P08395 · SPPA_ECOLI
- ProteinProtease 4
- GenesppA
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids618 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Digests cleaved signal peptides in vitro, its in vivo function is unknown. This activity is necessary to maintain proper secretion of mature proteins across the membrane.
Activity regulation
Inhibited by serine hydrolase inhibitor FP-biotin and by antipain.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 209 | Proton donor/acceptor | ||||
Sequence: K | ||||||
Active site | 409 | Nucleophile | ||||
Sequence: S |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | membrane | |
Cellular Component | plasma membrane | |
Molecular Function | endopeptidase activity | |
Molecular Function | serine-type peptidase activity | |
Biological Process | signal peptide processing |
Keywords
- Molecular function
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameProtease 4
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionP08395
- Secondary accessions
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Cell inner membrane ; Single-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-24 | Cytoplasmic | ||||
Sequence: MRTLWRFIAGFFKWTWRLLNFVRE | ||||||
Transmembrane | 25-45 | Helical | ||||
Sequence: MVLNLFFIFLVLVGVGIWMQV | ||||||
Topological domain | 46-618 | Periplasmic | ||||
Sequence: SGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVNTIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQYYLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRDDMSPAAREADSRWIGELWQNYLNTVAANRQIPAEQVFPGAQGLLEGLTKTGGDTAKYALENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFANGAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAARAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGVHTDGVSTSPLADVSITRALPPEAQLMMQLSIENGYKRFITLVADARHSTPEQIDKIAQGHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSGSVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANMR |
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
No effect on processing of liberated signal peptides in vivo.
Features
Showing features for natural variant, mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Natural variant | 151 | in strain: ECOR 49 and ECOR 50 | ||||
Sequence: V → I | ||||||
Natural variant | 186 | in strain: ECOR 16 | ||||
Sequence: G → S | ||||||
Mutagenesis | 209 | Loss of activity. | ||||
Sequence: K → A | ||||||
Natural variant | 252 | in strain: ECOR 16 | ||||
Sequence: E → H | ||||||
Natural variant | 252 | in strain: ECOR 49 | ||||
Sequence: E → K | ||||||
Natural variant | 252 | in strain: ECOR 38, ECOR 39, ECOR 40, ECOR 50, ECOR 65 and ECOR 68 | ||||
Sequence: E → Q | ||||||
Natural variant | 294 | in strain: ECOR 38, ECOR 39, ECOR 40, ECOR 49, ECOR 50 and ECOR 65 | ||||
Sequence: A → T | ||||||
Mutagenesis | 366 | Reduced activity. | ||||
Sequence: K → A | ||||||
Mutagenesis | 409 | Loss of activity. | ||||
Sequence: S → A | ||||||
Mutagenesis | 410 | Reduced activity. | ||||
Sequence: G → A | ||||||
Mutagenesis | 412 | No effect on activity. | ||||
Sequence: Y → F | ||||||
Mutagenesis | 510 | Reduced activity. | ||||
Sequence: H → A | ||||||
Mutagenesis | 524 | Increased activity. | ||||
Sequence: D → N |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 6 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000171438 | 1-618 | Protease 4 | |||
Sequence: MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQVSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVNTIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQYYLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRDDMSPAAREADSRWIGELWQNYLNTVAANRQIPAEQVFPGAQGLLEGLTKTGGDTAKYALENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFANGAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAARAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGVHTDGVSTSPLADVSITRALPPEAQLMMQLSIENGYKRFITLVADARHSTPEQIDKIAQGHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSGSVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANMR |
Proteomic databases
Structure
Family & Domains
Domain
A tandem duplication in the protein fold creates an intact active site between the repeated domains of each monomer.
Sequence similarities
Belongs to the peptidase S49 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length618
- Mass (Da)67,219
- Last updated2003-08-29 v2
- ChecksumEAF00496B5946AC3
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 378 | in Ref. 1; AAA24648 and 5; AAA57009 | ||||
Sequence: S → T |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
M13359 EMBL· GenBank· DDBJ | AAA24648.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U00096 EMBL· GenBank· DDBJ | AAC74836.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP009048 EMBL· GenBank· DDBJ | BAA15557.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U13772 EMBL· GenBank· DDBJ | AAA57008.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U13773 EMBL· GenBank· DDBJ | AAA57009.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U13774 EMBL· GenBank· DDBJ | AAA57010.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U13775 EMBL· GenBank· DDBJ | AAA57011.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U13776 EMBL· GenBank· DDBJ | AAA57012.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U13777 EMBL· GenBank· DDBJ | AAA57013.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U13778 EMBL· GenBank· DDBJ | AAA57014.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U13779 EMBL· GenBank· DDBJ | AAA57015.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U13780 EMBL· GenBank· DDBJ | AAA57016.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U13782 EMBL· GenBank· DDBJ | AAA57017.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U13833 EMBL· GenBank· DDBJ | AAA57030.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U13834 EMBL· GenBank· DDBJ | AAA57031.1 EMBL· GenBank· DDBJ | Genomic DNA |