
Biological Process | positive regulation of type II interferon production | |
Biological Process | Ras protein signal transduction | |
Biological Process | regulation of cell cycle | |
Biological Process | regulation of gene expression | |
Biological Process | regulation of protein phosphorylation | |
Biological Process | T cell receptor signaling pathway |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameGTPase NRas
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionP08556
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Lipid-anchor
Golgi apparatus membrane ; Lipid-anchor
Note: Shuttles between the plasma membrane and the Golgi apparatus.
Keywords
- Cellular component
Phenotypes & Variants
Keywords
- Disease
PTM/Processing
Features
Showing features for chain, modified residue, cross-link, lipidation, propeptide.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000043010 | 1-186 | GTPase NRas | |||
Sequence: MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNSKSFADINLYREQIKRVKDSDDVPMVLVGNKCDLPTRTVDTKQAHELAKSYGIPFIETSAKTRQGVEDAFYTLVREIRQYRLKKLNSSDDGTQGCMGSPC | ||||||
Modified residue | 89 | Phosphoserine | ||||
Sequence: S | ||||||
Cross-link | 170 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) | ||||
Sequence: K | ||||||
Lipidation | 181 | S-palmitoyl cysteine | ||||
Sequence: C | ||||||
Lipidation | 186 | S-farnesyl cysteine | ||||
Sequence: C | ||||||
Propeptide | PRO_0000043011 | 187-189 | Removed in mature form | |||
Sequence: VLM |
Post-translational modification
Palmitoylated by the ZDHHC9-GOLGA7 complex. Depalmitoylated by ABHD17A, ABHD17B and ABHD17C. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi.
Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs).
Ubiquitinated by the BCR(LZTR1) E3 ubiquitin ligase complex at Lys-170 in a non-degradative manner, leading to inhibit Ras signaling by decreasing Ras association with membranes.
Phosphorylation at Ser-89 by STK19 enhances NRAS-association with its downstream effectors.
Keywords
- PTM
Proteomic databases
PTM databases
Structure
Family & Domains
Features
Showing features for motif, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 32-40 | Effector region | ||||
Sequence: YDPTIEDSY | ||||||
Region | 166-185 | Hypervariable region | ||||
Sequence: YRLKKLNSSDDGTQGCMGSP |
Sequence similarities
Belongs to the small GTPase superfamily. Ras family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length189
- Mass (Da)21,199
- Last updated1988-08-01 v1
- Checksum688D33F6814855B7
Computationally mapped potential isoform sequences
There are 5 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
E9PVD5 | E9PVD5_MOUSE | Nras | 46 | ||
Q9D091 | Q9D091_MOUSE | Nras | 189 | ||
A0A0G2JGM2 | A0A0G2JGM2_MOUSE | Nras | 74 | ||
A0A0G2JGP4 | A0A0G2JGP4_MOUSE | Nras | 183 | ||
A0A0G2JE25 | A0A0G2JE25_MOUSE | Nras | 150 |
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 189 | in Ref. 2; CAA31958 | ||||
Sequence: M → MCKTL |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
M12124 EMBL· GenBank· DDBJ | AAA39839.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
M12121 EMBL· GenBank· DDBJ | AAA39839.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
M12122 EMBL· GenBank· DDBJ | AAA39839.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
M12123 EMBL· GenBank· DDBJ | AAA39839.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
X13664 EMBL· GenBank· DDBJ | CAA31958.1 EMBL· GenBank· DDBJ | mRNA | ||
X06909 EMBL· GenBank· DDBJ | CAA30010.1 EMBL· GenBank· DDBJ | Genomic DNA |